Article
Food Science & Technology
Maxime Haure, Thi Kim Chi Nguyen, Aurelie Cendres, Sandrine Perino, Yves Wache, Helene Licandro
Summary: Two Bacillus isolates were identified to have glycosidase activities on rutin and grape aroma precursors, releasing monoterpenoids and benzenoids, especially with significant effects in grape pomace. These glycosidases could be valuable tools for targeting rutinosidic glycosides in grape and enhancing the value of grape pomace.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Annarita Panighel, Mirko De Rosso, Antonio Raffaele Mazzei, Michele Fugaro, Fabiola De Marchi, Riccardo Flamini
Summary: Liquid chromatography/high-resolution mass spectrometry (LC/HRMS) is used for identifying grape metabolites as variety markers. LC/HRMS analysis of three white grape juices identified 70 putative glycosidic terpenols, norisoprenoids, and benzenoids. Each grape variety also had specific markers, such as Pinot grigio having four signals exclusive to it. These variety markers can be used to detect unauthorized grape varieties and practices in white wine production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Food Science & Technology
Cristina Cebrian-Tarancon, Jose Oliva, Miguel Angel Camara, Gonzalo L. Alonso, M. Rosario Salinas
Summary: This study introduces a method to analyze intact glycosidic aroma precursors in grapes using HPLC-DAD, a simple and inexpensive analytical technique. A total of 20 compounds were selected and identified using HPLC-qTOF-MS. The most abundant group observed were disaccharides hexose pentose and the main aglycones found were monoterpendiols.
Article
Chemistry, Analytical
Desen Su, Yunyun Zheng, Ziqiang Chen, Yuwu Chi
Summary: An UPLC-MS/MS method was established for the simultaneous determination of six glycosidic aroma precursors in pomelo, showing advantages of rapidity, high sensitivity, and good accuracy. The study found that more glycosidic aroma precursors were present in flowers compared to leaves or fruits in pomelo plants. This method holds promising application in qualifying and quantifying these six glycosidic aroma precursors in pomelo.
Article
Food Science & Technology
Mirko De Rosso, Valentina Lonzarich, Luciano Navarini, Riccardo Flamini
Summary: Grape aroma precursors have been extensively studied and identified, but the investigation of these compounds in green Coffea arabica is limited. This study targeted the investigation of glycoside precursors in green C. arabica from different geographical origins, using knowledge from grape and a high-resolution mass spectrometry database. The results revealed the presence of high linalool hexose-pentose and different glycosides in the investigated samples, as well as the identification of putative norisoprenoid glycosides. The findings also suggest the possible formation of 13-damascenone by hydrolysis and dehydration of a putative 3-hydroxy-13-damascone glycoside precursor identified for the first time in coffee. The identification of these glycosides contributes to understanding the coffee biology.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Giacomo Morreale, Tyrone Possamai, Annarita Panighel, Mirko De Rosso, Lorenzo Lovat, Riccardo Flamini, Daniele Migliaro
Summary: This study reports the discovery and characterization of 'Glera rosa', a mutant grape variety with a different berry skin color compared to the main white cultivar used for Prosecco wine production. Genetic and chemical analyses revealed the relationship between the mutation and the genes involved in polyphenol biosynthesis, as well as the changes in metabolites in the mutant grape.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Environmental Sciences
Tanzina Akther, Bernhard Rappenglueck, Olabosipo Osibanjo, Armando Retama, Olivia Rivera-Hernandez
Summary: This study conducted measurements of volatile organic compounds (VOCs), inorganic pollutants, surface meteorological data, and the Planetary Boundary Layer height (PBLH) in an urban site in Mexico City. Positive Matrix Factorization (PMF) identified four emission source factors of VOCs, equivalent black carbon (eBC), gaseous pollutants (CO, NO, NO2, SO2, NH3), and ions (Na+, Mg2+, Ca2+, NO3-, NH4+). Isoprene and ethylene were found to be the highest oxidant and O-3 forming species. The data normalized to the PBLH variation revealed continued production of O-3 precursors in the afternoon, especially during a strong O-3 episode.
Article
Chemistry, Physical
Tao Dong, Qian Huang, Lu Chen, Jiahui Peng, Hui Hu, Qingming Huang, Xiaohui Chen
Summary: Au particles supported on TS-1 were prepared using ammonium salt solution as a promoter. The results showed that this method did not destroy the structure of TS-1 and retained the tetra-coordination skeleton of titanium. The gold precursor underwent a series of reactions to form gold nanoparticles.
CHEMICAL PHYSICS LETTERS
(2022)
Article
Chemistry, Applied
Daris Grizic, Alf Lamprecht
Summary: This study aimed to understand the impact of process parameters on celecoxib-loaded microsphere preparation. Results showed that microspheres had rough walls, sizes ranging from 21 μm to 122 μm, and encapsulation efficiencies between 28.3% and 66.8%. Release rates were influenced by polymer concentration, emulsion phase ratio, and residual propylene carbonate content.
JOURNAL OF MICROENCAPSULATION
(2021)
Article
Biochemical Research Methods
Shuba Krishnan, Hampus Nordqvist, Anoop T. Ambikan, Soham Gupta, Maike Sperk, Sara Svensson-Akusjarvi, Flora Mikaeloff, Rui Benfeitas, Elisa Saccon, Sivasankaran Munusamy Ponnan, Jimmy Esneider Rodriguez, Negin Nikouyan, Amani Odeh, Gustaf Ahlen, Muhammad Asghar, Matti Sallberg, Jan Vesterbacka, Piotr Nowak, Akos Vegvari, Anders Sonnerborg, Carl Johan Treutiger, Ujjwal Neogi
Summary: This study used patient-derived data and in vitro experiments to investigate the relationship between metabolic pathways and the replication of the SARS-CoV-2 virus, finding that severe disease is characterized by increased plasma glucose levels. Immune phenotype analysis revealed altered expression patterns, and glycolysis and glutaminolysis were identified as essential for virus replication.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Chemistry, Multidisciplinary
Shuang Yu, Jie Meng, Qinying Pan, Qian Zhao, Tonu Pullerits, Yingguo Yang, Kaibo Zheng, Ziqi Liang
Summary: In this paper, highly ordered lattice arrangements in 2D lead halide perovskites were achieved using the 4,5-dicyanoimidazole (DCI) additive. Through a series of characterization methods, the epitaxial growth of multi-phases was revealed, which effectively reduced trap density and prolonged carrier lifetime.
ENERGY & ENVIRONMENTAL SCIENCE
(2022)
Review
Immunology
Wei Zhang, Hao Cheng, Yuanyuan Gui, Qipeng Zhan, Si Li, Wenliang Qiao, Aiping Tong
Summary: This review explores the therapeutic functions of mannose in inflammatory diseases, highlighting its ability to suppress inflammation by inducing regulatory T cells, inhibiting effector T cells, and modulating the gut microbiome for anti-inflammatory effects. The findings suggest that mannose treatment is a safe and promising strategy for suppressing inflammatory diseases, including autoimmune and allergic conditions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ummey Jannatun Nahar, Istvan Toth, Mariusz Skwarczynski
Summary: This article discusses the use of adjuvants and vaccine delivery systems to enhance vaccine efficacy. It focuses on the role of mannose receptors in antigen recognition by antigen presenting cells (APCs). The production strategies and effectiveness of mannosylated vaccines are examined, considering factors such as mannose valency, density, and routes of administration. The advantages, disadvantages, and future directions of mannosylation strategy are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biochemical Research Methods
Mirko De Rosso, Annarita Panighel, Daniele Migliaro, Tyrone Possamai, Fabiola De Marchi, Riccardo Velasco, Riccardo Flamini
Summary: A breeding program was conducted to produce new grape varieties tolerant to fungal pathogens by crossing Vitis vinifera cv. Glera with resistant genotypes. Resistance gene-based markers analysis identified five genotypes with inherited resistance loci against mildews. Metabolomic profiling of grape glycosidic derivatives using UHPLC/QTOF mass spectrometry and database identification revealed distinct profiles in original resistant genotypes and varying similarity degrees in crossings to V. vinifera parent. The Glera x Bronner and Glera x Solaris crossings were found to be more suitable for producing high-sustainable Prosecco wines. Coupling glycosidic volatile precursors profiling with multivariate statistical analysis can effectively characterize grapes and evaluate their enological potential.
JOURNAL OF MASS SPECTROMETRY
(2023)
Review
Biochemistry & Molecular Biology
Fiona E. Freeman, Ross Burdis, Daniel J. Kelly
Summary: Regenerating large bone defects is a clinical challenge that has sparked interest in additive manufacturing and 3D bioprinting for superior bone graft substitutes. Two main approaches to bioprinting implants exist, with one allowing direct implantation and the other requiring in vitro culture for tissue maturation before implantation. This review explores the concepts, challenges, and applications of both strategies for bone regeneration.
TRENDS IN MOLECULAR MEDICINE
(2021)
Article
Oncology
Annette G. Beck-Sickinger, Daniel P. Becker, Oksana Chepurna, Bhaskar Das, Sebastian Flieger, Evamarie Hey-Hawkins, Narayan Hosmane, Satish S. Jalisatgi, Hiroyuki Nakamura, Rameshwar Patil, Maria da Graca H. Vicente, Clara Vinas
Summary: This article summarizes current research on the development of boron delivery drugs for boron neutron capture therapy, which were presented and discussed at the National Cancer Institute (NCI) Workshop on Neutron Capture Therapy held on April 20-22, 2022. The most commonly used boron sources are icosahedral boron clusters attached to various molecules such as peptides, proteins, porphyrin derivatives, dendrimers, polymers, and nanoparticles, as well as encapsulated into liposomes. These boron clusters and/or carriers can be labeled with contrast agents, allowing for imaging techniques like PET, SPECT, and fluorescence to quantify tumor-localized boron and serve as theranostic agents.
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
(2023)
Article
Pharmacology & Pharmacy
Sebastian Braun, Svetlana Paskas, Markus Laube, Sven George, Bettina Hofmann, Peter Loennecke, Dieter Steinhilber, Jens Pietzsch, Sanja S. Mijatovic, Danijela Maksimovic-Ivanic, Evamarie Hey-Hawkins
Summary: In this study, carborane-containing dual COX-2/5-LO inhibitors were designed by incorporating metabolically stable, sterically demanding, and hydrophobic carboranes into existing inhibitors. Five carborane-containing derivatives showed high inhibitory activities towards COX-2 and 5-LO, with meta-carborane derivative 3 demonstrating higher anticancer activity compared to RWJ-63556. The accumulation of lipid droplets in cells indicated the blockage of COX-2 and 5-LO pathways, suggesting a promising approach for the design of potent dual COX-2/5-LO inhibitors.
ADVANCED THERAPEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Kyzgaldak Ramazanova, Peter Loennecke, Evamarie Hey-Hawkins
Summary: The reactive P-N bond in a racemic mixture of endo-1-phospha-2-azanorbornene (PAN) is cleaved with enantiomerically pure lithium alkoxides and protonation to produce diastereomeric mixtures of P-chiral 1-alkoxy-2,3-dihydrophosphole derivatives. The isolation of these compounds is challenging due to the reversibility of the reaction, but methylation and sulfur protection prevent elimination reaction. The resulting P-chiral diastereomeric 1-alkoxy-2,3-dihydrophosphole sulfide mixtures can be easily separated and characterized, and can be reduced to phosphorus(III) P-stereogenic 1-alkoxy-2,3-dihydrophospholes for use in asymmetric homogeneous transition metal catalysis.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Medicinal
Sebastian Braun, Svetlana Paskas, Markus Laube, Sven George, Bettina Hofmann, Peter Loennecke, Dieter Steinhilber, Jens Pietzsch, Sanja Mijatovic, Danijela Maksimovic-Ivanic, Evamarie Hey-Hawkins
Summary: The presence of inflammatory mediators in the tumor microenvironment indicates cancer-related inflammatory processes, and targeting these mediators and related signal pathways may provide a rational strategy for cancer treatment. This study focuses on incorporating carboranes into dual COX-2/5-LO inhibitors, key enzymes in eicosanoid biosynthesis. The results show that carborane-based tebufelone analogs exhibit no COX inhibition but 5-LO inhibitory activity, and structural modifications enhance their anticancer activity. Thus, this strategy is promising for designing potent 5-LO inhibitors with potential application as cytostatic agents.
Article
Chemistry, Medicinal
Philipp Stockmann, Lydia Kuhnert, Lisa Zoerner, Walther Honscha, Evamarie Hey-Hawkins
Summary: The role of ATP-binding cassette (ABC) transporter-mediated multidrug resistance (MDR) in anti-cancer therapy is challenging, as cancer cells exploit efflux transporters like BCRP to secrete chemotherapeutic substances. In this study, a hybrid BCRP inhibitor was designed by combining a prominent scaffold N-phenylquinazolin-4-amine with a boron-carbon cluster. The resulting compound showed increased inhibitory activity towards human ABCG2 and the ability to reverse drug resistance.
Review
Biochemistry & Molecular Biology
Zeno Fickenscher, Evamarie Hey-Hawkins
Summary: Inspired by the role of multimetallic assemblies in enzyme catalysis, a wide range of heterobimetallic complexes have been developed for use in homogeneous catalysis. These complexes, starting from simple ones with s-donating and p-accepting ligands, have evolved into more complex systems over the past two decades. The presence of a second metal center in the active catalyst allows for new reaction pathways and significantly increased catalytic activity. This review focuses on the mechanistic aspects of heterobimetallic complexes in homogeneous catalysis, showcasing their versatility and the different types of interactions and reactions they enable.
Article
Biochemistry & Molecular Biology
Zeno B. G. Fickenscher, Peter Loennecke, Anna K. Mueller, Oldamur Holloczki, Barbara Kirchner, Evamarie Hey-Hawkins
Summary: Two heterometallic Mo,M' complexes (M' = Ir-III, Rh-III) were synthesized and characterized. They showed a significant synergistic effect, especially Mo and Ir, leading to a fourfold increase in catalytic activity compared to a binary mixture of the monometallic counterparts. The mechanism of CO2 hydrogenation to formate by a monometallic Ir-III catalyst was studied, revealing the importance of the metal-base adduct and inner-sphere hydride transfer.
Article
Biochemistry & Molecular Biology
Sebastian Braun, Sanja Jelaca, Markus Laube, Sven George, Bettina Hofmann, Peter Loennecke, Dieter Steinhilber, Jens Pietzsch, Sanja Mijatovic, Danijela Maksimovic-Ivanic, Evamarie Hey-Hawkins
Summary: Targeting inflammatory mediators and related pathways is a rational strategy for cancer treatment. Incorporating carboranes into dual COX-2/5-LO inhibitors shows promise, with compounds exhibiting high 5-LO inhibitory activities. However, carborane analogs demonstrate lower anticancer activity compared to related compounds in cell viability studies. R-830-Cb, a carborane-based analog, shows potential for further mechanistic and in vivo studies due to the advantages of boron cluster incorporation.
Article
Pharmacology & Pharmacy
Liridona Useini, Teodora Komazec, Markus Laube, Peter Loennecke, Jonas Schaedlich, Sanja Mijatovic, Danijela Maksimovic-Ivanic, Jens Pietzsch, Evamarie Hey-Hawkins
Summary: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for pain, fever, and inflammation, and have reported antitumor properties. Selectivity for the COX-2 isoform of NSAIDs is of high interest to reduce off-target effects. Modified NSAIDs using carborane clusters show altered selectivity profile and isonimesulide and its carborane derivatives exhibit stronger antitumor potential compared to nimesulide and isonimesulide. Additionally, replacement of the phenyl ring with a carborane moiety shifts the COX activity from nonactive to active compounds.
ADVANCED THERAPEUTICS
(2023)
Article
Pharmacology & Pharmacy
Ivana Predarska, Mohamad Saoud, Valentin Cepus, Evamarie Hey-Hawkins, Goran N. Kaluderovic
Summary: This article investigates the effects of oxaliplatin and its derivatives on different tumor types, and designs platinum(IV) platforms based on oxaliplatin to assess their antitumor capacity against various breast cancer cell lines.
ADVANCED THERAPEUTICS
(2023)
Article
Chemistry, Physical
Nils Koenig, Yokari Godinez-Loyola, Hendrik Weiske, Sergej Naumov, Peter Loennecke, Ralf Tonner-Zech, Cristian A. Strassert, Evamarie Hey-Hawkins
Summary: In this study, a facile synthetic strategy for obtaining propeller-rich molecules bearing a thieno[3,2-b]phosphole motif was reported via MnIII-mediated oxidative annulation reaction. All synthesized compounds showed aggregation-induced emission (AIE) characteristics, strong fluorescence in the solid state, and the photophysical properties were dependent on the substituents. The phosphinated thieno[3,2-b]phospholes exhibited a high degree of restrictions on intramolecular motions, leading to powerful solid-state light emitters with fluorescence quantum yields close to 100% and lifetimes up to 7.9 ns, even at room temperature.
CHEMISTRY OF MATERIALS
(2023)
Editorial Material
Chemistry, Multidisciplinary
Kyzgaldak Ramazanova, Peter Loennecke, Evamarie Hey-Hawkins
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Oncology
Lydia Kuhnert, Robert Kuhnert, Menyhart B. Sarosi, Cathleen Lakoma, Birte K. Scholz, Peter Loennecke, Evamarie Hey-Hawkins, Walther Honscha
Summary: Chemotherapy is often hindered by multidrug resistance, and the inhibition of ABCG2 efflux transporter shows promise in overcoming drug resistance. This study successfully enhanced the affinity of baicalein towards ABCG2 by replacing the phenyl ring with a meta-carborane and adding three methoxy substituents. The modified compound demonstrated significant inhibitory effects on ABCG2-mediated drug resistance and holds potential for the development of efficient ABCG2 inhibitors.
MOLECULAR ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kyzgaldak Ramazanova, Soumyadeep Chakrabortty, Fabian Kallmeier, Nadja Kretzschmar, Sergey Tin, Peter Loennecke, Johannes G. de Vries, Evamarie Hey-Hawkins
Summary: Sulfur-protected enantiopure P-chiral 1-phosphanorbornane silyl ethers 5a,b were obtained in high yields via reaction with tert-butyldimethylsilyl chloride (TBDMSCl) and triphenylsilyl chloride (TPSCl), and were subsequently purified and characterized. The bulky monodentate enantiopure phosphorus(III) 1-phosphanorbornane silyl ethers 6a,b were obtained by desulfurization with excess Raney nickel, and showed better activity and selectivity as ligands in iridium-catalyzed asymmetric hydrogenation.
Article
Biochemistry & Molecular Biology
Kyzgaldak Ramazanova, Anna Karina Mueller, Peter Loennecke, Oldamur Holloczki, Barbara Kirchner, Evamarie Hey-Hawkins
Summary: The reactive P-N bond can be cleaved by simple alcohols in 1-phospha-2-azanorbornenes, resulting in a racemic mixture of P-chiral 2,3-dihydrophosphole derivatives. The reversibility of the reaction prevents the isolation of the products, but sulfurization of the phosphorus atom can block the reaction and yield structurally characterized compounds.
