Article
Biochemistry & Molecular Biology
Fei Gao, Jianjun Han, Li Jia, Jun He, Yun Wang, Mi Chen, Xiaojun Liu, Xia He
Summary: miR-30c enhances NK cell cytotoxicity to lung cancer cells by decreasing GALNT7 and inactivating the PI3K/AKT pathway, suggesting that regulating miR-30c expression may be a promising approach for enhancing NK cell-based antitumor therapies.
Article
Medicine, Research & Experimental
Zheng He, Meng Tian, Xuan Fu
Summary: miR-30c-5p downregulation in hepatocellular carcinoma is significantly associated with patient survival and tumor size, affecting cell proliferation and migration, with RAB32 identified as a crucial target. These findings highlight the important role of miR-30c-5p in HCC growth and invasion through the miR-30c-5p-RAB32 axis.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Rui Zhang, Qingxi Liu, Sa Zhou, Hongpeng He, Mingfeng Zhao, Wenjian Ma
Summary: In this study, a genetically modified bifunctional CAR-NK cell therapy was developed for the treatment of acute myeloid leukemia (AML). The bifunctional CD33/B16 CAR-NK cells showed superior killing efficiency towards AML cells compared to CAR-NK cells targeting CD33 only. In vivo studies also demonstrated effective clearance of leukemic cells and improved survival. These findings suggest a promising CAR-NK approach for AML treatment and potentially other tumors.
Article
Oncology
Shuyi Han, Yan Qi, Yihui Xu, Min Wang, Jun Wang, Jing Wang, Mingjie Yuan, Yanfei Jia, Xiaoli Ma, Yunshan Wang, Xiangdong Liu
Summary: DLEU2 is significantly up-regulated in GC tissues and cell lines, associated with pathological grading and TNM stage of GC patients. MiR-30a-5p can directly bind to the 3' UTR region of ETS2. DLEU2 binds to miR-30a-5p to facilitate ETS2 expression.
CANCER CELL INTERNATIONAL
(2021)
Article
Immunology
Yan Feng, Yan Li, Ying Zhang, Bo-Hao Zhang, Hui Zhao, Xin Zhao, Fu-Dong Shi, Wei-Na Jin, Xiao-An Zhang
Summary: In this study, researchers investigated the posttranscriptional mechanisms regulating NK cell activation and cytotoxicity after ischemic stroke. They found that miR-1224 plays a significant role in suppressing NK cell activity, while its inhibitor enhances NK cell function and cytotoxicity, particularly in the periphery. This suggests that targeting miR-1224 may be a novel approach to prevent poststroke infection and preserve immune defense in the brain.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Immunology
Alice Mac Donald, Delphine Guipouy, William Lemieux, Mario Harvey, Louis-Jean Bordeleau, David Guay, Hugo Romero, Yuanyi Li, Renaud Dion, Kathie Beland, Elie Haddad
Summary: NK cell therapy has the potential to be a universal off-the-shelf option for cancer treatment. However, the limited efficacy of NK cell-based immunotherapies against solid tumors is a major challenge. In this study, targeting the NKG2A/HLA-E immune checkpoint by using CRISPR-mediated KLRC1 gene editing significantly improved NK cell cytotoxicity against HLA-E+ tumors.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Sagar N. Agnihotri, Giovanni Stefano Ugolini, Matthew Ryan Sullivan, Yichao Yang, Agustin De Ganzo, Ji Won Lim, Tania Konry
Summary: Most common cellular analysis methods employ a top-down approach, which destroys the cell and prevents correlation between genomics and functional assays. This study presents a bottom-up approach using single-cell tools, allowing functional phenotyping through observing cytotoxicity and probing underlying biology.
Article
Biochemistry & Molecular Biology
Kento Takaya, Toru Asou, Kazuo Kishi
Summary: The accumulation of senescent cells in aging tissues is associated with age-related diseases and functional decline. A study found that atypical chemokine receptor 3 (ACKR3) is selectively expressed on the surface of senescent human fibroblasts. This discovery allows for the selective elimination of senescent cells and could contribute to the development of novel senolysis approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Yufan Zhang, Yifan Zhao, Yue Zhai, Jinyi He, Mengke Tang, Yalin Liu, Ye Yao, Peng Xue, Miao He, Qian Li, Yanyi Xu, Weidong Qu, Yubin Zhang
Summary: This study aimed to investigate the impact of cadmium (Cd) on natural killer (NK) cells. It was found that Cd treatment impaired the differentiation of NK progenitors in the bone marrow but activated the JAK3/STAT5 signaling to drive the proliferation of mature NK (mNK) cells. Cd treatment bidirectionally regulated the cytotoxicity of mNK cells to tumor cells through the modulation of Fas expression and granzyme B expression. Similar effects were observed in human mNK cells. This research extends our understanding of the immunotoxicity of Cd.
Article
Cell Biology
Xinchen Yang, Shikun Yang, Jinhua Song, Wenjie Yang, Yang Ji, Feng Zhang, Jianhua Rao
Summary: Research indicates that miR-23b-5p acts as a tumor suppressor in HCC by inducing G0/G1 cell cycle arrest and inhibiting cell proliferation. It targets FOXM1 to regulate cyclin D1 and c-MYC expression, suggesting it may serve as a potential biomarker for HCC diagnosis and prognosis.
CELL DEATH DISCOVERY
(2021)
Article
Oncology
Stefanie Renken, Takahiro Nakajima, Isabelle Magalhaes, Jonas Mattsson, Andreas Lundqvist, Elias S. J. Arner, Rolf Kiessling, Stina Linnea Wickstrom
Summary: Auranofin can significantly reduce intracellular ROS accumulation in human cytotoxic lymphocytes ex vivo, preserving their antitumoral activity and increasing resistance to oxidative stress.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Cell & Tissue Engineering
Qing Zhang, Ting Chen, Yun Zhang, Lingxia Lyu, Bohuan Zhang, Chengchen Huang, Xuhao Zhou, Yutao Wu, Zhoubin Li
Summary: The expression of miR-30c-5p was significantly decreased in atherosclerotic arteries, and it inhibited VSMC differentiation from adventitial Sca-1(+) progenitor cells through targeting OPG, suggesting a new therapeutic target for VSMC-associated diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Rongmu Xia, Guojun Geng, Xiuyi Yu, Zhong Xu, Jing Guo, Hongming Liu, Ning Li, Ziyan Li, Yingli Li, Xiaofang Dai, Qicong Luo, Jie Jiang, Yanjun Mi
Summary: In this study, LINC01140 was found to be highly expressed in human lung cancer tissues and cell lines, promoting proliferation, migration, and invasion of lung cancer cells through interactions with specific microRNAs. Additionally, LINC01140 also contributed to immune escape in lung cancer by upregulating programmed death-ligand 1 (PD-L1) expression.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Multidisciplinary Sciences
Devivasha Bordoloi, Abhijeet J. Kulkarni, Opeyemi S. Adeniji, M. Betina Pampena, Pratik S. Bhojnagarwala, Shushu Zhao, Candice Ionescu, Alfredo Perales-Puchalt, Elizabeth M. Parzych, Xizhou Zhu, Ali R. Ali, Joel Cassel, Rugang Zhang, Michael R. Betts, Mohamed Abdel-Mohsen, David B. Weiner
Summary: Targeting Siglec-7 antigen can enhance the killing effect of natural killer (NK) cells on ovarian cancer cells and improve treatment outcomes. Additionally, the development of a cell engager that activates NK cells and targets ovarian cancer cells has shown promising results in controlling tumor growth and improving survival rates.
Article
Immunology
Wanze Zhang, Bingxin Yu, Qingyu Meng, Luya Pu, Bin Liu, Fan Li
Summary: The novel IFN-like protein Novaferon, generated through gene shuffling, enhances the antitumor activity of NK cells by increasing the secretion of cytokines and upregulating activating receptors. In experiments, NK92-nova cells demonstrated stronger antitumor activity, inhibiting the growth of HepG2 cells without causing systemic toxicity.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Jingchang Ma, Wei Hu, Dongliang Zhang, Jiangang Xie, Chujun Duan, Yitian Liu, Yuling Wang, Xuexue Xu, Kun Cheng, Boquan Jin, Yuan Zhang, Ran Zhuang
Summary: CD226 plays a crucial role in regulating obesity and chronic low-grade inflammation, as its loss can alleviate obesity and systemic inflammation primarily by inhibiting the accumulation and M1 polarization of ATMs.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Shuwen Wu, Yuan Zhang, Jingchang Ma, Yongming Liu, Wenpeng Li, Tingting Wang, Xuexue Xu, Yuling Wang, Kun Cheng, Ran Zhuang
Summary: This study reveals the important role of IL-6 in mucosal immune cells and its interaction with gut microbiota. IL-6 loss can alter the composition of gut microbiota and affect the maintenance of immune cells, triggering mucosal immune response.
Article
Multidisciplinary Sciences
Yang Xie, Yuan Zhang, Yitian Liu, Yuling Wang, Kun Cheng, Ran Zhuang, Ka Bian
Summary: ILC2s have been extensively studied and found to be involved in regulating various pathological processes. However, their exact functions in nasal mucosal immunity and their potential role in related diseases remain unknown. This study established a method to isolate and identify ILC2s from the nasal mucosa and detected CD226 expression on these cells. This protocol will help explore the internal pathological mechanism of immunological disorders in nasal mucosal diseases.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Yang Xie, Yuan Zhang, Tianxiao Zhu, Jingchang Ma, Chujun Duan, Lu Yang, Tingting Wang, Ran Zhuang, Ka Bian, Lianjun Lu
Summary: This study revealed the crucial role of CD226 in allergic inflammatory responses in a murine AR model. Specifically, CD226 may regulate ILC2 function to alleviate allergic and inflammatory responses, rather than affecting CD4(+) T cells.
MEDIATORS OF INFLAMMATION
(2022)
Article
Immunology
Pengcheng Li, Chunmei Zhang, Meng Wang, Xiyue Zhang, Yusi Zhang, Kang Tang, Haifeng Hu, Xiaozhou Jia, Ran Zhuang, Boquan Jin, Ying Ma, Yun Zhang
Summary: This study analyzed the role of neutrophils in HFRS patients. The results showed that plasma MPO and NPA significantly increased in the acute phase and critical/severe groups of HFRS patients and were correlated with inflammatory and renal injury-related clinical parameters. These findings provide new insights into the potential role of neutrophils in the pathogenesis of HFRS.
Article
Biochemistry & Molecular Biology
Yuan Zhang, Xuexue Xu, Jingchang Ma, Yongming Liu, Chujun Duan, Yitian Liu, Chongyang Feng, Wenpeng Li, Yuling Wang, Kun Cheng, Ran Zhuang
Summary: CD226 plays a crucial role in the development of atherosclerosis by promoting foam cell formation. CD226 deficiency reduces plaque accumulation and inhibits atherosclerosis progression.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Virology
Huiyuan Zhang, Yazhen Wang, Ying Ma, Kang Tang, Chunmei Zhang, Meng Wang, Xiyue Zhang, Manling Xue, Xiaozhou Jia, Haifeng Hu, Na Li, Ran Zhuang, Boquan Jin, Lihua Chen, Yun Zhang, Yusi Zhang
Summary: This study characterized double positive T (DP T) cells in HFRS patients using flow cytometry data combined with scRNA-seq data. Results showed an upregulation of DP T cells in the peripheral blood during HTNV infection, which was correlated with disease stage. The scRNA-seq data clustered and unraveled the gene expression profile of DP T cells.
Article
Immunology
Yusi Zhang, Meng Wang, Xiyue Zhang, Kang Tang, Chunmei Zhang, Xiaozhou Jia, Haifeng Hu, He Liu, Na Li, Ran Zhuang, Boquan Jin, Ying Ma, Yun Zhang
Summary: This study characterized CD8(+)MAIT cells from HFRS patients using scRNA-seq and flow cytometry. The activation and deficiency of CD8(+)MAIT cells in peripheral blood during HTNV infection were observed, which correlated with disease severity. CD8(+)MAIT cells showed anti-viral properties by producing granzyme B and IFN-gamma, and limiting HTNV replication. The activation of MAIT cells was mediated by IL-18, sourced from HTNV-infected monocytes.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2023)
Article
Immunology
Jingchang Ma, Yitian Liu, Chujun Duan, Shuwen Wu, Yang Xie, Lu Yang, Xuemei Li, Yuling Wang, Yuan Zhang, Ran Zhuang
Summary: The costimulation molecule CD226 plays a significant role in T cell differentiation, activation, and immune functional regulation. Its deficiency impairs immune response capacity and affects thymocyte development, particularly the maturation of CD8+ T cells.
Article
Infectious Diseases
Kang Tang, Yusi Zhang, Chunmei Zhang, Haifeng Hu, Ran Zhuang, Boquan Jin, Yun Zhang, Ying Ma
Summary: This study found that plasma OPG levels were elevated in patients with HFRS and correlated positively with disease severity and negatively with platelet counts. Endothelial cells released abundant OPG in response to TNF-α stimuli and HTNV infection, which correlated positively with plasma TNF-α and c-reactive protein. Increased plasma OPG levels induced by HTNV may be an important factor for the severity of HFRS and could contribute to endothelial dysfunction and hemorrhagic disorder.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Xiyue Zhang, Yusi Zhang, He Liu, Kang Tang, Chunmei Zhang, Meng Wang, Manling Xue, Xiaozhou Jia, Haifeng Hu, Na Li, Ran Zhuang, Boquan Jin, Fanglin Zhang, Yun Zhang, Ying Ma
Summary: This study aimed to investigate the effect of bystander-activated CD8+ T cell responses in HTNV infection. An in vitro infection model was established to mimic endothelium injury in HFRS patients. The results showed that HTNV infection could activate EBV or CMV-specific CD8+ T cells and induce high levels of IL-15 production in HTNV-infected HUVECs, which were correlated with disease severity and NKG2D expression on bystander-activated CD8+ T cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Yuan Zhang, Chujun Duan, Shuwen Wu, Jingchang Ma, Yongming Liu, Wenpeng Li, Tingting Wang, Lu Yang, Kun Cheng, Ran Zhuang
Summary: IL-6 deficiency alleviates acute CWIR-induced intestinal damage and apoptosis, and improves stress defense. This finding sheds new light on treating CWIR-induced intestinal disorders by inhibiting IL-6 signaling.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Surgery
Dongliang Zhang, Yitian Liu, Jingchang Ma, Zhigang Xu, Chujun Duan, Yuling Wang, Xuemei Li, Juntao Han, Ran Zhuang
Summary: This study established a transplantation rejection model to evaluate the regulation of the poliovirus receptor signaling pathway using CD226 knockout or TIGIT-Fc recombinant protein treatment. The results showed that TIGIT-Fc treatment and CD226 knockout prolonged graft survival time, increased regulatory T cell proportion, and induced M2-type macrophage polarization. Donor-reactive recipient T cells became hyporesponsive but still responded normally to a third-party antigen challenge.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Immunology
Tingting Wang, Wei Qiao, Yang Xie, Jingchang Ma, Wei Hu, Lu Yang, Xuemei Li, Chujun Duan, Shuwen Wu, Yuling Wang, Kun Cheng, Yuan Zhang, Ran Zhuang
Summary: In this study, CD226 deficiency was found to result in more severe skin pathologies and bowel abnormalities in an AD-like mouse model. CD226 expression was elevated in intestinal mucosal tissues following DNCB administration. CD226 deficiency led to an overactive intestinal immune response, characterized by increased inflammation and cytokine levels, Paneth cell numbers, and AMP expression.
Article
Microbiology
Shanshou Liu, Dongqing Wen, Chongyang Feng, Chaoping Yu, Zhao Gu, Liping Wang, Zhixiang Zhang, Wenpeng Li, Shuwen Wu, Yitian Liu, Chujun Duan, Ran Zhuang, Lihao Xue
Summary: Heat acclimation training (HAT) can prevent heat-related diseases. This study found that HAT has beneficial effects on organ function, immunity, and gut microbes, with a stronger promotion of probiotics in a high temperature and humidity environment.
FRONTIERS IN MICROBIOLOGY
(2023)
