Journal
CANCER LETTERS
Volume 268, Issue 2, Pages 278-285Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2008.04.002
Keywords
Erc gene; Tsc2; siRNA microspheres
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Ministry of Health, Labor and Welfare of Japan
- Japan Society
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)
- Government of Japan
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Silencing of gene expression by small interfering RNAs (siRNAs) is rapidly becoming a powerful tool for genetic analysis and represents a potential strategy for therapeutic product development. However, there are no reports of systemic delivery of siRNAs for stable treatment except short hairpin RNAs (shRNAs). On the other hand, there are no reports of systemic delivery of siRNAs for transient treatment using liposome carriers and others. With regard to shRNAs, a report showed fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Therefore, we decided to use original siRNA microspheres instead of shRNA for stable treatment of disease. In this study, we designed rat-specific siRNA sequences for Erc/mesothelin, which is a tumor-specific gene expressed in the Eker (Tsc2 mutant) rat model of hereditary renal cancer and confirmed the efficacy of gene silencing in vitro. Then, by using siRNA cells in tumor implantation experiments in mice. (c) 2008 Elsevier Ireland Lts. All rights reserved.
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