Structural similarities of fucoidans from brown algae Silvetia babingtonii and Fucus evanescens, determined by tandem MALDI-TOF mass spectrometry

Rapid mass spectrometric investigation of oligosaccharides, obtained by autohydrolysis of fucoidans from brown algae Silvetia babingtonii and Fucus evanescens (Fucales, Phaeophyceae) has shown both similarities and differences in structural features/sulfation pattern of their fragments, obtained in the same conditions. Tandem MALDI-TOF MS of fucooligosaccharides with even DP (degree of polymerization) was close to that observed for fucoidan from F. evanescens. Slight differences in tandem mass spectra of fragments with odd DP indicated, probably, sulfation at C-3 (instead of C-2 in F. evanescens) of some (1 -> 4)-linked alpha-L-Fucp residues and/or the presence of short blocks, built up of (1 -> 3)-linked alpha-L-Fucp residues. (C) 2012 Elsevier Ltd. All rights reserved.