Article
Gastroenterology & Hepatology
Stefania Bunduc, Bianca Varzaru, Razvan Andrei Iacob, Andrei Sorop, Ioana Manea, Andreea Spiridon, Raluca Chelaru, Adina Emilia Croitoru, Gabriel Becheanu, Mona Dumbrava, Simona Dima, Irinel Popescu, Cristian Gheorghe
Summary: This study assessed the adequacy of DNA yielded from EUS-FNA pancreatic adenocarcinoma (PDAC) samples for next generation sequencing (NGS). The results showed that DNA concentration and purity in EUS-FNA samples were suitable for NGS. There was no significant difference in DNA yield between 22G and 19G needles, but DNA purity parameters may vary with needle size.
WORLD JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Endocrinology & Metabolism
Xiao-qin Qian, Enock Adjei Agyekum, Ling-ling Zhao, Run-liu Yu, Xiu-ying Li, De-jian Gu, Na Yan, Ming Xu, Yuan Yuan, Yu-guo Wang, Xin-ping Wu, Fei-ju Xu
Summary: This study compared the performance of DP-TOF and NGS methods in detecting multiple-gene mutations in thyroid nodule fine-needle aspiration fluid. The MS method demonstrated higher sensitivity and specificity in detecting BRAF(V600E) gene mutation compared to NGS. The MS method can be considered as an inexpensive, accurate, and dependable initial screening method to detect gene mutations.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Oncology
Celia Dupain, Julien Masliah-Planchon, Celine Gu, Elodie Girard, Pierre Gestraud, Pauline Du Rusquec, Edith Borcoman, Diana Bello, Francesco Ricci, Segolene Hescot, Marie-Paule Sablin, Patricia Tresca, Alexandre de Moura, Delphine Loirat, Maxime Frelaut, Anne Vincent-Salomon, Charlotte Lecerf, Celine Callens, Samantha Antonio, Coralie Franck, Odette Mariani, Ivan Bieche, Maud Kamal, Christophe Le Tourneau, Vincent Servois
Summary: This study aimed to evaluate the potential of fine-needle aspiration (FNA) compared to core needle biopsies (CNB) in detecting molecular alterations in various tumor types, with a high concordance of 80% between FNA and CNB. Comparative analysis showed that FNA and CNB have similar high concordance in terms of variants and copy number variations (CNVs) identified.
MOLECULAR ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Beate Haugk, David Horton, Kofi Oppong, John Leeds, Antony Darne, Philip Sloan, Thomas Ness, Claire Jones, Paul Bassett, Manu Nayar
Summary: The study assessed p40 expression in fine needle biopsy samples of PDAC and found it to be associated with squamous differentiation in PDAC. For inoperable patients, a p40 H-score > 30 may indicate a shorter survival time. Therefore, squamous differentiation could be a potential treatment target in PDAC.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Yoshiki Naito, Masayuki Tsuneki, Noriyoshi Fukushima, Yutaka Koga, Michiyo Higashi, Kenji Notohara, Shinichi Aishima, Nobuyuki Ohike, Takuma Tajiri, Hiroshi Yamaguchi, Yuki Fukumura, Motohiro Kojima, Kenichi Hirabayashi, Yoshihiro Hamada, Tomoko Norose, Keita Kai, Yuko Omori, Aoi Sukeda, Hirotsugu Noguchi, Kaori Uchino, Junya Itakura, Yoshinobu Okabe, Yuichi Yamada, Jun Akiba, Fahdi Kanavati, Yoshinao Oda, Toru Furukawa, Hirohisa Yano
Summary: In this study, a deep learning model was trained to assess PDAC on EUS-FNB specimens of the pancreas in histopathological whole-slide images with high accuracy. The model was able to accurately detect isolated and low volume cancer cells, showing potential as a supportive system in routine diagnosis of pancreatic EUS-FNB specimens.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Chia-Sheng Chu, Chi-Ying Yang, Chun-Chieh Yeh, Ro-Ting Lin, Chi-Ching Chen, Li-Yuan Bai, Mien-Chie Hung, Chun-Che Lin, Chun-Ying Wu, Jaw-Town Lin
Summary: Investigating the intra-tumoral microbiome through endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) can provide adequate fresh pancreatic cancer tissue for research. This study found that the microbiome profiles obtained from EUS-FNB and surgical biopsy were comparable in terms of alpha-diversity and taxonomic composition.
SCIENTIFIC REPORTS
(2022)
Article
Gastroenterology & Hepatology
Joseph R. Habib, Yayun Zhu, Lingdi Yin, Ammar A. Javed, Ding Ding, Jonathan Tenior, Michael Wright, Syed Z. Ali, Richard A. Burkhart, William Burns, Christopher L. Wolfgang, Eunji Shin, Jun Yu, Jin He
Summary: Targeted next-generation sequencing (NGS) can detect KRAS mutations from EUS-FNA samples, showing high concordance with primary tumors and ctDNA. This suggests that targeted NGS of EUS-FNA samples may enhance diagnostic accuracy in PDAC and enable preoperative ctDNA prognostication.
JOURNAL OF GASTROINTESTINAL SURGERY
(2021)
Article
Biochemistry & Molecular Biology
Martin Lovecek, Pavel Skalicky, Ondrej Urban, Jana Tesarikova, Martin Kliment, Robert Psar, Hana Svebisova, Katerina Urban, Beatrice Mohelnikova-Duchonova, Dusan Klos, Martin Stasek
Summary: Endosonography-guided fine needle aspiration biopsy (EUS-FNA)-associated metachronous gastric seeding metastases (GSM) of pancreatic ductal adenocarcinoma (PDAC) can be preventable and curable, requiring follow-up and multimodal management.
Review
Biology
Masataka Taniuchi, Tetsuya Terada, Ryo Kawata
Summary: Fine-needle aspiration cytology is widely used for preoperative evaluation of parotid tumors, with core needle biopsy and liquid-based cytology improving reliability of immunostaining and molecular testing.
Article
Oncology
In Ae Kim, Jae Young Hur, Hee Joung Kim, Song Am Lee, Jae Joon Hwang, Wan Seop Kim, Kye Young Lee
Summary: Genetic analysis of 131 EGFR-mutated lung adenocarcinoma patients revealed associations between co-mutations and specific mutation subtypes with recurrence rate and prognosis, providing guidance for identifying high-risk patients and guiding treatment.
Article
Endocrinology & Metabolism
Dongyan Han, Min Ding, Rongli Xie, Zhengshi Wang, Guohui Xiao, Xiaohong Wang, Lei Dong, Zhiqiang Yin, Jian Fei
Summary: This study examined the genetic mutation profile of thyroid nodules and evaluated the diagnostic ability of molecular testing. The results showed that cytology and molecular testing had high diagnostic abilities in Bethesda II and V-VI samples, while further analysis of genetic mutations was needed for accurate prediction of malignant nodules in Bethesda III-IV nodules.
ENDOCRINE CONNECTIONS
(2023)
Review
Medicine, General & Internal
Masaki Kuwatani, Kazumichi Kawakubo, Naoya Sakamoto
Summary: The poor prognosis of biliary tract cancer is due to the challenges in detecting cancer lesions and the lack of standard screening methods. Genomic analysis has shown promise in improving the accuracy of diagnosis for biliary tract cancer, and future diagnosis and treatment should be based on molecular and genetic analysis.
Article
Oncology
Roberto Chalela, Jose Gregorio Gonzalez-Garcia, Karys Khilzi, Victor Curull, Albert Sanchez-Font, Raquel Longaron, Maria Teresa Rodrigo-Calvo, Clara Martin-Ontiyuelo, Joaquim Gea, Beatriz Bellosillo
Summary: Driver mutations in non-tumoral lung cells were detected in a proportion of subjects with ADC, indicating their potential role in carcinogenesis. The presence of these mutations in the absence of coexisting mutations in the primary tumor was confirmed through highly sensitive PCR methods.
PATHOLOGY & ONCOLOGY RESEARCH
(2021)
Article
Multidisciplinary Sciences
Saif Bunni, Heikki J. Nieminen
Summary: This study investigates the impact of needle bevel geometry and length on resonance characteristics and deflection magnitude, finding differences in deflection-to-power ratio among needles with different bevel geometries in air and water. The study emphasizes the importance of the relationship between bevel geometry flexural stiffness and insertion media, providing insights for controlling post-puncture cutting action.
SCIENTIFIC REPORTS
(2022)
Article
Endocrinology & Metabolism
Michiya Nishino, Roselyn Mateo, Holly Kilim, Anna Feldman, Amanda Elliott, Changyu Shen, Per-Olof Hasselgren, Helen Wang, Pamela Hartzband, James V. Hennessey
Summary: Repeat FNA cytology can guide the selection of indeterminate thyroid nodules for molecular testing, reducing unnecessary surgical risks for benign nodules while only missing a few low-risk tumors.