Antitumor drug Paclitaxel-loaded pH-sensitive nanoparticles targeting tumor extracellular pH

Research efforts have been devoted to demonstrating that the pH-sensitive characteristics of poly NIPAAm/chitosan nanoparticles can be applied to targeting tumors. A copolymer of (NIPAAm) and chitosan (4:1, m/m) was synthesized, and its drug release characteristics investigated. The results revealed that drug-loaded nanoparticles which encapsulation and loading efficiencies were 85.7% and 9.6%, respectively, exhibited pH-sensitive responses to tumor pH. The cumulative release rate was significantly enhanced below pH 6.8 and decreased rapidly above pH 6.9 at 36.5 +/- 0.5 degrees C. MTT assay and fluorescence microscopic study showed that drug release was drastically promoted in tumor surroundings while exerting less effect in normal conditions. For mice treated with nanoparticles, the decrease in body weight was limited, and significant tumor regression was observed with complete regression in more than 50% of the mice. The life span of tumor-bearing mice was significantly increased when they were treated with nanoparticles. Thereby, the super pH-sensitive poly NIPAAm/chitosan nanoparticles may provide outstanding advantages for anti-cancer drug delivery. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.