Journal
CARDIOVASCULAR RESEARCH
Volume 82, Issue 1, Pages 51-58Publisher
OXFORD UNIV PRESS
DOI: 10.1093/cvr/cvp039
Keywords
K-ATP channel; Caveolin-3; Adenosine receptor
Categories
Funding
- American Heart Association National Center [0835117N]
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ATP-sensitive potassium (K-ATP) channels in the heart are critical regulators of cellular excitability and action potentials during ischaemia. However, little is known about subcellular localization of these channels and their regulation. The present study was designed to explore the potential role of caveolae in the regulation of K-ATP channels in cardiac ventricular myocytes. Both adult and neonatal rat cardiomyocytes were used. Subcellular fractionation by density gradient centrifugation, western blotting, co-immunoprecipitation, and immunofluorescence confocal microscopy were employed in combination with whole-cell voltage clamp recordings and siRNA gene silencing. We detected that the majority of K-ATP channels on the plasma membrane of cardiac myocytes were localized in caveolin-3-enriched microdomains by cell fractionation and ultracentrifugation followed by western blotting. Immunofluorescence confocal microscopy revealed extensive colocalization of K-ATP channel pore-forming subunit Kir6.2 and caveolin-3 along the plasma membrane. Co-immunoprecipitation of cardiac myocytes showed significant association of Kir6.2, adenosine A(1) receptors, and caveolin-3. Furthermore, whole-cell voltage clamp studies suggested that adenosine A(1) receptor-mediated activation of K-ATP channels was largely eliminated by disrupting caveolae with methyl-beta-cyclodextrin or by small interfering RNA, whereas pinacidil-induced K-ATP activation was not altered. We demonstrate that K-ATP channels are localized to caveolin-enriched microdomains. This microdomain association is essential for adenosine receptor-mediated regulation of K-ATP channels in cardiac myocytes.
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