Article
Oncology
Giovanni Nicolao Berta, Federica Di Scipio, Zhiqian Yang, Alessandra Oberto, Giuliana Abbadessa, Federica Romano, Maria Elisabetta Carere, Adriano Ceccarelli, Emilio Hirsch, Barbara Mognetti
Summary: The study revealed that the lack or inhibition of PI3K gamma can delay preneoplastic and exophytic lesions in oral carcinogenesis. WT mice showed higher expression of prognostic markers and certain cell types, while tongues isolated from transgenic mice had more abundant T lymphocytes. A specific PI3K gamma inhibitor significantly impaired the vitality of oral cancer cells.
Article
Food Science & Technology
Mariaurea Matias Sarandy, Silvania Mol Pelinsari, Lorena Miranda de Souza, Romulo Dias Novaes, Virginia Vinha Zanuncio, Reggiani Vilela Goncalves
Summary: We evaluated the healing of cutaneous wounds in male wild type (WT) and knockout (KO) mice (iNOS) after supplementation with L-citrulline (CIT) and L-arginine (ARG). The wound area was smaller and the contraction rate was higher with ARG and CIT in WT and KO. ARG and CIT accelerated healing and tissue repair at morphological, histological and biochemical levels.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Anatomy & Morphology
E. Aloni, S. Verbitsky, L. Kushnireva, E. Korkotian, M. Segal
Summary: Research indicates that Synaptopodin (SP) plays a key role in the spine apparatus of central excitatory neurons and the axon initial segment. Lack of SP in SPKO mice results in cognitive deficiencies, increased excitability in hippocampal cells, and higher expression of arc staining.
BRAIN STRUCTURE & FUNCTION
(2021)
Article
Biochemistry & Molecular Biology
Ken Ichiro Tanaka, Sachie Shiota, Okina Sakakibara, Mikako Shimoda, Ayaka Takafuji, Misaki Takabatake, Yoshito Kadota, Takashige Kawakami, Shinya Suzuki, Masahiro Kawahara
Summary: Metallothionein plays an important role in the development of chronic obstructive pulmonary disease (COPD) by suppressing elastase-induced lung injury through the inhibition of reactive oxygen species production.
Article
Oncology
Natalia Krupenko, Jaspreet Sharma, Halle M. Fogle, Peter Pediaditakis, Kyle C. Strickland, Xiuxia Du, Kristi L. Helke, Susan Sumner, Sergey A. Krupenko
Summary: The loss of ALDH1L1 promotes liver tumor growth without affecting tumor initiation or multiplicity. Knockout mice lacking ALDH1L1 showed faster growth of larger tumors, with differences in metabolic pathways linked to rapid proliferation.
Article
Virology
Chen Li, Wen-Feng He, Long-Xi Li, Jing Chen, Guo-Qing Yang, Hong-Tao Chang, Hui-Min Liu
Summary: ISG15 plays a crucial role in the host antiviral response, as demonstrated by its knockout leading to increased susceptibility to PRV infection and loss of antiviral protection. ISG15 deficiency results in reduced expression of IFN beta and relevant ISGs, and excessive production of proinflammatory cytokines may contribute to pathological damage.
Article
Immunology
Lihui Lin, Ying Li, Gaoshi Zhou, Ying Wang, Li Li, Jing Han, Minhu Chen, Yao He, Shenghong Zhang
Summary: This study aimed to analyze the impact of a Western-style diet (WSD) on experimental colitis susceptibility and its underlying mechanism using transcriptomics and metabolomics. The results showed that WSD induced more severe inflammation in experimental colitis and identified differential genes and metabolites related to the diet. Integrating transcriptomic and metabolomic data revealed major co-expression networks through which WSD promoted experimental IBD susceptibility.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Biology
Veronika Lang, Sebastian Ferencik, Bharath Ananthasubramaniam, Achim Kramer, Bert Maier
Summary: Local circadian clocks play a role in the body's immune response, with light affecting susceptibility to endotoxic shock. Conditional knockout mice showed rhythmic patterns of immune response even without a myeloid clock. This suggests that systemic time cues can orchestrate immune functions.
Article
Neurosciences
Evgeniya V. Efimova, Alena A. Kozlova, Valeria Razenkova, Nataliia V. Katolikova, Kristina A. Antonova, Tatyana D. Sotnikova, Natalia S. Merkulyeva, Aleksandr S. Veshchitskii, Daria S. Kalinina, Dmitry E. Korzhevskii, Pavel E. Musienko, Evgeny V. Kanov, Raul R. Gainetdinov
Summary: TAAR5 is not only involved in the regulation of emotional status, but also adult neurogenesis and dopamine transmission. TAAR5-KO mice showed an increased number of proliferating neurons in the major neurogenic areas, suggesting that future TAAR5 antagonists may provide new treatment opportunities for neurodegenerative disorders like Parkinson's disease.
Article
Biochemistry & Molecular Biology
Yuriko Tanaka, Mayu Onozato, Tetuo Mikami, Terumi Kohwi-Shigematsu, Takeshi Fukushima, Motonari Kondo
Summary: The study found that early signs of Sjogren's Syndrome were observed in SATB1cKO mice, with IFN-dependent IDO expression induction being a crucial early event in the disease. Serum L-KYN could potentially be used as a marker for early SS diagnosis before autoantibodies become detectable.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Bernadetta Pawlicka, Michal Duliban, Mateusz Zieba, Michal Bochenek, Kamila Zieba, Ibrahim Adham, Maja Studencka-Turski, Andreas Meinhardt, Pawel Grzmil
Summary: Overexpression of Pxt1 gene leads to male germ cell degeneration and infertility in mice. Knockout of Pxt1 gene results in increased sperm DNA fragmentation index (DFI) without affecting fertility. The findings suggest a role for Pxt1 gene in eliminating male germ cells with chromatin damage.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Yoko Yoshihisa, Mati Ur Rehman, Tsugunobu Andoh, Yoshiaki Tabuchi, Teruhiko Makino, Tadamichi Shimizu
Summary: The study showed that D-DT Tg mice developed tumors more rapidly than wild-type mice under chronic UVB exposure, with a higher incidence of tumors observed in D-DT Tg mice. The expression of p53, PUMA, and Bax in keratinocytes of D-DT Tg mice was lower compared to wild-type mice, indicating that D-DT overexpression may confer protection against UVB-induced apoptosis in keratinocytes. These findings suggest that D-DT plays a crucial role in photocarcinogenesis and could be a potential therapeutic target for preventing skin malignancies.
Article
Pharmacology & Pharmacy
Binbin Song, Shigeki Aoki, Cong Liu, Takeshi Susukida, Saki Kuwahara, Kousei Ito
Summary: Based on studies using a transgenic mouse model, it was found that severe abacavir-induced skin hypersensitivity could be induced by depleting PD1 and CD4 T cells, which led to CD8 T cell activation and dendritic cell maturation.
Article
Pharmacology & Pharmacy
Qing-Yuan Zhong, Qi-Hong Luo, Bing Lin, Bao-Qin Lin, Zi-Ren Su, Janis Ya-Xian Zhan
Summary: The research found that andmgrapholide sodium bisulfate (ASB) can effectively inhibit the occurrence of UV-induced skin cancer through inhibiting NF-kappa B and Nrf2 signaling pathways and restoring autophagy.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Dermatology
Ai Yoshioka, Hirofumi Nakaoka, Takeshi Fukumoto, Ituro Inoue, Chikako Nishigori, Makoto Kunisada
Summary: Non-melanoma skin cancer (NMSC) is primarily caused by UV-induced somatic mutations with distinct UV signature modifications. Xpa knockout mice show extreme sensitivity to UV-induced photo-skin carcinogenesis and exhibit a photosensitive phenotype. In this study, the differences in somatic mutation landscapes between Xpa knockout and wild-type mice were investigated after repetitive UVB exposure. We found that although the tumors in both types of mice had UV signature mutations in similar cancer-related genes, the pattern of transcriptional strand asymmetry differed significantly, indicating a deficiency in transcription-coupled nucleotide excision repair in Xpa knockout mice.
EXPERIMENTAL DERMATOLOGY
(2022)
Meeting Abstract
Dermatology
J. Manils, H. Fischer, J. Climent, E. Casas, J. Bas, T. Vavouri, J. de Anta, E. Tschachler, L. Eckhart, C. Soler
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2017)
Article
Multidisciplinary Sciences
Joan Manils, Heinz Fischer, Joan Climent, Eduard Casas, Celia Garcia-Martinez, Jordi Bas, Supawadee Sukseree, Tanya Vavouri, Francisco Ciruela, Josep Maria de Anta, Erwin Tschachler, Leopold Eckhart, Concepcio Soler
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Xavier Morato, Francisco Q. Goncalves, Joao P. Lopes, Olga Jauregui, Concepcio Soler, Victor Fernandez-Duenas, Rodrigo A. Cunha, Francisco Ciruela
JOURNAL OF NEUROCHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Carlo Matera, Alexandre M. J. Gomila, Nuria Camarero, Michela Libergoli, Concepcio Soler, Pau Gorostiza
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Biochemistry & Molecular Biology
Brian Flood, Joan Manils, Ciara Nulty, Ewelina Flis, Sinead Kenealy, Gillian Barber, Joanna Fay, Kingston H. G. Mills, Elaine W. Kay, Emma M. Creagh
Letter
Dermatology
Sinead Kenealy, Joan Manils, Mathilde Raverdeau, Natalia Munoz-Wolf, Gillian Barber, Alex Liddicoat, Ed C. Lavelle, Emma M. Creagh
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2019)
Article
Biology
Joan Manils, Louise Webb, Ashleigh Howes, Julia Janzen, Stefan Boeing, Anne M. Bowcock, Steven C. Ley
Article
Neurosciences
Marta Valle-Leon, Luis F. Callado, Ester Aso, Maria M. Cajiao-Manrique, Kristoffer Sahlholm, Marc Lopez-Cano, Concepcio Soler, Xavier Altafaj, Masahiko Watanabe, Sergi Ferre, Victor Fernandez-Duenas, Jose M. Menchon, Francisco Ciruela
Summary: According to the adenosine hypothesis of schizophrenia, a hypoadenosinergic state may lead to a reduction in A(2A)R-D2R heteromers, which could be a hallmark of the illness. The study suggests that chronic antipsychotic treatments might influence the formation of A(2A)R-D2R heteromers and warrants further investigation into possible correlations with schizophrenia.
NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hector Godoy-Marin, Romain Duroux, Kenneth A. Jacobson, Concepcio Soler, Hildegard Colino-Lage, Veronica Jimenez-Sabado, Jose Montiel, Leif Hove-Madsen, Francisco Ciruela
Summary: Atrial fibrillation (AF) is influenced by various factors such as oxidative stress, calcium overload, inflammation, as well as adenosine and its receptors. Studies have found increased A2AR expression in the right atrium of AF patients, elevated levels of adenosine content in plasma, and reduced ADA activity. Importantly, there is a positive correlation between A2AR expression in PBMCs and in the right atrium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Laura Marruecos, Joan Manils, Cristina Moreta, Diana Gomez, Ingrid Filgaira, Anna Serafin, Xavier Canas, Lluis Espinosa, Concepcio Soler
Summary: Loss of a single Trp53 allele can impact the oxidative, DNA damage, and inflammatory status of hematopoietic lineages, potentially facilitating and supporting carcinogenesis over time.
CELL DEATH & DISEASE
(2021)
Article
Pharmacology & Pharmacy
Marc Lopez-Cano, Ingrid Filgaira, Ernest G. Nolen, Gisela Cabre, Jordi Hernando, Dilip K. Tosh, Kenneth A. Jacobson, Concepcio Soler, Francisco Ciruela
Summary: Psoriasis is a chronic inflammatory skin disease affecting approximately 2% of the population, with defective keratinocyte proliferation and differentiation, along with aberrant immune responses being major factors in its pathogenesis. Current treatments for moderate to severe psoriasis may lead to systemic immunosuppression and serious side effects, such as infections and cancer. In recent years, the A(3) receptor for adenosine has emerged as a promising therapeutic target for psoriasis, showing robust anti-inflammatory effects in animal models of autoimmune inflammatory diseases.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Cell Biology
Joan Manils, Laura Marruecos, Concepcio Soler
Summary: DNA degradation plays a crucial role in maintaining genomic stability and cellular homeostasis. Exonucleases, enzymes capable of removing nucleotides from the end of a DNA chain, are involved in vital cellular processes such as DNA replication, repair, and immune response regulation. Dysfunctions in these enzymes are associated with immune disorders and cancer.
Article
Biochemistry & Molecular Biology
Xavier Llorente, Gerard Esteruelas, Lorena Bonilla, Mariana Garnica Agudelo, Ingrid Filgaira, Daniel Lopez-Ramajo, Ruoyi C. Gong, Concepcio Soler, Marta Espina, Maria Luisa Garcia, Joan Manils, Montserrat Pujol, Elena Sanchez-Lopez
Summary: Nanocarriers, including nanostructured lipid carriers (NLC), are highly effective for topical drug administration. Optimized NLCs loaded with the drug Riluzole (RLZ-NLCs) were developed as a potential treatment, featuring biodegradability, biocompatibility, and sustained drug release. RLZ-NLCs showed successful delivery of RLZ in a sustained manner, inhibition of keratinocyte cell proliferation, and non-angiogenic properties, making them a promising strategy for treating keratinocyte hyperproliferative conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)