Journal
CARBOHYDRATE POLYMERS
Volume 81, Issue 3, Pages 560-566Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.03.009
Keywords
Squid ink polysaccharide; Sulfation; Nuclear magnetic resonance migration; Angiogenesis
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Funding
- National High-tech Research and Development Project of China [2006AA09Z444, 2007AA091802]
- National Natural Science Foundation of China [30871944, 30972284]
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This paper is the first to report the preparation, characterization, and potential biological activities of a chemically sulfated polysaccharide isolated from the ink of the squid, Ommastrephes bartrami. The squid ink polysaccharides (SIPS) were firstly sulfated with the pyridine-sulfur-trioxide complex in dimethyl sulfoxide. Structural characterization of sulfated SIP using nuclear magnetic resonance indicated that sulfation mainly occurred at the 4,6-position of GalNAc. The effects of the sulfated SIP (TBA-1) on tumor cell growth, invasion, and migration were examined in vitro, and its effects on angiogenesis were measured in vivo using the chick embryo chorioallantoic membrane (CAM) assay. TBA-1 did not have any obvious effects on the proliferation of HepG2 tumor cells, but induced the dose-dependent suppression of cell invasion and migration in HepG2. Moreover, TBA-1 obviously inhibited angiogenesis in a CAM model. Thus, our results indicate that TBA-1 is a potential candidate compound for the prevention of tumor metastasis. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.
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