Article
Biochemistry & Molecular Biology
Carlotta Ciaramelli, Alessandro Palmioli, Maura Brioschi, Simona Viglio, Maura D'Amato, Paolo Iadarola, Solveig Tosi, Laura Zucconi, Cristina Airoldi
Summary: In Antarctica, ice-free areas harbor microorganisms adapted to harsh conditions. This study presents an efficient protocol for extracting and metabolically profiling Antarctic soils using NMR spectroscopy and mass spectrometry. The results demonstrate the complementarity of the two techniques and their suitability for analyzing underexplored samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ilona Krabicova, Bohumil Dolensky, Michal Rezanka
Summary: Mannosylated structures have been studied in biomedical applications due to their ability to bind with specific biological receptors. The azide-alkyne click reaction is a common method used to link a functional moiety to a structure. In order to maintain its bioactivity, it is necessary to prepare and isolate a high-purity propargylated mannose derivative. Three known methods of preparing propargyl-alpha-mannopyranoside were revisited, and the products were analyzed using NMR spectroscopy. The experiments showed that one-step procedures did not yield pure propargyl-alpha-mannopyranoside, while a three-step procedure produced the desired compound with high purity.
Article
Biochemical Research Methods
Sarah Schleicher, Dominik Horoba, Philip Krafzig, Petra Mischnick
Summary: In this study, the hydroxyethyl substitution in cellulose ethers was analyzed using ESI-IT-MS. The correct quantification of the constituents was achieved through the use of internal standards and optimization of measurement conditions. The study also highlighted potential errors in relative quantification when using internal standards with similar chemistry or for analytes with related structures.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Donatella Aiello, Massimiliano Cordaro, Anna Napoli, Claudia Foti, Ottavia Giuffre
Summary: This study investigated the acid-base behavior and complexation properties of biomembrane headgroup compounds with Mg2+ under different conditions. The results showed the formation of different species for the compounds at various temperatures and ionic strengths.
FRONTIERS IN CHEMISTRY
(2022)
Article
Polymer Science
Olga Trhlikova, Vera Vlckova, Sabina Abbrent, Katerina Valesova, Livia Kanizsova, Katerina Sklenickova, Aleksandra Paruzel, Sonia Bujok, Zuzana Walterova, Petra Innemanova, Martin Halecky, Hynek Benes
Summary: The study found that fully aliphatic PUR foam exhibits significant degradability in microbial degradation, with good biodegradation performance in soil. These results demonstrate that a biologically active PUR foam can be successfully prepared for applications requiring material bioactivity such as biorecycling.
POLYMER DEGRADATION AND STABILITY
(2021)
Article
Materials Science, Paper & Wood
Yuko Yoneda, Toshinari Kawada, Markus Bacher, Alexander Prado-Roller, Hubert Hettegger, Antje Potthast, Alfred D. French, Thomas Rosenau
Summary: Cellulose model compounds are widely used in cellulose research to study the polymer's properties. By synthesizing methyl 4-O-methyl beta-D-glucopyranoside derivatives with different substitution patterns, the solid-state structures of these compounds were determined using X-ray diffraction. Solid-state C-13 NMR measurements confirmed the influence of packing arrangement on the chemical shifts of the substituted carbons.
Article
Chemistry, Analytical
Di Feng, Daobing Wang, Dongliang Wang, Qiding Zhong, Guohui Li, Luoqi Zhang, Nannan Chen, Xiaoxian Lin, Shu Miao
Summary: This study used stable isotope ratio mass spectrometry to investigate the carbon isotope characteristics of sialic acid in bird's nest products, which can differentiate between natural and synthetic sources. Delta C-13 was proposed as a suitable tool for verifying the authenticity of bird's nest products.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Multidisciplinary
Bastian von Holtum, Maximilian Kubot, Christoph Peschel, Uta Rodehorst, Martin Winter, Sascha Nowak, Simon Wiemers-Meyer
Summary: Despite intensive research in lithium ion and lithium metal batteries, the formation of solid-electrolyte interphase (SEI) in lithium-metal-anode-based battery systems still remains a challenge. This study presents a novel approach using gas, liquid electrolyte, and solid phase accumulation to unravel the SEI compound profile. By leveraging the intrinsic reactivity of lithium metal with the liquid electrolyte, this method offers qualitative and quantitative insights into the wide range of compounds formed in carbonate-based electrolytes through state-of-the-art analytical techniques.
Article
Chemistry, Multidisciplinary
Bastian von Holtum, Maximilian Kubot, Christoph Peschel, Uta Rodehorst, Martin Winter, Sascha Nowak, Simon Wiemers-Meyer
Summary: Despite extensive research in lithium ion and lithium metal batteries, there are still unanswered questions regarding the formation of the solid-electrolyte interphase (SEI) in lithium-metal-anode-based battery systems. This study presents a novel approach that combines the intrinsic behavior of lithium metal with state-of-the-art analytical methods to unravel the compound profile of the SEI. The results reveal the vast variety of compounds formed in carbonate-based electrolytes.
Article
Chemistry, Analytical
Amanda Helms, Edwin E. E. Escobar, Saulius Vainauskas, Christopher H. H. Taron, Jennifer S. S. Brodbelt
Summary: The complete characterization of O-glycopeptides, including identification of peptides, localization of glycosites, and mapping of glycans, has been a challenging task in O-glycoproteomics. This study successfully applied a strategy combining the use of O-glycoprotease IMPa and HCD-triggered UVPD to achieve the complete characterization of O-glycopeptides. This approach allowed the localization of multiple adjacent or proximal O-glycosites and the identification of a previously unknown glycosite on etanercept.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yu-Chieh Huang, Pierre Tremouilhac, An Nguyen, Nicole Jung, Stefan Braese
Summary: ChemSpectra is a web-based software that visualizes and analyzes spectroscopic data, integrating solutions for various types of spectroscopy. It supports open file formats and can be extended to read other formats, making data interoperable and accessible for further analysis and research.
JOURNAL OF CHEMINFORMATICS
(2021)
Article
Chemistry, Multidisciplinary
Anirban Ghosh, Marko Trajkovski, Marie-Paule Teulade-Fichou, Valerie Gabelica, Janez Plavec
Summary: Human telomeric G-quadruplex DNA structures are attractive targets for anticancer drugs. However, the polymorphism of the target complicates drug design. This study reports the discovery of a G-quadruplex ligand that can completely change the fold of the DNA structure, providing valuable insights into the coordination structure of G-quadruplexes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Matthias Brauser, Tim Heymann, Christina Marie Thiele
Summary: In this study, the intermediate responsible for the acyl transfer in a highly enantioselective tetrapeptide organocatalyst was successfully detected and characterized using NMR-spectroscopy and mass spectrometry. The formation of complexes between the tetrapeptide and trans-cyclohexane-1,2-diols was also observed, along with monitoring of the acetylation reaction. These findings provide important insights into the understanding of this organocatalyst's mechanism.
Review
Food Science & Technology
Engy Mahrous, Ruoxin Chen, Chao Zhao, Mohamed A. Farag
Summary: Lipid analysis is crucial for food authentication and quality control, providing consumers with information for informed choices. Recent advancements in lipid analysis and lipidome scope present great opportunities for food science. This review provides a comprehensive overview of tools for extraction, analysis, and interpretation of dietary fats data, discussing different analytical platforms and complementary tools. Applications include quality control, food authentication, tracing fat sources, and studying the effect of heat/storage on lipids. A variety of analytical methods are available, catering to different needs and preferences. This review serves as a navigation tool for food scientists in developing optimal lipid analysis protocols.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Environmental Sciences
Valentin Goeldner, Michael Speitling, Uwe Karst
Summary: The environmental impact of pesticides and other pollutants depends on degradation and accumulation processes. This study investigated the environmental metabolism of a specific herbicide called tritosulfuron, observing a new metabolite through soil degradation studies and using high performance liquid chromatography and mass spectrometry. The structure of the metabolite was elucidated using electrochemistry coupled to mass spectrometry and NMR spectroscopy, highlighting the potential of these techniques in environmental fate studies.
