Preparation of resistant starch by hydrolysis of maize starch with pullulanase

In this paper high resistant starch content product was prepared by hydrolyzing of maize starch with pullulanase The optimal hydrolyzing conditions were investigated and the optimum conditions were as follows time 32 h pH 50 temperature 46 C amount of pullulanase 12ASPU/g The product of resistant starch was obtained by pressure-cooking the resulting hydrolysate in an autoclave at 121 C for 1 h cooling at room temperature storing at 4 C overnight autoclaving/cooling for 2 repetition cycle drying an oven (105 C) and finally grounding into fine particles (<150 mu m) The content of resistant starch in the product was 447% (w/w) Results of experiments indicated that this was a promising way of preparation of product of high content of resistant starch (C) 2010 Elsevier Ltd All rights reserved