Article
Pharmacology & Pharmacy
Marta Zimna, Gabriela Brzuska, Jiri Salat, Pavel Svoboda, Klaudia Baranska, Boguslaw Szewczyk, Daniel Ruzek, Ewelina Krol
Summary: Tick-borne encephalitis virus (TBEV) causes severe neurological infections. Dairy products from infected animals are a common cause. Vaccination of livestock could help reduce human infections. Researchers developed a potential TBEV vaccine based on virus-like particles (VLPs) produced in Leishmania tarentolae cells, which proved to be highly immunogenic and provided full protection from lethal TBE in mice.
ANTIVIRAL RESEARCH
(2023)
Article
Cell Biology
XiaoLuan Xu, AnQi Ma, TieSong Li, WenXue Cui, XueFeng Wang, Jun Li, Qingwei Li, Yue Pang
Summary: Research on lampreys has identified brown-like adipocytes in the brain periphery, similar to human BAT, that play a role in generating heat, adapting to low temperatures, and enhancing inflammation response. This provides new insights for understanding the function and characteristics of BAT in humans, as well as the adaptation mechanisms of lampreys in cold environments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Ben Jones, Vinod Burade, Elina Akalestou, Yusman Manchanda, Zenouska Ramchunder, Gaelle Carrat, Marie-Sophie Nguyen-Tu, Piero Marchetti, Lorenzo Piemonti, Isabelle Leclerc, Rajamannar Thennati, Tina Vilsboll, Bernard Thorens, Alejandra Tomas, Guy A. Rutter
Summary: This study describes the in vitro characteristics and antidiabetic efficacy of GL0034, a novel GLP-1RA. GL0034 showed increased binding affinity and bias towards cAMP signaling compared to semaglutide. In vivo studies in mice demonstrated that GL0034 had powerful antidiabetic effects, including weight loss and improved glycemic control.
DIABETES OBESITY & METABOLISM
(2022)
Article
Biotechnology & Applied Microbiology
Jian Zhang, Wanyuan Dong, Yuhong Ren, Dongzhi Wei
Summary: In this study, a novel recombinant fusion protein, SAC-(G(4)S)(3)-TRAIL, was successfully generated and shown to exhibit enhanced antitumor activity and efficient and specific binding to cancer cells. These findings suggest that this fusion protein holds potential as a therapeutic agent for cancer.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Wenliang Zhou, Yuan Zhang, Shixi Zhang, Zongguo Yang
Summary: Identifying potential biomarkers for predicting overall survival in HCC patients, this study found AIM1L to be significantly upregulated in tumor tissues and associated with unfavorable OS. This novel finding suggests AIM1L may serve as a valuable predictor for prognosis in HCC patients.
Article
Biochemistry & Molecular Biology
Sergey A. Vishnivetskiy, Liana D. Weinstein, Chen Zheng, Eugenia V. Gurevich, Vsevolod V. Gurevich
Summary: Arresin-1 shows selectivity for light-activated phosphorylated rhodopsin, and this selectivity is mediated by two structural elements in the molecule. In the crystal structure, there are some residues close to rhodopsin that do not belong to the selectivity mechanism. Mutations in these residues enhance binding to unphosphorylated rhodopsin and increase its selectivity for phosphorylated rhodopsin. This challenges the current model of arrestin-receptor interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Xi Lin, Junwan Lu, Changrui Qian, Hailong Lin, Qiaoling Li, Xueya Zhang, Hongmao Liu, Zhewei Sun, Danying Zhou, Wei Lu, Mei Zhu, Hailin Zhang, Teng Xu, Kewei Li, Qiyu Bao, Li Lin
Summary: An increasing antibiotic resistance in Aeromonas hydrophila isolates has been observed, with a lack of in-depth studies on the role of plasmids in this resistance. Investigation into a multidrug-resistant plasmid encoding an NDM-like metallo-beta-lactamase, AFM-1, in the clinical A. hydrophila isolate SS332 revealed high levels of resistance to multiple antibiotics. The functional verification of a novel blaNDM-like gene, bla(AFM-1), and its association with novel ISCR19-like elements highlights the risk of spread of these resistance genes.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Biotechnology & Applied Microbiology
Ying He, Shanshan Chen, Kexin Liu, Yongji Chen, Yanhao Cheng, Peng Zeng, Peiwen Zhu, Ting Xie, Sunlu Chen, Hongsheng Zhang, Jinping Cheng
Summary: In this study, we identified a new gene OsHIPL1 as a causal gene for rice seed vigour. Our findings suggest that OsHIPL1 regulates seed vigour in rice by modulating endogenous ABA levels and altering the expression of ABA-related genes. Furthermore, we found that OsHIPL1 interacts with the aquaporin OsPIP1;1, affecting water uptake and promoting rice seed germination.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Elaheh Mohebbi, Eleonora Pavoni, Davide Mencarelli, Pierluigi Stipa, Luca Pierantoni, Emiliano Laudadio
Summary: This study thoroughly investigated the properties of VO2(B) polymorph using density functional theory calculations. The combination of GGA/PBEsol and HSE hybrid functionals was found to accurately describe its electronic and optical characteristics. The findings suggest the potential of VO2(B) in electronics and optoelectronics applications and highlight the importance of accurate modeling in smart materials.
Article
Plant Sciences
Shoukun Chen, Kunjie Li, Bin Tan, Yaning Wei, Haifeng Li
Summary: In this study, nine PYL genes were identified in the Brachypodium distachyon genome, which are distributed on three chromosomes. These genes are expressed at high levels in leaves and their expression is regulated by ABA and drought. Overexpression of one of the genes, BdPYL5, in Arabidopsis enhanced ABA sensitivity and drought tolerance, indicating its conserved function. This study provides a foundation for further functional elucidation of BdPYL genes.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Yung-Tuen Chiu, Abdullah Husain, Karen Man-Fong Sze, Daniel Wai-Hung Ho, Eliana Mary Senires Suarez, Xia Wang, Eva Lee, Hoi-Tang Ma, Joyce Man-Fong Lee, Lo-Kong Chan, Irene Oi-Lin Ng
Summary: This study identified Mid-line 1 interacting protein 1 (MID1IP1) as a key gene in the metastasis of hepatocellular carcinoma (HCC). It was found that MID1IP1 promoted HCC migration and invasion by upregulating FOS like 1 (FRA1) and subsequently activating MMP9 signaling. Targeting the MID1IP1-mediated FRA1 pathway may be a potential therapeutic strategy against HCC progression.
Article
Oncology
Samrita Dogra, Sugantha Priya Elayapillai, Dongfeng Qu, Kamille Pitts, Alexander Filatenkov, Courtney W. Houchen, William L. Berry, Katherine Moxley, Bethany N. Hannafon
Summary: Ovarian cancer (OvCa) has a poor prognosis due to late stage diagnosis and chemoresistance. DCLK1, a serine/threonine kinase, plays a role in regulating cancer cell stemness, epithelial-mesenchymal transition (EMT), and drug resistance. Inhibition of DCLK1 can increase sensitivity to cisplatin and reduce cell proliferation, migration, and invasion. High DCLK1 expression is associated with poor overall and progression-free survival in ovarian cancer patients. Targeting DCLK1 in combination with existing chemotherapy may be a novel therapeutic approach to overcome resistance and prevent recurrence.
Article
Multidisciplinary Sciences
Lara Ribeiro-Parenti, Anne-Charlotte Jarry, Jean-Baptiste Cavin, Alexandra Willemetz, Johanne Le Beyec, Aurelie Sannier, Samira Benadda, Anne-Laure Pelletier, Muriel Hourseau, Thibaut Leger, Bastien Morlet, Anne Couvelard, Younes Anini, Simon Msika, Jean-Pierre Marmuse, Severine Ledoux, Maude Le Gall, Andre Bado
Summary: This study reveals the presence of GLP-1 expressing cells in the stomach, which secrete active GLP-1 and are influenced by weight loss surgeries. Gastric glucose load induces a rapid increase in GLP-1 levels, and obese rats and individuals who undergo surgery exhibit changes in gastric mucosa phenotype.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Ningjie Cui, Shang Li, Yonglei Zhang, Fucheng Yin, Xinye Chen, Zhongwen Luo, Siyuan Wan, Xinxin Li, Lingyi Kong, Xiaobing Wang
Summary: This study synthesized and evaluated a series of structural analogues of Sibiriline and found that the optimal compound KWCN-41 can inhibit cell death, protect cell survival, and reduce inflammation levels. It is expected to be a potential drug for treating inflammatory diseases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Pediatrics
Huixian Wang, Gaoli Jiang, Dan Dai, Da Hong, Weitao Zhou, Liling Qian
Summary: In this study, two novel NKX2-1 frameshift variants were identified in two unrelated children. In vitro experiments revealed common and different mechanisms for these variants, partially explaining the phenotypical heterogeneity.
PEDIATRIC RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Fa-An Chao, Srisathiyanarayanan Dharmaiah, Troy Taylor, Simon Messing, William Gillette, Dominic Esposito, Dwight Nissley, Frank McCormick, R. Andrew Byrd, Dhirendra K. Simanshu, Gabriel Cornilescu
Summary: This article investigates the influence of conformational dynamics of RAS proteins on their interactions with downstream effectors and explains and predicts the altered binding affinities of different mutants.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Multidisciplinary Sciences
Jessica Hong, Hyung Joon Kwon, Raul Cachau, Catherine Z. Chen, Kevin John Butay, Zhijian Duan, Dan Li, Hua Ren, Tianyuzhou Liang, Jianghai Zhu, Venkata P. Dandey, Negin P. Martin, Dominic Esposito, Uriel Ortega-Rodriguez, Miao Xu, Mario J. Borgnia, Hang Xie, Mitchell Ho
Summary: The study constructed large dromedary camel VHH phage libraries and successfully isolated two high-affinity nanobodies that showed broad neutralization activity against SARS-CoV-2 and its variants. These nanobodies can be used for therapeutic purposes against COVID-19 variants, and the dromedary camel VHH phage libraries serve as a unique platform for rapidly isolating potent nanobodies against future emerging viruses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Kyungeun Kim, Kris Ylaya, Candice Perry, Mi-Yeon Lee, Jeong Won Kim, Joon-Yong Chung, Stephen M. Hewitt
Summary: Water is a crucial factor for protein and RNA degradation in stored tissue sections, and storage conditions can affect the expression of certain key proteins and RNA integrity in breast tissue.
BIOPRESERVATION AND BIOBANKING
(2023)
Article
Surgery
Kapil K. Saharia, Sabrina C. Ramelli, Sydney R. Stein, Allison E. Roder, Allie Kreitman, Stephanie Banakis, Joon-Yong Chung, Peter D. Burbelo, Manmeet Singh, Robert M. Reed, Vipul Patel, Joseph Rabin, Alexander S. Krupnick, Jeffrey I. Cohen, Emmie de Wit, Elodie Ghedin, Stephen M. Hewitt, Kevin M. Vannella, Daniel S. Chertow, Alison Grazioli
Summary: Although the risk of SARS-CoV-2 transmission through lung transplantation from acutely infected donors is high, using lungs from COVID-19-recovered donors does not significantly increase the risks of virus transmission and long-term lung allograft outcomes.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Pathology
H. Nida Sen, Kevin M. Vannella, Yujuan Wang, Joon-Yong Chung, Shilpa Kodati, Sabrina C. Ramelli, Jung Wha Lee, Paola Perez, Sydney R. Stein, Alison Grazioli, James M. Dickey, Kris Ylaya, Manmeet Singh, Kwe Claude Yinda, Andrew Platt, Marcos J. Ramos-Benitez, Christa Zerbe, Vincent J. Munster, Emmie de Wit, Blake M. Warner, Daniel L. Herr, Joseph Rabin, Kapil K. Saharia, David E. Kleiner, Stephen M. Hewitt, Chi-Chao Chan, Daniel S. Chertowt
Summary: This study evaluated the pathology and cellular localization of SARS-CoV-2 in ocular tissues through autopsy. It found histopathologic abnormalities in ocular tissues and identified the localization of SARS-CoV-2 RNA in multiple cell types. Further studies are needed to determine the cause of these alterations.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Joo Mi Yi, Tae-Hong Kang, Yu Kyeong Han, Ha Young Park, Ju Hwan Yang, Jin-Han Bae, Jung-Soo Suh, Tae-Jin Kim, Joong-Gook Kim, Yan-Hong Cui, Hiromu Suzuki, Kohei Kumegawa, Sung Joo Kim, Yi Zhao, In Ja Park, Seung-Mo Hong, Joon-Yong Chung, Su-Jae Lee
Summary: This study identifies Neuralized (NEURL) as a novel tumor suppressor gene that targets oncogenic Wnt/beta-catenin signaling in human cancers. NEURL expression is epigenetically regulated and suppressed in colorectal cancer. NEURL acts as an E3 ubiquitin ligase, interacting with oncogenic beta-catenin and reducing its cytoplasmic levels, thereby disrupting the canonical Wnt/beta-catenin pathway.
Article
Cell Biology
Joon-Yong Chung, Kyungeun Kim, Kris Ylaya, Katharine E. Walker-Bawa, Candice Perry, Robert A. Star, Stephen M. Hewitt
Summary: Neutral buffered formalin (NBF) is commonly used as a fixative in clinical applications, but it damages proteins and nucleic acids, limiting the quality of molecular analysis. Previous studies have shown that BE70, a fixative of buffered 70% ethanol, has advantages over NBF, but protein and nucleic acid degradation in archival paraffin blocks is still a challenge. With the addition of guanidinium salts, BE70G fixative improves the quality of molecular analysis, provides better protein and RNA quality at shorter fixation times, and reduces protein degradation in archival tissue blocks.
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
(2023)
Article
Oncology
Gwan Hee Han, Hee Yun, Joon-Yong Chung, Jae-Hoon Kim, Hanbyoul Cho
Summary: The study aimed to investigate the function, molecular mechanisms, and clinical significance of ATP6V1B1 in epithelial ovarian cancer (EOC). It was found that ATP6V1B1 was highly expressed in EOC tissues and was associated with clinicopathological features and prognosis. Knockdown of ATP6V1B1 inhibited cancer cell proliferation and colony-forming abilities. Therefore, ATP6V1B1 could serve as a biomarker for prognostic evaluation and chemotherapy resistance, as well as a potential therapeutic target in EOC.
Article
Multidisciplinary Sciences
Se Jin Oh, Ji Yeon Lim, Min Kyu Son, Jun Hyeok Ahn, Kwon-Ho Song, Hyo-Jung Lee, Suyeon Kim, Eun Ho Cho, Joon-Yong Chung, Hanbyoul Cho, Hyosun Kim, Jae-Hoon Kim, Jooyoung Park, Jungmin Choi, Sun Wook Hwang, Tae Woo Kim
Summary: The emergence of cisplatin resistance and side-effects are important factors in cancer treatment failure. The authors identify autophagy-mediated EGFR hyperactivation by TRPV1 promotion as a mechanism of cisplatin resistance and demonstrate the efficacy of a TRPV1 inhibitor to sensitize lung cancer to cisplatin. Future studies should provide evidence regarding the mechanisms of potential targets that can overcome the resistance as well as alleviate pain.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Ha Kyun Chang, Young Hoon Park, Jung-A Choi, Jeong Won Kim, Jisup Kim, Hyo Sun Kim, Hae Nam Lee, Hanbyoul Cho, Joon-Yong Chung, Jae-Hoon Kim
Summary: The expression of Nectin-4 is significantly increased in endometrial cancer and can be used as a diagnostic marker. High Nectin-4 expression is associated with DNA mismatch repair protein deficiency and may predict short progression-free survival. This study provides clues for selecting patients for adjuvant therapy.
Article
Oncology
Ji-Won Ryu, Ha-Yeon Shin, Hyo-Sun Kim, Gwan Hee Han, Jeong Won Kim, Hae-Nam Lee, Hanbyoul Cho, Joon-Yong Chung, Jae-Hoon Kim
Summary: Hormonal factors, particularly steroid hormones, are believed to play a significant role in ovarian cancer development. The expression of glucocorticoid receptor (GR) has been associated with poor prognosis in epithelial ovarian cancer. This study investigates the clinical significance of beta-arrestin expression in ovarian cancer and the potential synergistic effect with GR as prognostic factors. The findings demonstrate a moderate correlation between beta-arrestin 1 and GR expression, suggesting a possible role in prognostication.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Razaul Haque, Jaebon Lee, Joon-Yong Chung, Ha-Yeon Shin, Hyosun Kim, Jae-Hoon Kim, Jae Won Yun, Eun-Suk Kang
Summary: This study suggests that VGLL3 is associated with tumor development and prognosis in patients with high-grade serous ovarian carcinoma, as well as with tumor-infiltrating macrophages. VGLL3 may serve as a potential prognostic marker and therapeutic target for ovarian cancer.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Jung-A Choi, Hyosun Kim, Hyunja Kwon, Elizabeth Hyeji Lee, Hanbyoul Cho, Joon-Yong Chung, Jae-Hoon Kim
Summary: This study examined the clinical value and therapeutic potential of ascitic autotaxin (ATX) in epithelial ovarian cancer (EOC). The results showed that elevated levels of ascitic ATX were associated with advanced stage, high grade, and a short disease-free period in EOC patients. Combining ascitic ATX and serum CA-125 levels improved the diagnostic accuracy for EOC, and functional studies revealed that ascitic ATX played a role in maintaining cancer stem cell-like characteristics and invadopodia formation in EOC.
BRITISH JOURNAL OF CANCER
(2023)
Article
Medicine, Research & Experimental
Jordan M. White, Nicholas Ramos, Allen-Dexter Saliganan, Joon-Yong Chung, Meghan Bell, Jacob Lindquist, Kayla Conner, Wendy N. Wiesend, Michael Schopperle, Steve M. Patrick, Seongho Kim, Elisabeth I. Heath, Freddy E. Escorcia, Nerissa T. Viola
Summary: This study investigated the clinical implications of TRA expression in prostate cancer. It found that high PODXL copy number alterations were associated with poor prognosis. TRA-targeted PET imaging successfully located cancer stem cells (CSCs) and TRA-targeted RPT showed promising therapeutic effects. Ablation of TRA+ CSCs inhibited prostate cancer growth.