Journal
CARBOHYDRATE POLYMERS
Volume 98, Issue 1, Pages 988-994Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.07.016
Keywords
Polysaccharides from Enteromorpha prolifera; Degradase; Alteromonas sp A321; Identification; Enzymatic hydrolysis; Response surface methodology
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Funding
- Ocean Public Welfare Scientific Research Project, State Oceanic Administration People's Republic of China [201105028-4]
- Project of Key Laboratory of Active Material and Modern Analysis Technology [MBSMAT-2011-05]
- Direct For Education and Human Resources
- Division Of Human Resource Development [0833178] Funding Source: National Science Foundation
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Polysaccharides from Enteromorpha prolifera (PE) are becoming increasingly popular due to its bioactivity and abundant source. Screening novel microorganisms which could secrete enzymes to degrade PE efficiently for oligosaccharides production is a promising solution to improve its application. In this study, a marine bacterium that can produce enzymes to degrade PE specifically was selected. It was identified as Alteromonas sp. A321, based on the biochemical properties and 16S rDNA gene sequencing. In order to maximize the activity of degradase for polysaccharides from E. prolifera COPE), the effects of medium composition and culture conditions were investigated. The highest DPE production was obtained in the medium consisting of K2HPO4 0.15%, PE 0.9%, NaNO3 0.4%, NaCl 1.0% and MgSO4 0.05%. The degradase activity was enhanced from original 0.391 U/ml to 0.744 U/ml. DPE show high efficiency and substrate specificity to PE with 63.53% of reducing sugar production in the 7 h hydrolysis. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
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