Article
Biochemistry & Molecular Biology
Ida Joely Jacobs, Qiaoli Li
Summary: Pseudoxanthoma elasticum (PXE) is a heritable calcification disorder. This study found that combination therapy with etidronate and minocycline could further reduce ectopic calcification in a PXE mouse model and decrease serum alkaline phosphatase activity. These results suggest that combination therapy might be an effective treatment approach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pathology
Douglas Ralph, Koen van de Wetering, Jouni Uitto, Qiaoli Li
Summary: Pathologic soft tissue calcification is a serious condition with genetic and acquired causes. This review discusses the underlying genetic defects in monogenic disorders of pathologic calcification and suggests that PPi-targeted therapies may be beneficial in treating these conditions.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Nutrition & Dietetics
Pilar Sanchis, Angel-Arturo Lopez-Gonzalez, Antonia Costa-Bauza, Carla Busquets-Cortes, Pere Riutord, Paula Calvo, Felix Grases
Summary: Inositol hexaphosphate (phytate; IP6) is a natural compound that can inhibit crystal development and dissolution, potentially offering protective effects against bone-related diseases like osteoporosis. Studies have shown that phytate consumption may help increase bone mineral density and prevent bone-loss disorders.
Article
Cardiac & Cardiovascular Systems
Bastien Poitier, Jeanne Rancic, Ulysse Richez, Julie Piquet, Salma El Batti, David M. M. Smadja
Summary: This study aimed to investigate whether fibrin deposition could lead to calcification in bovine pericardium tissue. The results showed that fibrin-treated tissue had higher levels of calcification and more inflammatory-like cell infiltration. The conclusion is that fibrin deposition may be one of the triggers for calcification and deterioration of heart valves.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Medicine, Research & Experimental
Elena L. Dent, Michael J. Ryan
Summary: This article reviews a seminal Clinical Science paper published in 1972, which made significant contributions to the understanding of whole-body calcium regulation and paved the way for the development of drugs for the treatment of osteoporosis, cancer metastasis, and other calcium disorders.
Article
Biochemical Research Methods
Stefan Lundkvist, Fatemeh Niaziorimi, Flora Szeri, Matthew Caffet, Sharon F. Terry, Gunnar Johansson, Robert S. Jansen, Koen van de Wetering
Summary: Inorganic pyrophosphate (PPi) is a crucial regulator of extracellular mineralization, but there is currently no reliable assay for its quantification. In this study, we report a new and robust assay that converts PPi into ATP and quantifies it using firefly luciferase-based bioluminescence. The assay shows high sensitivity, precision, and accuracy, and outperforms currently available assays.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Audrey Laurain, Isabelle Rubera, Micheline Razzouk-Cadet, Stephanie Bonnafous, Miguel Albuquerque, Valerie Paradis, Stephanie Patouraux, Christophe Duranton, Olivier Lesaux, Georges Leftheriotis, Albert Tran, Rodolphe Anty, Philippe Gual, Antonio Iannelli, Guillaume Favre
Summary: Liver fibrosis is associated with arterial calcification. Plasma inorganic pyrophosphate (PPi) levels are correlated with liver fibrosis, liver function, and arterial calcification. Liver transplantation can restore serum albumin levels and PPi levels.
Review
Cardiac & Cardiovascular Systems
David F. Williams, Deon Bezuidenhout, Jandre de Villiers, Paul Human, Peter Zilla
Summary: The use of bioprosthetic valves in heart valve therapy has evolved over the decades, with a focus on improving production methods to address issues like immune responses and calcification. Recent advancements in cross-linking and decellularization techniques have improved durability, but the need for even longer-lasting and safer treatments for younger patients has become a crucial clinical and social issue. Developing alternative treatment protocols focusing on tissue-protective decellularization and finely-titrated cross-linking sequences show promise for enhancing the longevity of pericardial heart valves.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Hussein M. El-Husseiny, Eman A. Mady, Masahiro Kaneda, Kazumi Shimada, Yasumoto Nakazawa, Tatsuya Usui, Mohamed Elbadawy, Yusuke Ishihara, Moeko Hirose, Yohei Kamei, Ahmed S. Doghish, Hesham A. El-Mahdy, Walaa A. El-Dakroury, Ryou Tanaka
Summary: The use of Trypsin + TX + SDS protocol successfully decellularized bovine pericardium, porcine pericardium, and porcine tunica vaginalis, preserving extracellular matrix integrity and demonstrating good biocompatibility. The decellularized porcine pericardium showed increased elasticity, while the tensile strength of decellularized bovine pericardium and porcine tunica vaginalis remained unaffected. Decellularized tissues supported the growth of adipose stem cells and showed in vivo biocompatibility and degradability. Trypsin + TX + SDS is a successful decellularization protocol, and decellularized pericardia and tunica vaginalis are promising scaffolds for tissue engineering applications.
Review
Biochemistry & Molecular Biology
Ricardo Villa-Bellosta
Summary: Cardiovascular complications due to accelerated arterial stiffening and atherosclerosis are the leading cause of morbimortality in Western society, often associated with vascular calcification. The balance between phosphate and pyrophosphate, strictly controlled by several genes, plays a key role in calcification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Multidisciplinary
Martina Todesco, Saima Jalil Imran, Tiago Moderno Fortunato, Deborah Sandrin, Giulia Borile, Filippo Romanato, Martina Casarin, Germana Giuggioli, Fabio Conte, Massimo Marchesan, Gino Gerosa, Andrea Bagno
Summary: Human and animal pericardium are commonly used materials for repairing damaged tissues in cardiovascular surgery, but they have drawbacks such as calcification and limited availability. Decellularized pericardium, which removes cellular and nuclear components, shows potential as a scaffold for cardiovascular tissue regeneration. This study evaluates the effects of a new detergent on decellularizing bovine and porcine pericardium, and finds that the decellularized pericardium retains its extracellular matrix structure without cytotoxic effects.
Article
Pharmacology & Pharmacy
Kanan Bando, Takefumi Oizumi, Tetsu Takahashi, Itaru Mizoguchi, Shunji Sugawara, Yasuo Endo
Summary: Nitrogen-containing bisphosphonates (NBPs) exhibit stronger anti-bone-resorptive activities than non-nitrogen-containing bisphosphonates (non-NBPs), potentially causing osteonecrosis of the jaw (BRONJ). Preliminary clinical studies suggest that etidronate (Eti) may be useful for treating BRONJ. Research results indicate that Eti and clodronate (Clo) can reduce or prevent the inflammatory/necrotic effects of NBPs.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Biochemistry & Molecular Biology
Felix Grases, Antonia Costa-Bauza, Paula Calvo, Francesca Julia, Jaume Dietrich, Rosa Maria Gomila, Gabriel Martorell, Pilar Sanchis
Summary: Phytate consumption has no adverse effects on a balanced diet, and has been found to prevent pathological calcifications. Hydrolysis of phytate produces inositol phosphates (InsPs) that also have biological activity. InsP6 and its hydrolysis products effectively inhibit calcium oxalate crystallization. It is important to develop simple analytical methods to quantify the level of individual InsPs.
Review
Biochemistry & Molecular Biology
Briana K. Shimada, Viola Pomozi, Janna Zoll, Sheree Kuo, Ludovic Martin, Olivier Le Saux
Summary: ABCC6 plays a crucial role in the pathogenesis of PXE and other calcification disorders by regulating ATP efflux and inhibiting calcification. Current therapies only aim to alleviate symptoms, but novel approaches have been developed through extensive research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hongchun Liu, Jiazhen Tu
Summary: The study showed that using a toothpaste containing 10% high cleaning silica, 0.5% sodium phytate, and 0.5% sodium pyrophosphate for 8 weeks effectively reduced extrinsic tooth stain.
Article
Urology & Nephrology
Smeeta Sinha, Lisa J. Gould, Sagar U. Nigwekar, Thomas E. Serena, Vincent Brandenburg, Sharon M. Moe, George Aronoff, Dinesh K. Chatoth, Jeffrey L. Hymes, Stephan Miller, Claire Padgett, Kevin J. Carroll, Joan Perello, Alex Gold, Glenn M. Chertow
Summary: The CALCIPHYX trial is a Phase 3 clinical trial aimed at evaluating the efficacy and safety of SNF472 in patients with ulcerated calciphylaxis lesions who are receiving hemodialysis. The trial will assess endpoints such as wound healing, pain relief, and wound-related quality of life.
CLINICAL KIDNEY JOURNAL
(2022)
Article
Nutrition & Dietetics
Jose Ignacio Ramirez Manent, Barbara Altisench Jane, Pilar Sanchis Cortes, Carla Busquets-Cortes, Sebastiana Arroyo Bote, Luis Masmiquel Comas, Angel Arturo Lopez Gonzalez
Summary: This study assessed the impact of unhealthy lifestyles during lockdown on health parameters. The results showed that the unhealthy habits during lockdown resulted in negative effects on body weight, BMI, percentage of body fat, total cholesterol levels, LDL levels, and glucose levels, leading to a worsening of cardiovascular risk factors.
Article
Biochemistry & Molecular Biology
Felix Grases, Antonia Costa-Bauza, Paula Calvo, Francesca Julia, Jaume Dietrich, Rosa Maria Gomila, Gabriel Martorell, Pilar Sanchis
Summary: Phytate consumption has no adverse effects on a balanced diet, and has been found to prevent pathological calcifications. Hydrolysis of phytate produces inositol phosphates (InsPs) that also have biological activity. InsP6 and its hydrolysis products effectively inhibit calcium oxalate crystallization. It is important to develop simple analytical methods to quantify the level of individual InsPs.
Review
Biochemistry & Molecular Biology
Antelm Pujol, Pilar Sanchis, Felix Grases, Luis Masmiquel
Summary: Phytate, found in wholegrains, legumes, and oilseeds, is a recognized nutraceutical that has been shown to treat or prevent certain diseases. It has antioxidation and anti-inflammatory effects, inhibiting calcium salt crystallization, reducing vascular calcifications, and playing a role in bone mass loss treatment. Phytate also improves lipid and carbohydrate metabolism, reduces protein glycation, and shows promise in neurodegenerative disease treatment.
Article
Biochemistry & Molecular Biology
Aina M. Galmes-Panades, Miquel Bennasar-Veny, Paula Oliver, Natalia Garcia-Coll, Alice Chaplin, Sergio Fresneda, Laura Gallardo-Alfaro, Carmen Garcia-Ruano, Jadwiga Konieczna, Alfonso Leiva, Lluis Masmiquel, Catalina Pico, Ignacio Ricci-Cabello, Dora Romaguera, Rosmeri Rivera, Pilar Sanchis, Josep Vidal-Conti, Aina M. Yanez
Summary: This is a two-phase randomized controlled clinical trial aimed at assessing the effectiveness of different types and frequencies of physical exercise on glycaemic control in adults with prediabetes. The study will collect various biomedical data and questionnaires to evaluate the impact of interventions on health.
Article
Nutrition & Dietetics
Pilar Sanchis, Rafael Maria Prieto, Jadwiga Konieczna, Felix Grases, Itziar Abete, Jordi Salas-Salvado, Vicente Martin, Miguel Ruiz-Canela, Nancy Babio, Jesus Francisco Garcia-Gavilan, Albert Goday, Antonia Costa-Bauza, Jose Alfredo Martinez, Dora Romaguera
Summary: The main objective of this study was to examine the relationship between dietary phytate intake and bone mineral density (BMD) in a Mediterranean population of postmenopausal women. The results showed that phytate intake was positively associated with BMD in Mediterranean postmenopausal women, indicating a potential protective effect on bone. However, further large-scale, long-term, and randomized prospective clinical studies are needed to assess the potential benefits of phytate consumption on BMD in postmenopausal women.
Article
Nutrition & Dietetics
Iris Coello, Pilar Sanchis, Enrique C. Pieras, Felix Grases
Summary: Dietary choices can influence the formation of different types of kidney stones. Processed food and meat derivatives are associated with papillary COM stones, while consuming enough calcium may offer protection against non-papillary COM stones. Dairy product consumption is linked to COD stones. A diet high in animal items increases the risk of papillary COM stones, while consuming calcium or dairy products may be preventive or a risk factor for non-papillary COM and COD stones respectively.
Article
Endocrinology & Metabolism
Pilar Sanchis, Paula Calvo, Antelm Pujol, Rosmeri Rivera, Francisco Berga, Regina Fortuny, Antonia Costa-Bauza, Felix Grases, Luis Masmiquel
Summary: This study found that supplementation of phytate can increase plasma adiponectin concentration and decrease HbA1c levels in patients with type 2 diabetes mellitus (T2DM), which could have beneficial effects in preventing and minimizing diabetic-related complications.
NUTRITION & DIABETES
(2023)
Article
Urology & Nephrology
Francesca Julia, Antonia Costa-Bauza, Francisco Berga, Felix Grases
Summary: N-acetylcysteine showed a modest effect on the dissolution of uric acid stones, while theobromine significantly increased their dissolution. The combination of both substances had a similar effect to theobromine alone. Adding theobromine to a therapy that uses citrate and/or bicarbonate may be a new strategy for the oral chemolysis of uric acid stones.
WORLD JOURNAL OF UROLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Eric A. Shikatani, Tao Wang, Luke S. Dingwell, Colin White-Dzuro, Abdul Momen, Mansoor Husain
Summary: This study found that GDF5 deficiency can reduce the incidence of cardiac rupture after myocardial infarction and improve survival rates in mice. Additionally, GDF5 deficiency leads to increased myocardial fibrosis and decreased afterload. However, it also results in the occurrence of chronic adverse remodeling.
CARDIOVASCULAR PATHOLOGY
(2024)
Review
Cardiac & Cardiovascular Systems
Martin Kveton, Lukas Hudec, Ivan Vykopal, Matej Halinkovic, Miroslav Laco, Andrea Felsoova, Wanda Benesova, Ondrej Fabian
Summary: This comprehensive review explores the transformative role of digital pathology and computational pathology, especially through machine learning, in the field of heart transplantation. These methodologies have the potential to enhance diagnostic outcomes and reduce observer variability by extracting valuable information from large datasets beyond human perceptual capabilities. However, challenges such as limited sample sizes, diverse data sources, and the absence of standardized protocols hinder the widespread adoption of these techniques.
CARDIOVASCULAR PATHOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Abhinav Goyal, Andrew J. Layman, Melanie C. Bois, Joseph J. Maleszewski
Summary: This study examined the prevalence and characteristics of Lambl excrescences (LEs) in 403 healthy human hearts and found that the prevalence of LEs in healthy hearts is far lower than previously reported.
CARDIOVASCULAR PATHOLOGY
(2024)
