Article
Food Science & Technology
Lisa Holzl-Armstrong, Sarah Moody, Jill E. Kucab, Edwin P. Zwart, Medjda Bellamri, Mirjam Luijten, Robert J. Turesky, Michael R. Stratton, Volker M. Arlt, David H. Phillips
Summary: The metabolite of PhIP, N-OH-PhIP, induces G:C base pair transversions in the human TP53 gene, with a high frequency of G > T/C > A transversions. The mutational signature of N-OH-PhIP-treated clones shows similarity to signatures found in human tumors, indicating a potential link between PhIP exposure and cancer development.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Chemistry, Applied
Guoyu Sun, Pengpu Wang, Weina Chen, Xiaosong Hu, Fang Chen, Yuchen Zhu
Summary: A robust and sensitive isotope dilution UPLC-MS/MS method was developed for the simultaneous analysis of acrylamide, 5-hydroxymethylfurfural, and 2-amino-1 -methyl-6-phenylimidazo [4,5-b] -pyridine in thermally processed foods, demonstrating good separation and quantification accuracy.
Article
Food Science & Technology
Wei Wang, Xiaopu Ren, Yingjie Bao, Yuxia Zhu, Yawei Zhang, Junke Li, Zengqi Peng
Summary: The study shows that Zanthoxylum bungeanum Maxim. leaf extract and its polyphenols can effectively inhibit the formation of PhIP, with chlorogenic acid, hyperoside, and quercitrin significantly reducing the content of phenylacetaldehyde in the model systems. This process helps block the generation of PhIP by trapping phenylacetaldehyde and forming adducts.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Review
Chemistry, Medicinal
Jingshu Guo, Joseph S. Koopmeiners, Scott J. Walmsley, Peter W. Villalta, Lihua Yao, Paari Murugan, Resha Tejpaul, Christopher J. Weight, Robert J. Turesky
Summary: Well-done cooked red meat consumption is associated with aggressive prostate cancer risk. This study measured the levels of PhIP, a potential carcinogen found in cooked meat, in the hair of prostate cancer patients and analyzed the correlation with prostate pathology biomarkers and Gleason scores.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Engineering, Environmental
Hongmei Yang, Zhiwei Ji, Ru Wang, Daming Fan, Yueliang Zhao, Mingfu Wang
Summary: The study demonstrated that kappa-carrageenan, among eight tested hydrocolloids, exhibited significant inhibitory effects on the formation of PhIP, with a 90% reduction observed in fried beef patties. Further investigation suggested that kappa-carrageenan may inhibit PhIP formation by trapping creatinine and phenylacetaldehyde, which warrants additional study on the structures of adducts formed between these compounds.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Agriculture, Multidisciplinary
Xiuli Zhao, Hengchao Liu, Xiaofei Zhou, Xiaoxu Chen, Nan Hu, Yan Zhang, Shuo Wang
Summary: PhIP disrupts intestinal bacterial composition and inhibits lipid metabolism function in the intestinal flora. Furthermore, PhIP significantly inhibits the expression of gene sets associated with steroid hormone biosynthesis, fatty acid elongation, fatty acid degradation, and glycerolipid metabolism pathways in the colon.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Applied
Qi Wang, Weiwei Cheng, Yifeng Zhang, Qingzheng Kang, Vemana Gowd, Yuanyuan Ren, Feng Chen, Ka-Wing Cheng
Summary: Differential solvent extraction and phytochemical profiling were used to identify potent inhibitors of PhIP formation in Chinese chive. The inhibitory mechanism was found to be related to phenylacetaldehyde scavenging rather than antioxidant activities. Organic synthesis and structural elucidation confirmed the proposed mode of action for the novel inhibitor.
Article
Agriculture, Multidisciplinary
Yueliang Zhao, Hongmei Yang, Nana Zhang, Qian Zhou, Daming Fan, Mingfu Wang
Summary: The deacetylation degree of chitosan has a significant impact on heterocyclic aromatic amine formation, with 85% DD chitosan showing the strongest inhibitory effect on PhIP formation. In roast beef patties, the addition of 0.15% (w/w) 85% DD chitosan significantly reduced the formation of various heterocyclic aromatic amines and aldehydes, while potentially promoting fatty acid oxidation. Chitosan addition below 0.15% (w/w) did not have a significant effect on the texture of the roast samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Medjda Bellamri, Kyle Brandt, Kari Cammerrer, Tauqeerunnisa Syeda, Robert J. J. Turesky, Jason R. R. Cannon
Summary: Animal fat and iron-rich diets increase the risk of Parkinson's disease. Heterocyclic aromatic amines (HAAs), including harman and norharman, formed in cooked meats, suggest a connection between red meat consumption and PD.
CHEMICAL RESEARCH IN TOXICOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Peng Deng, Chaoyi Xue, Zhiyong He, Zhaojun Wang, Fang Qin, Emel Oz, Jie Chen, Aly Farag El Sheikha, Charalampos Proestos, Fatih Oz, Maomao Zeng
Summary: The key intermediates for PhIP generation were identified as benzaldehyde and phenylacetaldehyde. Histidine and proline showed inhibitory effects on PhIP formation in different model systems, and their combination at a low addition level significantly enhanced the suppression of PhIP formation by scavenging these key intermediates simultaneously.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Applied
Meilin Jing, Qingqing Jiang, Yamin Zhu, Daming Fan, Mingfu Wang, Yueliang Zhao
Summary: Acrolein increases the formation of PhIP by facilitating the degradation of phenylalanine and reacting with phenylalanine, creatinine, and PhIP. In addition, oxidation of the tilapia fish further increases the formation of PhIP in the roasted fish patties.
Article
Food Science & Technology
Lei Zhao, Na Zhou, Huimin Zhang, Fei Pan, Xin Ai, Yong Wang, Shuai Hao, Chengtao Wang
Summary: The study showed that cyanidin-3-O-glucoside (C3G) and its metabolite protocatechuic acid (PCA) can protect against cytotoxic effects induced by heterocyclic aromatic amines. They work by interfering with Nrf2 signaling and apoptotic cascade pathways to minimize cell damage. Additionally, C3G exhibited stronger protective effects compared to PCA.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Pharmacology & Pharmacy
Elsayed Salim, Alaa El-Din El-Sisi, Samia Sokar, Magda El-Sayad, Ethar Moussa
Summary: This study demonstrated that Metformin enhances the antitumor efficacy of doxorubicin and has beneficial effects on PhIP-induced mammary carcinogenesis by reducing tumor incidence and multiplicity, as well as inhibiting cellular proliferation and mRNA expression of ER alpha and EGF.
FUNDAMENTAL & CLINICAL PHARMACOLOGY
(2021)
Article
Toxicology
Hansruedi Glatt, Wolfram Engst, Simone Florian, Monika Schreiner, Chimgee Baasanjav-Gerber
Summary: Research has found that consuming Brassica vegetables can lead to the formation of DNA adducts in animal models. These adducts can be attributed to specific phytochemicals. Steaming the vegetables may result in higher levels of adducts in the large intestine due to inactivation of the enzyme responsible for activating the phytochemicals.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Genetics & Heredity
Abdulsamad Wafa, Belal Ali, Faten Moassass, Maged Kheder, Abdulmunim Aljapawe, Bassel Al-Halabi, Kristin Mrasek, Thomas Liehr, Walid Al-Achkar
Summary: This study investigated the CEBPA gene mutations in a Syrian family with AML. The results showed that all affected family members had a good response to chemotherapy and achieved complete remission. This research is important for understanding the relationship between CEBPA gene mutations and AML.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2022)
Article
Biology
Fernando H. S. de Souza, Francisco de M. C. Sassi, Pedro H. N. Ferreira, Luiz A. C. Bertollo, Tariq Ezaz, Thomas Liehr, Manolo F. Perez, Marcelo B. Cioffi
Summary: This study investigated the relationship between the evolution of multiple sex chromosomes together with allopatry and genetic diversity and speciation in the fish species Erythrinus erythrinus. The presence of multiple sex chromosomes was hypothesized to contribute to genetic differentiation and potentially accelerate speciation. The results showed high genetic differentiation among populations, with long-distance allopatry as the main agent of genetic divergence.
Editorial Material
Biochemistry & Molecular Biology
Milena Paneque, Thomas Liehr, Clara Serra Juhe, Ute Moog, Bela Melegh, Isabel Carreira
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Multidisciplinary Sciences
Felix L. Wascher, Nancy Stralis-Pavese, J. Mitchell McGrath, Britta Schulz, Heinz Himmelbauer, Juliane C. Dohm
Summary: The phylogeny of wild and cultivated beets is clarified using genomic analysis and it is found that Greece is the domestication site of sugar beet. This study provides comprehensive insights into the evolutionary relationships of beets.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Andre Weber, Thomas Liehr, Ahmed Al-Rikabi, Simal Bilgen, Uwe Heinrich, Jenny Schiller, Markus Stumm
Summary: This study reported a unique, complex, and discontinuous small supernumerary marker chromosome (sSMC) in a patient with multiple diseases.
Review
Geriatrics & Gerontology
Gilda Alves, Maria Helena Ornellas, Thomas Liehr
Summary: The CAMTA1 gene plays a central role in the human nervous system and is associated with brain tumors and neurological disorders. Mutations and variants of CAMTA1 are linked to neurodegenerative diseases, while single nucleotide polymorphisms are associated with memory problems and amyotrophic lateral sclerosis. However, further research is needed to understand the rearrangements and expression of CAMTA1 in different cases.
Article
Biochemistry & Molecular Biology
Gustavo A. Toma, Natalia dos Santos, Rodrigo dos Santos, Petr Rab, Rafael Kretschmer, Tariq Ezaz, Luiz A. C. Bertollo, Thomas Liehr, Fabio Porto-Foresti, Terumi Hatanaka, Alongklod Tanomtong, Ricardo Utsunomia, Marcelo B. Cioffi
Summary: The study investigated the karyotypes of different color phenotypes in Scleropages formosus using molecular cytogenetic techniques. The results revealed population genetic structure and microstructure differences in the karyotypes of the color phenotypes, but did not conclusively support the hypothesis of discrete lineages or evolutionary units among them.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dagmar Heydeck, Christoph Ufer, Kumar R. Kakularam, Michael Rothe, Thomas Liehr, Philippe Poulain, Hartmut Kuhn
Summary: The researchers created transgenic mice expressing human ALOX15 under the control of the aP2 promoter, which showed high expression in adipocytes, bone marrow cells, and peritoneal macrophages. The catalytic activity of the transgenic enzyme was confirmed through activity assays and plasma metabolite analysis. The aP2-ALOX15 mice exhibited gender-specific differences in body-weight kinetics compared to the wildtype controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Jhon A. D. Vidal, Francisco de M. C. Sassi, Renata L. R. de Moraes, Roberto F. Artoni, Thomas Liehr, Marcelo B. Cioffi, Mara C. de Almeida
Summary: In this study, the similarity of X and Y chromosomes in four Omophoita species was examined, and genomic differentiation was compared to better understand the evolutionary process of the giant sex chromosomes. The results showed significant genomic similarity between the sexes and a sex-specific region on the Y chromosome, as well as genomic divergence between species. However, whole chromosome painting experiments revealed high intra- and interspecific similarity of the sex chromosomes in O. octoguttata. Our data support a common origin and canonical evolution of the sex chromosomes in this group, as they have high genomic similarity between them.
Review
Biochemistry & Molecular Biology
Galina Hovhannisyan, Tigran Harutyunyan, Rouben Aroutiounian, Thomas Liehr
Summary: Cell-free DNA (cfDNA) has become a commonly used diagnostic marker in various pathologies, including viral infections like COVID-19, due to its higher sensitivity compared to standard biochemical parameters. This review focuses on the identification, sources, molecular features, and mechanisms of release and clearance of cfDNA. It discusses the general suitability of cfDNA for diagnostic purposes, potential limitations, and future directions, with a special emphasis on coronavirus infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Francisco de Menezes Cavalcante Sassi, Alexandr Sember, Geize Aparecida Deon, Thomas Liehr, Niklas Padutsch, Osvaldo Takeshi Oyakawa, Marcelo Ricardo Vicari, Luiz Antonio Carlos Bertollo, Orlando Moreira-Filho, Marcelo de Bello Cioffi
Summary: The study investigates the chromosomal rearrangements and associated synteny blocks in the origin of a multiple X1X2Y sex chromosome system in the Neotropical catfish genus Harttia. The results suggest that the X1X2Y sex chromosome systems in H. punctata, H. duriventris and H. villasboas represent the same linkage groups which also form the putative XY sex chromosomes of H. rondoni. The study also indicates that the X1X2Y sex chromosome system may have formed through an X chromosome fission rather than a previously proposed Y-autosome fusion.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Mariannah Pravatti Barcellos de Oliveira, Rafael Kretschmer, Geize Aparecida Deon, Gustavo Akira Toma, Tariq Ezaz, Caio Augusto Gomes Goes, Fabio Porto-Foresti, Thomas Liehr, Ricardo Utsunomia, Marcelo de Bello Cioffi
Summary: In this study, the dynamics of satellite DNAs (SatDNAs) accumulation and elimination during W chromosome differentiation in Triportheus fish were investigated. The majority of repetitive sequences were eliminated as speciation occurred in five additional Triportheus species. While the W chromosomes continued to degenerate, the Z chromosomes in some species began to accumulate TauSatDNAs. Species-specific SatDNAs were likely amplified in the heterochromatic regions of both Z and W chromosomes in each species. Thus, the W chromosomes of various Triportheus species underwent significant evolutionary changes within a short period of time (15-25 Myr) after divergence.
Article
Biochemistry & Molecular Biology
Renata Luiza Rosa de Moraes, Francisco de Menezes Cavalcante Sassi, Jhon Alex Dziechciarz Vidal, Caio Augusto Gomes Goes, Rodrigo Zeni dos Santos, Jose Henrique Forte Stornioli, Fabio Porto-Foresti, Thomas Liehr, Ricardo Utsunomia, Marcelo de Bello Cioffi
Summary: Chromosomal rearrangements play a significant role in the evolution of fish genomes, and satellite DNA sequences have potential relevance in both sex and karyotype evolution. This study investigated the association of satDNAs with chromosome-shuffling events and their involvement in the evolution of sex chromosomes in Pyrrhulina fish. In addition, a large collection of satDNAs for both species was characterized for the first time, and their potential involvement in karyotype evolution was demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Geize Aparecida Deon, Larissa Glugoski, Terumi Hatanaka, Francisco de Menezes Cavalcante Sassi, Viviane Nogaroto, Luiz Antonio Carlos Bertollo, Thomas Liehr, Ahmed Al-Rikabi, Orlando Moreira Filho, Marcelo de Bello Cioffi, Marcelo Ricardo Vicari
Summary: This study investigates the chromosomal rearrangements in the Neotropical armored catfish genus Harttia using powerful tools such as comparative whole chromosome painting. The results reveal the importance of translocation events in the karyotype evolution of Harttia species, as well as the independent origin of two types of multiple sex chromosomes.
GENETICS AND MOLECULAR BIOLOGY
(2022)