Journal
CANCER LETTERS
Volume 312, Issue 2, Pages 228-234Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2011.08.009
Keywords
Quenching probe; Mutation-biased PCR; Bcr-abl; Mutation; Leukemia
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Funding
- Japan Society for the Promotion of Science (JSPS)
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- Government to the National Cancer Centre of Japan
- Grants-in-Aid for Scientific Research [21390293, 23592678, 21591246, 23591404] Funding Source: KAKEN
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ABL tyrosine kinase inhibitor (TKI), imatinib is used for BCR-ABL(+) leukemias. We developed an automatic method utilizing guanine-quenching probes (QP) to detect 17 kinds of mutations frequently observed in imatinib-resistance. Results were obtained from 100 mu L of whole blood within 90 min by this method. Detected mutations were almost identical between QP method and direct sequencing. Furthermore, the mutation-biased PCR (MBP) was added to the QP method to increase sensitivity, resulting earlier detection of T315I mutation which was insensitive to any ABL TKIs. Thus, the QP and MBP-QP may become useful methods for the management of ABL TKI-treated patients. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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