Journal
ANALYTICAL SCIENCES
Volume 31, Issue 9, Pages 867-873Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.31.867
Keywords
Microfluidics; fluorescence in situ hybridization; DNA; circulating tumor cells; pathology
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Funding
- NEDO Industrial Technology Research Assistance Project
- JSPS, KAKENHI [15K15199]
- Shimadzu Science Foundation
- Grants-in-Aid for Scientific Research [15K15199] Funding Source: KAKEN
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Microfluidic devices enable the miniaturization, integration, automation, and parallelization of chemical and biochemical processes. This new technology also provides opportunity for expansion in the field of cellular pathology. Fluorescence in situ hybridization (FISH) is a well-known gene-based method to image genetic abnormalities. Development of a FISH microfluidic platform has offered the possibility of automation with significant time and cost reductions, which overcomes many drawbacks of the current protocols. Microfluidic devices are also powerful tools for single-cell analysis. Capturing the circulating tumor cells (CTCs) from blood samples is one of the most promising approaches to enable the early diagnosis of cancer. The microfluidic devices are also useful to isolate rare CTCs at high efficiency and purity. In this review, I outline recent FISH and CTC analyses using microfluidic devices, and describe their applications for the cellular diagnosis of cancers.
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