Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 758, Issue -, Pages 20-25Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2015.10.010
Keywords
Electrochemical biosensor; MicroRNA; Hybridization chain reaction; Signal amplification; Streptavidin
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Funding
- Special Fund Project for Key Laboratory of Clinical Laboratory Diagnostics, Chongqing Medical University
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MicroRNAs (miRNAs) play vital roles in regulating gene expression and cellular processes. Dysregulation of miRNAs has been associated with a variety of diseases, which make them promising biomarkers. In this work, a simple and ultrasensitive electrochemical biosensor was developed for detection of target miRNA using DNA-based hybridization chain reaction (HCR) and biotin-strepavidin based amplification. In the presence of target miRNA, the hairpin DNA capture probes immobilized on gold electrode surface were opened. Then DNA initiator strands hybridized with the opened capture probes and triggered HCR, finally, a long nicked double-helix DNA structure was developed. Streptavidin is employed for signal amplification via binding with biotin labeled on the two hairpin DNA probes which take part in the HCR. The proposed biosensor presented a high sensitivity for determination of target miRNA down to 0.56 fM with a linear range from 1 fM to 100 pM and high specificity that discriminates target miRNA from mismatched miRNAs. The method was successfully applied to the determination of miRNA spiked into human total RNA samples. Therefore, the biosensor might have potential clinical application for miRNA detection in biomedical research and clinical diagnosis. (C) 2015 Elsevier B.V. All rights reserved.
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