Article
Chemistry, Analytical
Xinmei Liu, Bo Xie, Yongjian Cheng, Lin Luo, Yifan Liang, Zhili Xiao
Summary: This study developed a sensitive icELISA method for detecting CPPU, which has high affinity and specificity for CPPU, and is suitable for rapid detection of CPPU in food samples.
Article
Chemistry, Applied
Yang Liu, Xinxin Xu, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu
Summary: In this study, a GNP-based lateral flow immunoassay method was developed for the sensitive and specific detection of isoprothiolane (IPT) in rice. The method showed a low detection limit and comparable recovery rates to LC-MS/MS.
Article
Biophysics
Keine Nishiyama, Kazuki Takahashi, Mao Fukuyama, Motohiro Kasuya, Ayuko Imai, Takumi Usukura, Nako Maishi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Kyoko Hida, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Summary: This study presents an analytical method using fluorescence polarization immunoassay (FPIA) for rapid and quantitative detection of SARS-CoV-2 antibody in human serum. The FPIA system showed high accuracy in discriminating COVID-19 positive or negative serum samples. The total measurement time was about 20 minutes, and a serum volume of 0.25 μL was required, making it a rapid and convenient method for quantification of SARS-CoV-2 antibody.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Lianrun Huang, Fuxuan Zhang, Fangxuan Li, Yin Jia, Minghua Wang, Xiude Hua, Limin Wang
Summary: The established ic-ELISA and LFIA methods proved to be accurate and rapid for the determination of cypermethrin (CYP) in agricultural products.
Article
Food Science & Technology
Lingyuan Xu, A. M. Abd El-Aty, Jae-Han Shim, Jong-Bang Eun, Xingmei Lei, Jing Zhao, Xiuyuan Zhang, Xueyan Cui, Yongxin She, Fen Jin, Lufei Zheng, Jing Wang, Maojun Jin, Bruce D. Hammock
Summary: This study developed a design and synthetic protocol for preparing highly sensitive and specific ATR monoclonal antibodies, which exhibited high specificity, sensitivity, and affinity.
Article
Chemistry, Applied
Peng Wang, Xinxin Xu, Shanshan Song, Aihong, Wu, Liqiang Liu, Hua Kuang, Chuanlai Xu
Summary: A highly sensitive monoclonal antibody was developed to detect clomazone in potato and pumpkin samples using a gold nanoparticle-based lateral flow immunochromatographic strip. The method showed good specificity and reliability, making it a potentially valuable tool for on-site and rapid initial screening of clomazone.
Article
Food Science & Technology
Yang Liu, Xinxin Xu, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu
Summary: A new hapten was designed to develop a highly sensitive and specific anti-penconazole monoclonal antibody (mAb), and a gold nanoparticle-based lateral-flow immunoassay (LFIA) was established for the rapid detection of penconazole residues in watermelon and cucumber.
FOOD QUALITY AND SAFETY
(2022)
Article
Food Science & Technology
Yang Liu, Xinxin Xu, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu
Summary: A highly sensitive and specific anti-PEN monoclonal antibody was developed in this study, along with a gold nanoparticle-based LFIA strip for detecting PEN residues in watermelon and cucumber. The results obtained using the LFIA strip were highly consistent with those seen using liquid chromatography-tandem mass spectrometry.
FOOD QUALITY AND SAFETY
(2022)
Article
Chemistry, Applied
Yuan Cheng, Aihong Wu, Lingling Guo, Maozhong Sun, Rui Gao, Hua Kuang, Chuanlai Xu, Liguang Xu
Summary: We discovered ZOX-hapten by introducing a carboxyl extension chain and generated a monoclonal antibody against ZOX. Using the generated antibody, we developed a LFIA strip to detect ZOX residues in grape, tomato, and cucumber samples. The LFIA strip showed a low detection limit and high recovery rates, indicating its potential for rapid screening of ZOX residues in fruits and vegetables.
Article
Chemistry, Applied
Peng Dai, Yan Zhang, Yanping Hong, Jianhua Xiong, Huaying Du, Luying Duan, Dan Wang, Yubo Wang, Weijie Deng, Bruce D. Hammock, Wuying Yang
Summary: A sensitive and reliable icCLEIA method was developed to detect gentamicin residues in animal tissues. By preparing an anti-gentamicin monoclonal antibody and utilizing immunological analysis techniques, gentamicin can be detected rapidly and accurately.
Article
Food Science & Technology
Shuangmin Wu, Huaming Li, Xiaoyang Yin, Yu Si, Liangni Qin, Hongfei Yang, Jiaxu Xiao, Dapeng Peng
Summary: An indirect competitive enzyme-linked immunoassay (ic-ELISA) was established to detect PYR and BaP residues in living aquatic products for the first time, with high sensitivity and accuracy.
Article
Plant Sciences
Kaewta Rattanapisit, Tharita Kitisripanya, Atthaphon Konyanee, Worapol Sae-Foo, Apisit Burapapiruin, Waraporn Putalun, Seiichi Sakamoto, Waranyoo Phoolcharoen, Gorawit Yusakul
Summary: The plant expression platform is a new source for producing IgG targeting small low-molecular-weight molecules. The study demonstrated that plant-produced functional IgG maintained the binding activity and specificity of IgG derived from mammalian cells, showing potential as an efficient and cost-effective platform for antibody production in immunoassays targeting low-molecular-weight compounds.
PLANT CELL REPORTS
(2021)
Article
Chemistry, Analytical
Beijun Ang, Xinxin Xu, Liguang Xu, Liqiang Liu, Hua Kuang, Chuanlai Xu
Summary: In this study, a method combining enzymatic hydrolysis with a colloidal gold lateral flow immunoassay (LFIA) was established to detect total homocysteine (tHcy) in blood, which has the potential to predict cardiovascular risk accurately.
Article
Chemistry, Applied
Rongzhi Wang, Juncheng Wang, Haimei Liu, Yehong Gao, Qiang Zhao, Sumei Ling, Shihua Wang
Summary: The study identified a specific IgG against bovine LF for rapid detection of LF content in cow milk using ic-ELISA and lateral flow immunosensors, without the need for expensive equipment and sample pretreatment.
Article
Chemistry, Applied
Xianlu Lei, Xinxin Xu, Li Wang, Liqiang Liu, Hua Kuang, Liguang Xu, Chuanlai Xu
Summary: In this study, a sensitive monoclonal antibody (mAb) 1B5 against eugenols was prepared and used for the development of a paper-based lateral-flow immunoassay (LFIA) using Eu-fluorescent microspheres sensor. The LFIA showed promising results for qualitative and quantitative detection of eugenols within 10 minutes. The recovery test and detection in fish samples demonstrated the reliability of the LFIA in real samples.
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)
