Journal
CARBOHYDRATE RESEARCH
Volume 355, Issue -, Pages 56-62Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2012.04.004
Keywords
Edwardsiella tarda; Lipopolysaccharide; O-Antigen; Bacterial polysaccharide structure; Abequose; 2-Amino-2-deoxyglycerol
Funding
- Federal Targeted Program for Research and Development in Priority Areas of Russia's Science and Technology Complex [16.552.11.7050]
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Lipopolysaccharides of four strains of Edwardsiella tarda were degraded by mild acid hydrolysis, and the released O-polysaccharides were isolated by GPC and studied by sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, including 2D H-1, H-1 COSY, TOCSY, ROESY, H-1, C-13 HMBC, HSQC and HSQC-TOCSY experiments. The O-polysaccharide from E. tarda PCM 1153 was found to contain D-GalA, D-GlcNAc, D-Gal and 2-amino-1,3-propanediol (GroN). In the tetrasaccharide repeating unit, GroN is amide-linked to one of the GalA residues, and Gal is non-stoichiometrically 2-or 3-O-acetylated (similar to 45% at each position): [GRAPHICS] Three other E. tarda strains examined (PCM 1145, PCM 1151 and PCM 1158) share the following O-polysaccharide structure: [GRAPHICS] where Abe indicates 3,6-dideoxy-D-xylo-hexose (abequose). This structure resembles those of Citrobacter freundii O22 (PCM 1555) and Salmonella enterica O4. In accordance with the structural data, SDS-PAGE and immunoblotting of the lipopolysaccharides with anti-C. freundii O22 serum demonstrated that the O-antigens of the three E. tarda strains are serologically identical to each other and to the O-antigens of C. freundii O22 and S. enterica O4. (C) 2012 Elsevier Ltd. All rights reserved.
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