Article
Cardiac & Cardiovascular Systems
Christine Veith, Ipek Varturk-Ozcan, Magdalena Wujak, Stefan Hadzic, Cheng-Yu Wu, Fenja Knoepp, Simone Kraut, Aleksandar Petrovic, Marija Gredic, Oleg Pak, Monika Brosien, Marie Heimbrodt, Jochen Wilhelm, Friederike C. Weisel, Kathrin Malkmus, Katharina Schaefer, Henning Gall, Khodr Tello, Djuro Kosanovic, Akylbek Sydykov, Akpay Sarybaev, Andreas Guenther, Ralf P. Brandes, Werner Seeger, Friedrich Grimminger, Hossein A. Ghofrani, Ralph T. Schermuly, Grazyna Kwapiszewska, Natascha Sommer, Norbert Weissmann
Summary: This study provides evidence for the involvement of SPARC in the pathogenesis of human PH and chronic hypoxia-induced PH in mice, most likely by affecting vascular cell function.
Article
Cell & Tissue Engineering
Ning Zhou, Lei Wang, Ping Fu, Zihao Cui, Yuhang Ge, Feiyu Jiang, Jing Liu, Chao Ren, Zuo Luan, Hongbin Fan, Ruiqin Yao
Summary: EPCs enhance oligovascular remodeling and decrease OPC apoptosis, while EC-pEPCs are more effective in promoting oligovascular remodeling and myelination through the CXCL12-CXCR4 axis in the neonatal rat WMI model. EPCs and CXCL12 protein levels significantly increase in WMI rats, and EC-pEPCs show notable improvements in vascular density, myelination, and cognitive outcomes compared to EPCs.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Pharmacology & Pharmacy
Yongpan Huang, Chong Song, Jianbin He, Min Li
Summary: Endothelial cells, as important metabolic and endocrine cells, play a crucial role in regulating vascular function. Various cardiovascular and cerebrovascular diseases are linked to endothelial dysfunction. The mechanisms of endothelial injury involve inflammation and oxidative stress, and endothelial progenitor cells are believed to contribute to the repair of damaged endothelium.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Oncology
Ghazaleh Hashemi, James Dight, Kiarash Khosrotehrani, Laura Sormani
Summary: Melanoma is an aggressive and potentially lethal form of skin cancer. The role of blood vessel formation, particularly endothelial progenitor cells (EPCs), in melanoma is of significance. Understanding the role of EPCs is important for understanding the abnormal vessel structures in melanoma. The effect of anti-angiogenic drugs on melanoma treatment needs further research.
Review
Biochemistry & Molecular Biology
Amankeldi A. Salybekov, Shuzo Kobayashi, Takayuki Asahara
Summary: This article discusses the challenges and issues surrounding the use of endothelial progenitor cells (EPCs) as candidate cell sources for revascularization strategies. It also highlights the origins, sources, isolation, and identification techniques of EPCs, as well as the diversity of human and murine EPC markers. Furthermore, it summarizes the current and future prospects of the crosstalk between EPCs and the biology of hematopoietic cells, as well as culture techniques in the context of regeneration-associated cells (RACs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Laura Casarrubios, Alberto Polo-Montalvo, Maria Concepcion Serrano, Maria Jose Feito, Maria Vallet-Regi, Daniel Arcos, Maria Teresa Portoles
Summary: This study demonstrated the potential of nanoparticles to enhance VEGFR2 expression and angiogenesis in endothelial progenitor cells (EPCs) by intracellular incorporation through endocytosis mechanisms. The nanospheres showed angiogenic effects on EPCs even in the presence of phagocytic cells, confirming their role in promoting vascularization and tissue regeneration.
Article
Engineering, Biomedical
Zhiming Shen, Fei Sun, Yibo Shan, Yi Lu, Cong Wu, Boyou Zhang, Qiang Wu, Lei Yuan, Jianwei Zhu, Qi Wang, Yilun Wang, Wenxuan Chen, Yaojing Zhang, Wenlong Yang, Yiwei Fan, Hongcan Shi
Summary: A novel cell-free tissue-engineered tracheal scaffold was developed to promote vascularization using endothelial progenitor cell exosomes. The results demonstrated the excellent angiogenic potential of the scaffold and its capacity to repair tracheal defects.
MATERIALS TODAY BIO
(2023)
Article
Oncology
Dimitri G. Trembath, Eric S. Davis, Shanti Rao, Evan Bradler, Angelica F. Saada, Bentley R. Midkiff, Anna C. Snavely, Matthew G. Ewend, Frances A. Collichio, Carrie B. Lee, Georgia-Sofia Karachaliou, Fatih Ayvali, David W. Ollila, Michal T. Krauze, John M. Kirkwood, Benjamin G. Vincent, Nana Nikolaishvilli-Feinberg, Stergios J. Moschos
Summary: The presence of high tumor-infiltrating lymphocytes (TILs) in patients undergoing craniotomy for melanoma brain metastases (MBM) before 2011 may have prognostic significance. Intratumoral hemorrhage is associated with tumor volume, high expression of basic fibroblast growth factor (bFGF), and high density of CD31+alpha SMA- blood vessels.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Yi-Nan Lee, Hsueh-Hsiao Wang, Cheng-Huang Su, Hsin- Lee, Yen-Hung Chou, Chin-Ling Hsieh, Wen-Ting Liu, Kuo-Tung Shu, Kai-Ting Chang, Hung- Yeh, Yih-Jer Wu
Summary: The study established an accelerated senescence model by treating endothelial progenitor cells (EPCs) with the iron chelator deferoxamine (DFO). Results showed that DFO treatment induced senescent phenotypes in EPCs and could validate its effects in vivo.
Article
Biochemistry & Molecular Biology
Sawssen Ben Fraj, Sina Naserian, Bileyle Lorenzini, Sylvie Goulinet, Philippe Mauduit, Georges Uzan, Houda Haouas
Summary: Circulating endothelial progenitor cells (EPCs) play a pivotal role in angiogenesis-related diseases. However, their clinical use is limited due to storage conditions and immune rejection. EPC-derived extracellular vesicles (EPC-EVs) may be an alternative, as they enhance EPC regenerative functions without altering their endothelial identity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Michael Warkala, Dongying Chen, AnnJosette Ramirez, Ali Jubran, Michael Schonning, Xia Wang, Huaning Zhao, Sophie Astrof
Summary: The development of the fourth pharyngeal arch arteries involves a multistep process with contributions from SHF-derived ECs, remodeling of endothelial plexus into PAAs, and remodeling of PAAs into the aortic arch artery and its major branches. Cell-ECM interactions regulated by integrin alpha 5 beta 1 and Fn1 are essential at each developmental stage.
CIRCULATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jieun Lee, Hal Sternberg, Paola A. Bignone, James Murai, Nafees N. Malik, Michael D. West, Dana Larocca
Summary: This study presents a panel of clonal embryonic endothelial progenitor cell lines derived from human embryonic stem cells. These cell lines exhibit characteristics of endothelial cells and show strong transcriptomic similarity to adult endothelial primary cell lines. The cells have demonstrated scalability and stable angiogenic properties, making them a potential source for therapeutic cells in cardiovascular disease treatment.
Article
Endocrinology & Metabolism
Guy S. Taylor, Andy Shaw, Jadine H. Scragg, Kieran Smith, Matthew D. Campbell, Timothy J. McDonald, James A. Shaw, Mark D. Ross, Daniel J. West
Summary: This study found that residual beta-cell function in individuals with type 1 diabetes is associated with increased production of hematopoietic and endothelial progenitor cells in response to exercise. Despite an increase in these cells after exercise in diabetes patients, those without residual beta-cell function still had lower cell counts compared to those with residual beta-cell function at rest.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Engineering, Biomedical
Shirin Changizi, Isabel G. Marquette, Jennifer Vansant, Osamah Alghazwat, Adnan Elgattar, Yi Liao, Chris A. Bashur
Summary: This study developed ultrasound-sensitive microbubbles loaded with carbon monoxide, which can promote endothelial cell proliferation and function. The experiment showed that these microbubbles can improve endothelial cell expression and proliferation, and can be efficiently ruptured by an imaging ultrasound probe.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Physics, Multidisciplinary
Qi Zhong, Chen-Xu Wu, Rui Ma
Summary: The cell wall is an important model system in biology, preventing over-expansion of cells when exposed to water. This paper examines the deformation of a membrane patch by osmotic pressure through a nanopore on the cell wall. The study reveals a critical pore size or pressure beyond which the membrane cannot withstand, leading to inflation through the pore. The critical pore size is linearly related to membrane tension and quadratically related to spontaneous curvature. The critical pressure is inversely proportional to pore radius. The results suggest that membrane bending rigidity in walled cells should be larger than that of mammalian cells to prevent membrane inflation through cell wall pores.
Article
Nutrition & Dietetics
Perrine Vermonden, Matthias Vancoppenolle, Emeline Dierge, Eric Mignolet, Geraldine Cuvelier, Bernard Knoops, Melissa Page, Cathy Debier, Olivier Feron, Yvan Larondelle
Summary: Plant-derived conjugated linolenic acids (CLnA), especially punicic acid (PunA) found in pomegranate seed oil, have been shown to have anti-cancer effects. PunA is highly cytotoxic to colorectal and hypopharyngeal carcinoma cells, triggering ferroptosis. Combining PunA with the known anticancer fatty acid DHA synergistically increases its cytotoxicity. This highlights the potential of using PunA as a ferroptosis-sensitizing phytochemical for cancer prevention and treatment.
Article
Oncology
Chloe Buyse, Nicolas Joudiou, Cyril Corbet, Olivier Feron, Lionel Mignion, Julien Flament, Bernard Gallez
Summary: The dysregulated metabolism and acidosis in tumors play a crucial role in cancer progression and drug resistance. Inhibiting mitochondrial pyruvate carrier (MPC) can lead to tumor acidification, but the response to this treatment varies within the tumor, as shown by high-resolution CEST-MRI parametric images.
Article
Chemistry, Analytical
Kaatje Van Vlierberghe, Maxime Gavage, Marc Dieu, Patsy Renard, Thierry Arnould, Nathalie Gillard, Katleen Coudijzer, Marc de Loose, Kris Gevaert, Christof Van Poucke
Summary: This study aims to identify stable peptide markers for milk for the ultrahigh performance liquid chromatography-tandem mass spectrometry based detection of food allergens in different food products. By establishing tryptic peptide profiles from milk-incurred food materials undergoing various processing techniques, eight peptide biomarkers were selected for application in ultrahigh performance liquid chromatography-tandem mass spectrometry based milk detection methods. These robust markers can be used for the development of a robust detection method for milk in different food matrixes.
JOURNAL OF AOAC INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Virgile Neyman, Frederic Francis, Andre Matagne, Marc Dieu, Catherine Michaux, Eric A. Perpete
Summary: Insect trehalases, essential for trehalose metabolism and stress resistance, were studied with a focus on the extraction and purification of Acyrthosiphon pisum soluble trehalase (ApTreh-1) and its biochemical and structural characterization. The enzyme showed optimal activity at 45 degrees C and pH 5.0, with specific kinetic properties.
Article
Archaeology
Jorsua Herrera Bethencourt, Ismael Rodriguez Palomo, Simon Hickinbotham, Bharath Nair, Silvia Soncin, Marc Dieu, Matthew Collins, Olivier Deparis
Summary: The dataset provides a comprehensive biocodicological analysis of medieval library books and charters using Zooarchaeological Mass Spectrometry (ZooMS). It includes peptide mass fingerprinting and sequencing analysis of 68 codices and 59 charters from the Cistercian abbey of Orval in Belgium. The mass spectrometric data is valuable for understanding parchment production and use in medieval Europe.
JOURNAL OF OPEN ARCHAEOLOGY DATA
(2022)
Article
Chemistry, Analytical
Maxime Gavage, Kaatje Van Vlierberghe, Marc Dieu, Patsy Renard, Thierry Arnould, Marc De Loose, Kris Gevaert, Nathalie Gillard, Christof Van Poucke
Summary: This study has successfully developed a robust method for detecting and quantifying multiple allergens in food, ensuring the safety of allergic consumers. The combination of specific incurred test materials, standard addition strategy, and stable isotope-labeled concatemer has provided the method with sufficient sensitivity and reliability.
JOURNAL OF AOAC INTERNATIONAL
(2022)
Article
Immunology
Lindsay G. A. McKay, Jordan Thomas, Wejdan Albalawi, Antoine Fattaccioli, Marc Dieu, Alessandra Ruggiero, Jane A. McKeating, Jonathan K. Ball, Alexander W. Tarr, Patricia Renard, Georgios Pollakis, William A. Paxton
Summary: HCV glycoprotein inhibits HIV-1 LTR activation by suppressing NF-kappa B activity, potentially affecting the clearance of infection. This process may be influenced by host and viral genetic factors, as well as HCV encoded immunomodulatory proteins. Additionally, HCV glycoprotein can activate endoplasmic reticulum stress pathway and upregulate related gene expression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Sophie Ayama-Canden, Rodolfo Tondo, Liliana Pineros, Noelle Ninane, Catherine Demazy, Marc Dieu, Antoine Fattaccioli, Tijani Tabarrant, Stephane Lucas, Davide Bonifazi, Carine Michiels
Summary: In the context of breast cancer metastasis study, the study investigated the regulation mechanism of cell migration and found that the IGDQ fibronectin motif activates integrin and influences cell migration. Integrins α5 and αv are related to cell migration, and they control cell migration by regulating the expression and function of ITGA5 and ITGB3.
Article
Oncology
Melissa Van de Gucht, Ines Dufait, Lisa Kerkhove, Cyril Corbet, Sven de Mey, Heng Jiang, Ka Lun Law, Thierry Gevaert, Olivier Feron, Mark De Ridder
Summary: This study demonstrates for the first time that modulation of de novo serine biosynthesis enhances radioresponse in hypoxic colorectal cancer cells, primarily mediated by increased intracellular ROS levels.
Article
Cell & Tissue Engineering
Marino Caruso, Damien Detraux, Amandine Mathieu, Manon Gilson, Marc Dieu, Antoine Fattaccioli, Catherine Demazy, Mustapha Najimi, Etienne Sokal, Thierry Arnould, Catherine Verfaillie, Denis L. J. Lafontaine, Patricia Renard
Summary: Translational regulation plays a crucial role in proteome remodeling during stem cell differentiation. This study investigated translational remodeling during hepatogenic differentiation of induced pluripotent stem cells (iPSCs) using polysome profiling. The findings suggest that protein synthesis increases during the initial stages of hepatogenic differentiation and is then globally repressed during later stages. However, despite the overall translational repression, key hepatogenic genes are efficiently translated and even induced for transcripts involved in hepatospecific functions and metabolic maturation.
Article
Oncology
Junjie Lan, Octavia Cadassou, Cyril Corbet, Olivier Riant, Olivier Feron
Summary: The study demonstrates the feasibility of efficiently screening for mitochondrial complex I inhibitors based on the ability of drug candidates to induce L-lactate release. Drug candidates identified showed complex I inhibitory activity and potential radiosensitizing effects in tumor-mimicking conditions, indicating promising anticancer properties.
Article
Chemistry, Analytical
Maxime Gavage, Kaatje Van Vlierberghe, Marc Dieu, Patsy Renard, Thierry Arnould, Kris Gevaert, Marc De Loose, Christof Van Poucke, Anne-Catherine Huet, Nathalie Gillard
Summary: This interlaboratory study evaluated the potential of MS for food allergen detection and quantification using a standard addition quantification strategy and a stable isotope-labeled concatemer as an internal standard. Nine laboratories participated in the study and were able to detect allergens with sufficient sensitivity, although egg detection was more challenging. The encouraging results of this study contribute to harmonization among laboratories testing for allergens.
JOURNAL OF AOAC INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Sebastien Dilly, Miguel Romero, Stephanie Solier, Olivier Feron, Chantal Dessy, Anny Slama Schwok
Summary: ROS in cancer cells play a crucial role in regulating cell death, stemness maintenance, and metabolic reprogramming, which are associated with resistance to chemo/immunotherapy. This paper introduces new inhibitors of NADPH oxidase (NOX), which is important in the tumor microenvironment. One of the inhibitors, called Nanoshutter-1 (NS1), decreases tumor-promoting M2 macrophages in human blood monocytes. NS1 disrupts the NOX2 complex in the macrophages' membrane and mitochondria, leading to vasodilation of blood vessels and potential reduction of hypoxia. By targeting specific cells, NOX2-specific inhibitors could provide precision therapies.
Article
Cell Biology
Sophie Ayama-Canden, Rodolfo Tondo, Martha Liliana Pineros Leyton, Noelle Ninane, Catherine Demazy, Marc Dieu, Antoine Fattaccioli, Aude Sauvage, Tijani Tabarrant, Stephane Lucas, Davide Bonifazi, Carine Michiels
Summary: Metastasis is the leading cause of mortality in breast cancer, especially in triple negative breast cancer. The extracellular matrix, specifically the IGDQ motif in fibronectin, plays a significant role in regulating cell migration before metastasis formation. SRSF6 is identified as a potential master regulator of cell migration and integrin trafficking, with Indacaterol showing potential as a therapy to prevent cell migration and reduce metastasis formation in breast cancer.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Sebastien Meurant, Lorris Mauclet, Marc Dieu, Thierry Arnould, Sven Eyckerman, Patricia Renard
Summary: Biotin-based proximity labeling approaches, specifically BioID, have been used to study mitochondria proteomes in living cells. Genetically engineered cell lines enable detailed characterization of poorly understood processes (e.g., mitochondrial co-translational import). However, the mechanisms are still unclear, and few actors have been identified in mammals. Using BioID, the TOM20 proxosome was profiled, revealing enrichment of RNA binding proteins, although their role in the co-translational import process was not demonstrated for selected candidates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)