Preparation and evaluation of lactose-modified monoliths for the adsorption and decontamination of plant toxins and lectins

A series of sugar-modified porous silica monoliths with different sugar ligands (beta-lactoside, beta-N-acetyllactosaminide, beta-D-galactoside, beta-D-N-acetylgalactosaminide and beta-D-glucoside) and linkers were prepared and evaluated using plant toxins and lectins including ricin and a Ricinus communis agglutinin (RCA(120)). Among these sugar monoliths, a lactose monolith carrying a triethylene glycol spacer adsorbed ricin and RCA(120) with the highest efficiency. The monolith showed no binding with albumin, globulin, and lectins from Jack beans, Osage orange, Amur maackia and wheat germ. All these data support the utility of the lactose-modified monolith as a tool for adsorption and decontamination of plant toxins. (C) 2011 Elsevier Ltd. All rights reserved.