Article
Microbiology
Ayako Horigome, Nanami Hashikura, Keisuke Yoshida, Jin-zhong Xiao, Toshitaka Odamaki
Summary: This study investigates the impact of the intrinsic gut microbiota on the prebiotic effects of 2'-fucosyllactose (2'-FL), particularly on Blautia proliferation, and identifies the role of lactose and fucose as substrates mediating the growth of Blautia. The findings highlight the importance of individualized nutritional approaches in controlling the gut microbiota.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Mohamad Eshaghi Gorji, Malcolm Turk Hsern Tan, Dan Li
Summary: This study found that Bifidobacterium bifidum JCM 1254 treatment significantly increased the accumulation of human noroviruses in oyster digestive tissue and enhanced the HBGA antigenicity, possibly due to the substrate specificity of the fucosidases produced by B. bifidum JCM 1254. This suggests an indirect interaction between bacterial microbiota and human NoVs, providing a potential strategy for mitigating NoV contamination from shellfish by obtaining bacterial strains with specified fucosidase production in the future.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Sarah Barelier, Gerlind Sulzenbacher
Summary: In this study, Armstrong and his team investigate the structure of human fucosidase FucA1. Their findings not only resolve the debate on the enzyme's catalytic mechanism but also offer a valuable structural template for the design of drugs to treat fucosidosis, a rare but severe lysosomal storage disease.
Article
Chemistry, Organic
Debora Pratesi, Stefania Mirabella, Giulia Petrucci, Camilla Matassini, Cristina Faggi, Francesca Cardona, Andrea Goti
Summary: Glycosylamine derivatives, which are biologically relevant compounds, can be synthesized by utilizing the allyl cyanate to isocyanate rearrangement of carbamate-substituted glycals, followed by nucleophilic addition with alcohols or amines. The reaction proceeds through a [3,3]-sigmatropic pericyclic mechanism and exhibits stereospecificity. The synthesized unsaturated N-glycosides can be selectively cis-dihydroxylated to obtain different 1-aminosugars.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Alice D. Baudot, Victoria M-Y Wang, Josh D. Leach, Jim O'Prey, Jaclyn S. Long, Viola Paulus-Hock, Sergio Lilla, David M. Thomson, John Greenhorn, Farah Ghaffar, Colin Nixon, Miep H. Helfrich, Douglas Strathdee, Judith Pratt, Francesco Marchesi, Sara Zanivan, Kevin M. Ryan
Summary: This study reveals the importance of glycan degradation in macroautophagy. The absence of the glycosidase FUCA1 leads to lysosomal glycan accumulation and impaired autophagic flux. Dysregulated glycan degradation hinders autophagosome-lysosome fusion and contributes to the pathology of fucosidosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Organic
Mengbi Guo, Xin Wang, Yitong Wang, Zhuang Hou, Chun Guo, Ping Gong
Summary: A facile and efficient method for synthesizing C1-aminosugar derivatives under catalyst-free conditions is described. The method utilizes readily available benzoyl glycosyl bromides to smoothly obtain a broad scope of C1-aminosugar derivatives with good yields. It offers the advantages of short reaction time, high yield, and a wide substrate scope.
TETRAHEDRON LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Ting Li, Bingbing Xu, Dengxian Fu, Qian Wan, Jing Zeng
Summary: In this study, we propose a mild strategy for deprotecting cyclic sulfamidates to access amino sugars using the Kukhtin-Ramirez reaction. The sulfonic group of cyclic sulfamidates is removed through an N-H insertion reaction involving the Kukhtin-Ramirez adducts, followed by a base-promoted reductive N-S bond cleavage. The protocol's mild reaction conditions allow for the formation of amino alcohols with multiple functional groups.
Article
Biochemistry & Molecular Biology
Xiaohua Liu, Xin Geng, Weizhi Liu, Qianqian Lyu
Summary: This study identified and characterized an alpha-L-fucosidase from the GH29 family, named PsaFuc. PsaFuc exhibited temperature sensitivity and its activity could be prolonged by adding Ca2+. The presence of Ca2+ improved the overall structural stability of PsaFuc and induced a new active conformation that was relatively more stable at lower temperatures.
PROCESS BIOCHEMISTRY
(2022)
Review
Oncology
Jinxing Fu, Qing Guo, Yuan Feng, Peng Cheng, Anhua Wu
Summary: Glycosidases and glycosyltransferases play essential roles in tumor development by influencing cell interactions and signaling pathways. Alpha-L-fucosidases (AFUs), a member of glycoside hydrolase (GH) families 29A, hydrolyze fucose residues and affect crucial cell-extracellular matrix interactions. In humans, there are two isoforms of AFUs, FUCA1 and FUCA2, which are involved in various diseases and tumor types. AFUs have the potential to be therapeutic targets based on their involvement in signaling pathways and tumor progression.
Article
Oncology
Yi Xiao, Xiaoyu Jiang, Ke Yin, Tianshu Miao, Hanlin Lu, Wenqing Wang, Lijuan Ma, Yinghui Zhao, Chunyan Liu, Yun Qiao, Pengju Zhang
Summary: The under-characterized deubiquitinase USP35 is found to be overexpressed in colorectal cancer (CRC) and promotes cell proliferation and chemotherapy resistance. The study identifies alpha-L-fucosidase 1 (FUCA1) as a direct deubiquitination target of USP35 and demonstrates its essential role in CRC cell proliferation and chemo-resistance. The USP35-FUCA1 axis may contribute to platinum resistance in CRC by up-regulating nucleotide excision repair (NER) components.
Article
Biochemistry & Molecular Biology
Ziyu Zhang, Yuting Li, Mujunqi Wu, Zhen Gao, Bin Wu, Bingfang He
Summary: In this study, a soil-oriented α-L-fucosidase-producing strain was isolated and identified as Enterococcus gallinarum ZS1. Escherichia coli was used to clone and express the α-L-fucosidase gene (entfuc). The enzyme EntFuc was found to have a high conversion rate and is a promising enzyme source for the biosynthesis of the important nutrient 2'-fucosyllactose (2'FL) in human milk.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Federica De Lise, Andrea Strazzulli, Roberta Iacono, Nicola Curci, Mauro Di Fenza, Luisa Maurelli, Marco Moracci, Beatrice Cobucci-Ponzano
Summary: Genetic code decoding is no longer considered universal and immutable, but rather flexible. In Archaea, translational recoding has been extensively studied to regulate the decoding of selenocysteine and pyrrolysine, but further events in other species remain to be explored. The study of recoding episodes in Archaea is urgently needed.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Rikako Shishiuchi, Hyejin Kang, Takayoshi Tagami, Yoshitaka Ueda, Weeranuch Lang, Atsuo Kimura, Masayuki Okuyama
Summary: This study identified two α-L-glucosidases derived from Cecernbia lonarensis LW9 and confirmed the existence of α-L-glucosides in nature. These enzymes selectively hydrolyze α-L-glucosides and can also synthesize α-L-glucosidic linkages through transglucosylation activity. This research will contribute to further investigation on the physiological effects of α-L-glucosides in humans and the discovery of novel α-L-glucoside-related enzymes.
Article
Chemistry, Medicinal
Jonathan Cotton, Chris Marc Goehring, Anna Kuehn, Andreas Maurer, Kerstin Fuchs, Bernd J. Pichler
Summary: Researchers have developed a novel PET tracer to image AF in vivo and plan to apply it to relevant disease models. In vitro evaluations showed significantly higher uptake of the new tracer in tumor cells compared to controls.
Article
Chemistry, Multidisciplinary
Daniele Polidoro, Alvise Perosa, Elena Barbaro, Matteo Feltracco, Elena Argiriadis, Maurizio Selva
Summary: A multiphase system composed of two immiscible liquids and a catalyst was designed and investigated for the chemoselective catalytic hydrogenation of biobased sugars and amino/amido-sugars. The study showed that this method can improve product isolation and catalyst recycling, as well as prevent metal contamination.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
