Article
Chemistry, Analytical
Sean Toffessi Siewe, Samuel Calle, Francois Vander Meulen, Damien Valente, Jean-Marc Gregoire, Aline Banquart, Stephanie Chevalliot, Arnaud Capri, Franck Levassort
Summary: Tailored backings with high acoustic impedance and strong attenuation are needed for miniaturized high-frequency transducer design. The use of multiphasic metallic backings in this study has shown promising results in terms of sensitivity and bandwidth for imaging applications.
Article
Acoustics
Yasuhiko Orita, Susumu Shimanuki, Satoshi Okada, Kentaro Nakamura, Hiroyuki Nakamura, Yoshitaka Kitamoto, Yusuke Shimoyama, Yuta Kurashina
Summary: This study demonstrated the superior acoustic responsiveness of liposomes synthesized under high pressure from supercritical CO2 and irradiated with ultrasound at 237 kHz. The release efficiency of CO2-loaded liposomes was 17.1 times higher than liposomes synthesized using the conventional Bangham method. Liposomes synthesized using supercritical CO2 and mono-ethanolamine had a release efficiency that was 19.8 times higher than the conventional Bangham method. These findings suggest a new liposome synthesis strategy for on-demand drug release using ultrasound irradiation in future therapies.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Acoustics
Chi-Man Wong, Siu-Fan Chan, Wei Chang Wu, Chun-Hung Suen, Hei-Man Yau, Dan Yang Wang, Sean Li, Ji Yan Dai
Summary: A high compression fabrication method is presented in this article to tune the acoustic impedance of alumina-epoxy composite matching layer from 6.50 to 9.47 MRayl by controlling pressure and component ratios. The enhanced performance of this matching material, with very high acoustic impedance value and very low attenuation, makes it superior to all reported matching materials.
Article
Biotechnology & Applied Microbiology
Han Shan, Qibo Lin, Danfeng Wang, Xin Sun, Biao Quan, Xiang Chen, Zeyu Chen
Summary: This study presents a method for ultra-high volumetric throughput nanoliposome preparation using 3D printed microfluidic chips. By adopting a three-layer layout for microchannels, the microfluidic chip achieved a total flow rate up to 474 ml min(-1) and controlled the liposome size to be as small as 80 nm. The experimental results demonstrate the great potential of the 3D printed integrated microfluidic chip in targeting drug delivery systems.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Joseph A. Sebastian, Eric M. Strohm, Emmanuel Cherin, Bahram Mirani, Christine E. M. Demore, Michael C. Kolios, Craig A. Simmons
Summary: High-frequency ultrasound can non-invasively characterize and monitor the acoustic properties of biomaterials and engineered tissues. A new method is proposed to accurately measure the speed of sound, acoustic impedance, and acoustic attenuation of cell-laden hydrogels, taking into account the frequency-dependent effects of attenuation in coupling media, hydrogel thickness, and interfacial transmission/reflection coefficients of ultrasound waves. The acoustic attenuation coefficients were found to increase with increasing cell concentration, reflecting the cellularity of the hydrogel independent of its thickness.
ACTA BIOMATERIALIA
(2023)
Article
Acoustics
Silvia Nalesso, Gaelle Varlet, Madeleine J. Bussemaker, Richard P. Sear, Mark Hodnett, Rebeca Monteagudo-Olivan, Victor Sebastian, Joaquin Coronas, Judy Lee
Summary: A systematic study on sonocrystallisation of ZIF-8 in a water-based system was conducted, revealing that ultrasound and mixing affect crystal morphology and size, while increasing mixing speed can enhance the BET specific surface area.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Hwankyu Lee, Hyungwon Moon, Hyun-Ryoung Kim
Summary: Cholesterol-lipid interactions restrict the lateral dynamics of bilayers but promote bilayer curvature, while lyso-lipids effectively pack the vacancies in cone-shaped lipid bilayers, reducing dynamics and curvature.
Article
Acoustics
Suzi Liang, Min Su, Baoqiang Liu, Rong Liu, Hairong Zheng, Weibao Qiu, Zhiqiang Zhang
Summary: This study evaluated the influence of blood on HD-IVUS imaging and found that blood significantly affects the imaging quality, with a 71.7% decrease in signal-to-noise ratio. Additionally, the study identified dextran 5% as a suitable flushing agent for HD-IVUS due to its minimal signal attenuation.
IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
(2022)
Article
Chemistry, Analytical
Hayong Jung, K. Kirk Shung, Hae Gyun Lim
Summary: Ultrasound imaging is a valuable tool in imaging human tissues, but conventional transducers face limitations in resolution for cellular applications. By using ultrahigh frequency (UHF) transducers, we achieved high-resolution cell imaging and acoustic tweezers. The results showed successful imaging of single cells and manipulation of cells and beads using the UHF transducers.
Article
Physics, Multidisciplinary
Anton V. Nikolaev, Hendrik H. G. Hansen, Thomas J. J. Maal, Nens van Alfen, Chris L. de Korte
Summary: This study quantitatively determines the acquisition limit for image quality in free-hand volumetric ultrasound (FVUS) and analyzes the translation speeds used by sonographers. It also presents a design of fast FVUS that allows for higher frame rates and faster transducer translation while maintaining quantitative image information.
FRONTIERS IN PHYSICS
(2022)
Article
Medicine, General & Internal
Li Li, Jiaosheng Xu, Siwei Wang, Jun Yang
Summary: PM is a heterogeneous, well-demarcated, hypoechoic tumor located between the deep dermis and the subcutis on ultra-high-frequency ultrasonography. The most common features are internal echogenic foci (calcifying) and hypoechoic rim. Calcifying types can help in the staging of PM. Ultra-high-frequency ultrasound is a useful tool for the diagnosis and evaluation of PM.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Applied
Yue Wu, Wu Li, Gregory J. O. Martin, Muthupandian Ashokkumar
Summary: The study found that 20 kHz and 355 kHz ultrasound had better inactivation efficiency on soy trypsin inhibitors, with increasing ultrasonic power leading to higher inactivation rate constants. For 20 kHz ultrasound, the formation of TI aggregates decreased TI activity, while for 355 kHz ultrasound, oxidation of methionine in TIs accelerated inactivation. Aggregation of TIs further contributed to TI inactivation, with SDS-PAGE showing no involvement of disulphide bonds or C-C coupling in the process.
Article
Food Science & Technology
Yanhua Ding, Haile Ma, Ke Wang, S. M. Roknul Azam, Yaoyao Wang, Jie Zhou, Wenjuan Qu
Summary: This study developed a method to predict the resonance frequencies in soybean protein extraction by simulating the acoustic field distribution. Experimental results showed that ultrasound significantly improved the extraction rate of soybean protein, with 28 kHz being the optimal frequency. Spectral analysis indicated that certain frequencies had a significant impact on the structure of protein molecules.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Neurosciences
Subeen Kim, Yehhyun Jo, Geun Ho Im, Chanhee Lee, Chaerin Oh, Geon Kook, Seong-Gi Kim, Hyunjoo J. Lee
Summary: Visualization of focused ultrasound using high-resolution 9.4 T MRI is crucial for precise brain targeting. However, limitations such as small RF volume coil size and noise sensitivity of external systems hinder high-resolution MR imaging in small animal studies. This technical note presents a miniaturized ultrasound system integrated with MR-compatible materials and electromagnetic noise reduction techniques, allowing visualization of ultrasound-induced effects in mouse brain using high-resolution 9.4 T MRI. The proposed system enables extensive research in ultrasound therapeutics.
Article
Polymer Science
Abhishek Ranjan, Chengxiang Peng, Sanat Wagle, Frank Melandso, Anowarul Habib
Summary: This study compares the image quality of a low-cost copolymer P(VDF-TrFE) transducer with a commercial PVDF transducer using a scanning acoustic microscope. Various test objects were examined and image quality was evaluated in terms of resolution, signal-to-noise ratio, point-spread function, and depth imaging capability. Useful information about the strengths and limitations of low-cost polymer transducers is believed to be provided by this investigation.
Article
Engineering, Chemical
Satomi Hirose, Jean-Paul Rieu, Olivier Cochet-Escartin, Christophe Anjard, Kenichi Funamoto
Summary: Spatiotemporal variations of oxygen concentration impact cell behaviors involved in physiological and pathological events. For Dd cells, oxygen-dependent characteristics affect their migration properties, such as migration frequency, velocity, and direction, depending on oxygen levels and gradients.
Article
Biology
Noriko Tomita, Sho Saito, Junko Terada-Hirashima, Ayako Mikami, Yukari Uemura, Satoshi Kutsuna, Hidetoshi Nomoto, Kyoko Fujisawa, Maki Nagashima, Mari Terada, Shinobu Ashida, Shinichiro Morioka, Masahiro Satake, Akira Hangaishi, Tomiteru Togano, Katsuyuki Shiratori, Yuki Takamatsu, Kenji Maeda, Norio Ohmagari, Wataru Sugiura, Hiroaki Mitsuya
Summary: This study aims to evaluate the efficacy of convalescent plasma from recovered COVID-19 patients. It will compare the convalescent plasma group to a standard-of-care group to assess the changes in viral load and other measures. The study hopes to provide further evidence for the role of convalescent plasma therapy in COVID-19.
Article
Oncology
Radhika Mishra, Ariunbuyan Sukhbaatar, Arunkumar Dorai, Shiro Mori, Kiyoto Shiga, Tetsuya Kodama
Summary: Treating metastatic lymph nodes is challenging due to poor drug uptake. A study found that altering the physical environment of lymph nodes by changing osmotic pressure and viscosity can improve drug retention and enhance therapeutic efficacy.
Article
Acoustics
Anam Bhatti, Takuro Ishii, Naoya Kanno, Hayato Ikeda, Kenichi Funamoto, Yoshifumi Saijo
Summary: Observing alterations in cutaneous vasculature can be a potential diagnostic marker for diseases. High-frequency ultrasound (HFUS) has been used in dermatology to observe skin morphologies and tissue conditions, but its ability to visualize micro-vessels is limited. In this study, a novel region-based SVD filtering approach was proposed to visualize cutaneous vascular networks. Experimental observations showed that this approach improved the image quality and has potential applications in the diagnosis of cutaneous disorders.
Article
Acoustics
Muhammad Shiddiq Sayyid Hashuro, Simon Tupin, Narendra Kurnia Putra, Kotaro Daibo, Kosuke Inoue, Takuro Ishii, Hiroyuki Kosukegawa, Kenichi Funamoto, Toshiyuki Hayase, Makoto Ohta
Summary: A transparent ultrasound flow phantom material composed of poly(vinyl alcohol) hydrogel (PVA-H) with dimethyl sulfoxide (DMSO) and water solution was proposed for visualizing and measuring flow inside ultrasound systems. The material's refractive index was modified to match that of glass for transparency. Feasibility tests were conducted, and an ultrasound flow phantom was fabricated for B-mode visualization and Doppler-PIV comparison. The results showed promising accuracy in velocity measurement and similarity to real tissue visualization, with some limitations regarding sound velocity and Doppler measurements.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Naoyuki Takahashi, Daisuke Yoshino, Ryuji Sugahara, Satomi Hirose, Kazuki Sone, Jean-Paul Rieu, Kenichi Funamoto
Summary: This study developed a microfluidic platform for reconstructing hypoxic vascular microenvironments, by simultaneously applying hypoxic stress and fluid shear stress. The results showed that under the simultaneous exposure to hypoxic stress and fluid shear stress, the migration velocity of endothelial cells increased and gradually decreased, reaching the lowest value. Meanwhile, the cells aligned and elongated in the flow direction, with enhanced expression of VE-cadherin and actin filament assembly. Therefore, this microfluidic platform is useful for investigating the dynamics of endothelial cells in vascular microenvironments.
SCIENTIFIC REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Suguru Miyauchi, Koichi Hosoi, Shingo Tsuda, Toshiyuki Hayase, Kenichi Funamoto
Summary: This study investigated the effects of complex internal structures and torsional motion on left ventricular hemodynamics using computational fluid dynamics analyses. The presence of trabeculae carneae and papillary muscles decreased average wall shear stress and increased the relative residence time of blood stagnation at the apex of the left ventricle. Torsional motion caused irregular changes to the relative residence time level and distribution at the apex. Therefore, the complex internal structures and torsional motion of the left ventricle could cause blood stagnation.
Article
Cell Biology
Satomi Hirose, Julie Hesnard, Nasser Ghazi, Damien Roussel, Yann Voituron, Oliver Cochet-Escartin, Jean-Paul Rieu, Christophe Anjard, Kenichi Funamoto
Summary: Spatial and temporal variations of oxygen environments affect cell behaviors, and the aerotaxis of Dictyostelium discoideum seems to be an effective strategy for survival. Flavohemoglobins, potential oxygen sensors and oxidative stress modulators, play a role in this phenomenon.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Noriko Tomita, Eriko Morino, Junko Terada-Hirashima, Yukari Uemura, Yosuke Shimizu, Sho Saito, Tetsuya Suzuki, Nobumasa Okumura, Haruka Iwasaki, Hideki Ebihara, Masayuki Shimojima, Wataru Sugiura, Norio Ohmagari, Mugen Ujiie
Summary: This study aims to investigate the immunogenicity and safety of the LC16KMB vaccine in healthy Japanese adults. The primary endpoint is the seroconversion rate of neutralizing antibodies against the monkeypox virus, and secondary endpoints include seroconversion rates against the vaccinia virus, incidence of monkeypox, and adverse events. These results will help pave the way for larger comparative studies to evaluate the safety and efficacy of other smallpox vaccines in preventing monkeypox.
Article
Biotechnology & Applied Microbiology
Noriko Tomita, Junko Terada-Hirashima, Yukari Uemura, Yosuke Shimizu, Haruka Iwasaki, Rina Yano, Tetsuya Suzuki, Sho Saito, Nobumasa Okumura, Wataru Sugiura, Norio Ohmagari, Mugen Ujiie
Summary: Monkeypox, caused by the monkeypox virus, has become a community-acquired infection spreading in Europe, the United States, and Japan. A study was conducted to investigate the post-exposure prophylaxis of monkeypox and the safety of smallpox vaccine inoculation. Participants who had close contact with monkeypox patients were inoculated with Freeze-dried cell culture Smallpox Vaccine LC16 within 14 days. No monkeypox symptoms or complications were observed in the participants for 21 days. Adverse events due to inoculation were non-severe and non-serious, and all participants recovered within the observation period.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)
Article
Engineering, Biomedical
Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Ayumu Konno, Hirokazu Hirai, Taku Saito, Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa, Takamasa Sakai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura, Kenichi Funamoto, Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao, Yasuhiro Sawada
Summary: Vertically oscillating head motions can reduce blood pressure in hypertensive individuals, possibly by reducing the expression of angiotensin II type-1 receptor in astrocytes. This suggests that mechanical interventions could be used to elicit antihypertensive effects.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Health Care Sciences & Services
Rina Yano, Junko Terada-Hirashima, Yukari Uemura, Noriko Tomita, Yosuke Shimizu, Haruka Iwasaki, Nobumasa Okumura, Tetsuya Suzuki, Sho Saito, Mugen Ujiie, Wataru Sugiura, Norio Ohmagari
Summary: A study aims to evaluate the efficacy and safety of the LC16m8 strain vaccine for postexposure prophylaxis against monkeypox. 100 vaccinated participants who had close contact with monkeypox patients were enrolled, and their symptoms and side effects were recorded for 21 days. The study results will be published in a scientific journal.
JMIR RESEARCH PROTOCOLS
(2023)
Meeting Abstract
Peripheral Vascular Disease
Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Masahiro Shinohara, Motoshi Nagao, Yasuhiro Sawada
JOURNAL OF HYPERTENSION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Yasuhiro Sawada, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Ayumu Konno, Hirokazu Hirai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao
Review
Engineering, Biomedical
Makoto Ohta, Naoya Sakamoto, Kenichi Funamoto, Zi Wang, Yukiko Kojima, Hitomi Anzai
Summary: Vascular endothelial cells are exposed to mechanical stress from blood flow and their functions can be studied using specific chamber designs. The functions of these cells are closely connected to the outer layers of arteries and organs, and the interaction between smooth muscle cells in the middle layer and endothelial cells can be investigated using coculture chambers. Additionally, microfabrication technology in microfluidic devices allows for mechanical and chemical stimulation of cells to study their dynamics in vivo microenvironments.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)