Journal
ANALYTICAL SCIENCES
Volume 31, Issue 11, Pages 1171-1175Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.31.1171
Keywords
Microchip electrophoresis; vacuum-drying method; polymer coating; electroosmotic flow; bioanalysis
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Funding
- Japan Society for the Promotion of Science (JSPS) [24550090, 15K05527]
- SENTAN, JST
- Grants-in-Aid for Scientific Research [26410159, 15K13756, 24550090, 15K05527] Funding Source: KAKEN
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A simple and rapid vacuum-drying modification method was applied to several neutral and charged polymers to obtain coating layers for controlling electroosmotic flow (EOF) and suppressing sample adsorption on poly(dimethyl siloxane) (PDMS)-glass hybrid microchips. In the vacuum-dried poly(vinylpyrrolidone) coating, the electroosmotic mobility (mu(eo)) was suppressed from +2.1 to +0.88 x 10(-4) cm(2)/V.s, and the relative standard deviation (RSD) of mu(eo) was improved from 10.2 to 2.5% relative to the bare microchannel. Among several neutral polymers, poly(vinylalcohol) (PVA) and poly(dimethylacrylamide) coatings gave more suppressed and repeatable EOF with RSDs of less than 2.3%. The vacuum-drying method was also applicable to polyanions and polycations to provide accelerated and inversed EOF, respectively, with acceptable RSDs of less than 4.9%. In the microchip electrophoresis (MCE) analysis of bovine serum albumin (BSA) in the vacuum-dried and thermally-treated PVA coating channel, an almost symmetric peak of BSA was obtained, while in the native microchannel a significantly skewed peak was observed. The results demonstrated that the vacuum-dried polymer coatings were effective to control the EOF, and reduced the surface adsorption of proteins in MCE.
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