Article
Optics
Jeremy Xuan Yu Chew, Zi Heng Lim, Y. Qi, Guangcan Zhou, Guangya Zhou
Summary: Infrared devices are increasingly used in various applications. This paper introduces a novel dispersive spectrometer that has high-throughput and decoupled spectral resolution. The proposed spectrometer utilizes a two-stage Hadamard transform encoding process to increase the signal-to-noise ratio. With a single-pixel detector, the system can be easily implemented in other desired wavelengths. Additionally, a method is developed to remove the need for uniform illumination and improve the ease of device design for in situ measurement.
Article
Chemistry, Analytical
Xiaolong Sheng, Jiaqi Xu, Yi Sun, Jinyun Zhao, Yong Cao, Liyuan Jiang, Tianding Wu, Hongbin Lu, Chunyue Duan, Jianzhong Hu
Summary: Macrophages play a crucial role in phagocytizing and clearing myelin debris in spinal cord injury, traumatic brain injury, and ischemic stroke. The biochemical phenotypes of macrophages after phagocytosis show significant heterogeneity. In this study, Synchrotron radiation-based Fourier transform infrared microspectroscopy was used to study the biochemical changes in macrophages after myelin debris phagocytosis, revealing dynamic and significant changes in proteins and lipids. This technique provides an evaluation strategy for studying cell functions related to cellular substance distribution and metabolism.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Tobias P. Woerner, Konstantin Aizikov, Joost Snijder, Kyle L. Fort, Alexander A. Makarov, Albert J. R. Heck
Summary: By investigating the behavior of macromolecular single ions within the Orbitrap analyzer, this study aimed to enhance the performance of charge-detection mass spectrometry. The results showed that ions with a mass beyond one megadalton can be successfully trapped for seconds, enabling precise mass analysis with a resolution of over 100,000. It was also found that the frequency drift of single ions due to desolvation and charge stripping can be corrected, resulting in an improved ion sampling efficiency and mass precision and resolution.
Article
Spectroscopy
Xiaojie Zhou, Jiajia Zhong, Wenjie Yu, Yuzhao Tang
Summary: In this study, the biomolecular changes in normal human hepatocytes induced by doxorubicin treatment were investigated using SR-FTIR microspectroscopy, CCK-8 assay, and flow cytometry. The results showed that the order of lipid acyl chains and protein alpha-helix structures in doxorubicin-treated hepatocytes was significantly altered, while nucleic acids remained relatively unchanged. These findings provide important insights into the mechanism of doxorubicin-induced hepatotoxicity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Environmental Sciences
Marta Potrykus, Vladyslav Redko, Katarzyna Glowacka, Agnieszka Piotrowicz-Cieslak, Pawel Szarlej, Helena Janik, Lidia Wolska
Summary: Plastic waste in landfills can be inhabited by microorganisms from the ambient environment, potentially leading to accelerated degradation involving the formation of biofilm. Further research is needed to investigate the possible risk factors of plastic biodegradation in waste landfills.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Multidisciplinary
Ines Delfino, Valerio Ricciardi, Maria Lepore
Summary: Fourier transform infrared microspectroscopy using a synchrotron radiation source has great potential in studying the ionizing radiation effects on human cells. It has been successfully employed in shedding light on cell processes exposed to hadron therapy, and can contribute to the improvement of cancer treatment strategies.
APPLIED SCIENCES-BASEL
(2022)
Article
Optics
Hongnan Xu, Yue Qin, Gaolei Hu, Hon Ki Tsang
Summary: This paper presents a spectrometer design that surpasses the resolution-bandwidth limit, by tailoring the dispersion of mode splitting in a photonic molecule to identify spectral information at different free-spectral ranges (FSRs). Experimental results demonstrate that this approach can resolve arbitrary spectra with discrete, continuous, or hybrid features.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Agriculture, Dairy & Animal Science
Sasikan Katemala, Amonrat Molee, Kanjana Thumanu, Jirawat Yongsawatdigul
Summary: Chicken breed plays a significant role in meat quality, and the quality attributes of different chicken breeds were analyzed using spectroscopy and physicochemical analysis. The results showed distinct differences in meat quality among commercial broilers, Thai native chickens, and crossbred Korat chickens.
Article
Spectroscopy
Fatih Celal Alcicek, Aneta Blat, Wiktoria Rutkowska, Katarzyna Bulat, Ewa Szczesny-Malysiak, Magdalena Franczyk-Zarow, Renata Kostogrys, Jakub Dybas, Katarzyna M. Marzec
Summary: In this study, the secondary structure alterations of intact red blood cell cytosol were characterized using Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR). The study focused on the sex-related alterations in ApoE/LDLR(-/-) mice and age-matched control animals. The results showed that older female mice had a higher resistance to secondary structure changes in hemoglobin with the progression of atherosclerosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Hancheng Lin, Zhimin Wang, Yiwen Luo, Zijie Lin, Guanghui Hong, Kaifei Deng, Ping Huang, Yiwen Shen
Summary: Diabetic cardiomyopathy (DbCM) is a serious complication of diabetes that affects approximately 12% of diabetic individuals. This study utilized Fourier transform infrared (FTIR) spectroscopy and machine learning algorithms to analyze myocardial tissues of mouse models with type 2 diabetes mellitus (T2DM). The results revealed significant changes in lipid, carbohydrate, and protein composition in the diabetic mice myocardial tissues compared to healthy mice. The spectral lipidomic profiles also demonstrated a time-dependent progression during the development of DbCM. A random forest classifier was developed with a 97.1% accuracy for diagnosing DbCM. This study demonstrates the potential of FTIR microspectroscopy as a novel method for early detection of biochemical changes in the myocardia of mice with T2DM.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Magdalena Szczerbowska-Boruchowska, Patryk Stec, Mateusz Czyzycki, Zbigniew Szczerbowski, Rolf Simon, Tilo Baumbach, Agata Ziomber-Lisiak
Summary: The study proposes a method for using total absorbance under FTIR spectra as a mass surface correction procedure for XRF microimaging of tissues, and compares it with other correction methods. The results show that this method can effectively eliminate the surface mass effect and obtain surface mass-independent elemental quantities.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Multidisciplinary Sciences
Jing Wang, Steven Randolph, Qian Wu, Aurelien Botman, Jenna Schardt, Cedric Bouchet-Marquis, Xiaolin Nan, Chad Rue, Marcus Straw
Summary: A CLEM workflow based on a novel FIB/SEM technology using an OSSM process was proposed, which achieved high resolution and imaging efficiency while improving throughput. By combining with iPALM technique, successful 3D imaging of mitochondria was carried out, demonstrating the advantages of OSSM-SEM in CLEM applications.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Jiawei Wang, Zhen Zhu, Ruyao Li, Ruihua Yu, Wanli Yang, Yaosheng Wang, Chao Yang, Feng Jiang
Summary: The study demonstrated that microglia may enhance the proliferation and migration ability of tumor cells by altering the structures and contents of biomolecules within the tumor cells. This discovery provides new insights into the mechanisms of brain metastasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Lewis M. Dowling, Paul Roach, Eirik A. Magnussen, Achim Kohler, Srinivas Pillai, Daniel G. van Pittius, Ibraheem Yousef, Josep Sule-Suso
Summary: Liquid biopsies have the potential to improve personalized cancer management, but current methods for identifying circulating tumor cells are not reliable enough. Fourier Transform Infrared (FTIR) microspectroscopy could be a new technique for identifying these cells, regardless of tumor type.
Article
Agriculture, Dairy & Animal Science
K. R. Callero, E. M. Teplitz, D. M. Barbano, C. R. Seely, J. A. Seminara, I. R. Frost, H. A. McCray, R. M. Martinez, A. M. Reid, J. A. A. McArt
Summary: Cows undergo physiological stress during early lactation and monitoring milk constituents using FTIR spectroscopy can help understand successful transition. This study aimed to describe the daily patterns of milk constituents in early lactation for cows of different parity groups. Significant differences were found between parity groups in milk constituents, and it is crucial to consider parity when measuring milk constituents using FTIR.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Neurosciences
Anna Konopka, Donna R. Whelan, Md Shafi Jamali, Emma Perri, Hamideh Shahheydari, Reka P. Toth, Sonam Parakh, Tina Robinson, Alison Cheong, Prachi Mehta, Marta Vidal, Audrey M. G. Ragagnin, Ivan Khizhnyak, Cyril J. Jagaraj, Jasmin Galper, Natalie Grima, Anand Deva, Sina Shadfar, Garth A. Nicholson, Shu Yang, Suzanne M. Cutts, Zuzana Horejsi, Toby D. M. Bell, Adam K. Walker, Ian P. Blair, Julie D. Atkin
MOLECULAR NEURODEGENERATION
(2020)
Article
Multidisciplinary Sciences
L. Van Schaik, C. Kettle, R. Green, W. Sievers, M. W. Hale, H. R. Irving, D. R. Whelan, J. A. Rathner
Summary: The study found that low doses of caffeine can activate brown adipose tissue thermogenesis through the central nervous system, increasing heat production without adverse cardiovascular effects.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Donna R. Whelan, Wei Ting C. Lee, Frances Marks, Yu Tina Kong, Yandong Yin, Eli Rothenberg
Review
Chemistry, Analytical
Esther L. Miriklis, Ashley M. Rozario, Eli Rothenberg, Toby D. M. Bell, Donna R. Whelan
Summary: Super-resolution microscopy techniques are well-suited for probing the nanoscale landscape of genomic function and dysfunction, offering high spatial resolution and single molecule localization advantages. These advances have provided unprecedented insights into DNA structure and function and enabled characterization of subpopulations within a single cell. Ongoing development and application of these interdisciplinary techniques will continue to expand the toolbox available for research into the nanoscale genomic landscape.
METHODS AND APPLICATIONS IN FLUORESCENCE
(2021)
Article
Biology
Ashley M. Rozario, Sam Duwe, Cade Elliott, Riley B. Hargreaves, Gregory W. Moseley, Peter Dedecker, Donna R. Whelan, Toby D. M. Bell
Summary: The study reveals that low concentrations of colcemid cause abnormal microtubule curvature, with a trend of increased curvature associated to higher doses, and curvatures greater than 2 rad/μm being linked to microtubule breakage. Higher doses (>100 nM) of colcemid result in microtubule fragmentation, while lower doses (<20 nM after 5 h) lead to subtle but significant microtubule architecture remodeling and suppression of microtubule dynamics.
Article
Chemistry, Analytical
Riley B. Hargreaves, Ashley M. Rozario, Thomas M. McCoy, Shane P. Meaney, Alison M. Funston, Rico F. Tabor, Donna R. Whelan, Toby D. M. Bell
Summary: Correlative imaging methods provide more information for investigations of cellular ultra-structure, complementing each other's strengths and compensating for deficiencies. This study optimized the sample preparation for correlative imaging of the cellular cytoskeleton using super-resolution and atomic force microscopies.
METHODS AND APPLICATIONS IN FLUORESCENCE
(2022)
Article
Biochemistry & Molecular Biology
RileyB. Hargreaves, Sam Duwe, Ashley M. Rozario, Alison M. Funston, Rico F. Tabor, Peter Dedecker, Donna R. Whelan, Toby D. M. Bell
Summary: Standard optical imaging lacks the resolving power to visualize organelles and proteins in cells, but super-resolution techniques have overcome this limitation. This study combines live-cell Super-Resolution Optical Fluctuation Imaging (SOFI) with Single Molecule Localization Microscopy (SMLM) and Atomic Force Microscopy (AFM) to observe cellular ultrastructure in live cells. The comparison between live-cell SOFI and fixed-cell imaging shows minimal changes in the imaged target structures.
ACS BIO & MED CHEM AU
(2023)
Article
Biochemistry & Molecular Biology
Jiaul Islam, Paul Conroy, Christian Fercher, Mijin Kim, Zvi Yaari, Martina Jones, Toby D. M. Bell, Tom Caradoc-Davies, Ruby Law, James Whisstock, Daniel Heller, Stephen Mahler, Simon Corrie
Summary: Reagentless immunosensors are being developed for practical and sensitive detection of biomarkers in biological samples without the need for multistep assays. This study used a structure-guided approach to identify a mutation site in an antibody fragment for polarity-dependent fluorophores, allowing for detectable wavelength shifts upon binding to the protein target. The results showed significant emission wavelength shifts and demonstrated the potential of incorporating different dyes into the same mutation site. These reagents have applications in research, medical laboratories, and point-of-care platforms to create simpler and more efficient biosensors.
ACS CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Gangcheng Yuan, Heather F. Higginbotham, Jiho Han, Anchal Yadav, Nicholas Kirkwood, Paul Mulvaney, Toby D. M. Bell, Jared H. Cole, Alison M. Funston
Summary: This study investigates the photoluminescence anisotropy of CdSe semiconductor nanocrystals experimentally and theoretically. By visualizing emission dipole orientation using defocused wide-field microscopy, it is found that different shaped nanocrystals exhibit different optical properties. The differences in defocused emission patterns of rods and long nanoplatelets are explained by considering valence band structures calculated using multiband effective mass theory and the dielectric effect.
Article
Polymer Science
Axel-Laurenz Buckinx, Ashley M. Rozario, Toby D. M. Bell, Jasper J. Michels, Tanja Junkers
Summary: This study presents a novel method for synthesizing semi-conducting polymer nanoparticles (SPN) and demonstrates their potential as versatile theragnostic platforms for drug delivery and near infrared (NIR) imaging.
Article
Chemistry, Physical
Heather F. Higginbotham, Subashani Maniam, Tina Hsia, Lyle Isaacs, Steven J. Langford, Toby D. M. Bell
Summary: NDIs are shown to spontaneously arrange with CB[8] in an aqueous environment through purely non-covalent interactions, resulting in a highly fluorescent 2 : 2 supramolecular complex. The increased fluorescence is attributed to the formation of NDI-NDI excimers within the supramolecular complex.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Polymer Science
Yanallah Alqarni, Fumi Ishizuka, Toby D. M. Bell, Rico F. Tabor, Per B. Zetterlund, Kei Saito
Article
Chemistry, Multidisciplinary
Elley E. Rudebeck, Rosalind P. Cox, Toby D. M. Bell, Rameshwor Acharya, Zikai Feng, Nuri Gueven, Trent D. Ashton, Frederick M. Pfeffer
CHEMICAL COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Rosalind P. Cox, Saman Sandanayake, Steven J. Langford, Toby D. M. Bell
AUSTRALIAN JOURNAL OF CHEMISTRY
(2020)
Correction
Chemistry, Multidisciplinary
Ashley M. Rozario, Fabian Zwettler, Sam Duwe, Riley B. Hargreaves, Aaron Brice, Peter Dedecker, Markus Sauer, Gregory W. Moseley, Donna R. Whelan, Toby D. M. Bell
AUSTRALIAN JOURNAL OF CHEMISTRY
(2020)