Journal
CANCER CELL INTERNATIONAL
Volume 14, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12935-014-0120-x
Keywords
Secretomic; Glycoproteomic; Two dimensional electrophoresis; N-glycoprotein; Concanavalin A lectin
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Funding
- UMRG grants from University of Malaya [RG454-12HTM]
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Background: Concurrent study of secretomic and glycoproteomic profiles in cancer cell lines represents an excellent approach for investigating cancer progression and identifying novel biomarker candidates. In this study, we performed a comparative secretomic and N-glycoprotein profiling from the secretions of normal human mammary epithelial cells (HMEpC) and the MCF-7 human breast cancer cell line. Method: We analyzed these cell lines using a combined methodology involving glycan-binding lectins and two-dimensional electrophoresis and identified several differentially secreted factors, including osteonectin and haptoglobin. Result: Notably, comparative analyses also revealed that MCF-7 cells produced differentially N-glycosylated forms of haptoglobin. Conclusion: The present data suggested that osteonectin and haptoglobin might have potential to be served as potential biomarkers for breast cancer. However, further investigation is needed to validate the findings.
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