Article
Biotechnology & Applied Microbiology
Yuan Zhang, Xiaofeng Lai, Qingfang Yue, Fei Cao, Yue Zhang, Yang Sun, Jun Tian, Yizhao Lu, Li He, Jun Bai, Yifang Wei
Summary: This study revealed that BMSCs-derived exosomal miR-16-5p can inhibit the growth and metastasis of breast cancer cells through modulation of the EPHA1/NF-kappa B signaling pathway.
Article
Plant Sciences
Gege Xu, Haiping Zhao, Jingdong Xu, Yu Zhang, Xiaoyan Qi, Aiping Shi
Summary: The research indicates that HAE can effectively inhibit self-renewal of stem-like cells, wound healing, and metastasis in breast cancer cells by targeting the NF-kappa B signaling pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Cell & Tissue Engineering
Jia Sun, Xuan Sun, Junhui Chen, Xin Liao, Yixuan He, Jinsong Wang, Rui Chen, Sean Hu, Chen Qiu
Summary: The study revealed that miR-27b highly expressed in exosomes derived from MSCs decreased the expression of pro-inflammatory genes by inhibiting the recruitment of JMJD3 and NF-kappa B, thereby reducing the production of pro-inflammatory cytokines in septic mice and LPS-treated BMDMs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Pharmacology & Pharmacy
Huang-Chi Chen, Hsin-Ying Clair Chiou, Mei-Lan Tsai, Szu-Chia Chen, Ming-Hong Lin, Tzu-Chun Chuang, Chih-Hsing Hung, Chao-Hung Kuo
Summary: This study demonstrates that montelukast is effective at reducing arsenic-induced epithelial-mesenchymal transition (EMT) in human bronchial epithelial cells. By inhibiting arsenic-induced reactive oxygen species (ROS) generation and NF-kB activation, montelukast can suppress arsenic-induced cell migration and the expression of extracellular matrix proteins and EMT-regulating transcription factors. Combination treatment with fluticasone and montelukast can reverse the inhibitory effect of montelukast on arsenic-induced EMT.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Juliane Strietz, Stella S. Stepputtis, Marie Follo, Peter Bronsert, Elmar Stickeler, Jochen Maurer
Summary: Primary breast cancer stem cell (BCSC) cultures isolated from TNBC specimens have been established and shown to grow as tumor spheres under anchorage-independent culture conditions in vitro, while reliably forming tumors in mice when transplanted in limiting dilutions in vivo. Additionally, these BCSC xenograft tumors exhibit similar architecture and gene expression to the original patient tumor, suggesting a promising model for researching BCSC biology and testing new treatment options for TNBC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Lixian Yang, Shuo Liu, Liu Yang, Bin Xu, Meiqi Wang, Xiangshun Kong, Zhenchuan Song
Summary: miR-217-5p expression is decreased while MTDH expression is increased in breast cancer tissues. Overexpression of miR-217-5p can inhibit cell proliferation, migration, and epithelial-mesenchymal transition in breast cancer cells by suppressing the NF-kappa B signaling pathway. Furthermore, there is a negative correlation between miR-217-5p and MTDH, and restoration of MTDH expression levels can attenuate the inhibitory effects of miR-217-5p overexpression on breast cancer cell proliferation.
Article
Cell Biology
Yang Zhang, Weixing Ni, Lei Qin
Summary: In this study, the researchers aimed to investigate the role and mechanism of RUFY3 in hepatocellular carcinoma (HCC) progression. Results showed that high expression of RUFY3 was significantly associated with tumor size, microvascular invasion, clinical stage, and poor prognosis in HCC patients. Furthermore, RUFY3 was found to promote HCC cell growth, invasion, and metastasis through activating NF-kappa B-mediated epithelial-mesenchymal transition (EMT). These findings suggest that RUFY3 may serve as a novel target for HCC treatment.
Article
Biochemistry & Molecular Biology
Luis Arturo Palafox-Mariscal, Pablo Cesar Ortiz-Lazareno, Luis Felipe Jave-Suarez, Adriana Aguilar-Lemarroy, Maria Martha Villasenor-Garcia, Jose Roberto Cruz-Lozano, Karen Lilith Gonzalez-Martinez, Anibal Samael Mendez-Clemente, Alejandro Bravo-Cuellar, Georgina Hernandez-Flores
Summary: Cervical cancer is a common and deadly type of female cancer. Inflammation can lead to tumor metastasis and EMT. The anti-inflammatory compound PTX may disrupt EMT and reduce cell migration and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Marketa Koleckova, Jiri Ehrmann, Jan Bouchal, Maria Janikova, Aneta Brisudova, Josef Srovnal, Katerina Staffova, Marek Svoboda, Ondrej Slaby, Lenka Radova, Katherine Vomackova, Bohuslav Melichar, Lucia Veverkova, Zdenek Kolar
Summary: Triple negative breast cancers (TNBC) are a morphologically and genetically heterogeneous group of breast cancers with uncertain prediction of biological behavior and response to therapy. Epithelial to mesenchymal transition (EMT) is a dynamic process characterized by loss of typical epithelial phenotype and acquisition of mesenchymal characteristics. The study aimed to analyze miRNA expression within areas of TNBCs with cellular morphology related to the EMT process, and found a specific miRNA expression profile of apocrine and spindle cell morphology which may be relevant for prognosis and therapy resistance of TNBC.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Zepeng Xu, Ni Tian, Songtao Li, Kunmeng Li, Haike Guo, Hongyang Zhang, Haiying Jin, Meixia An, Xiaoyi Yu
Summary: This study investigated the anti-inflammatory and anti-apoptotic effects of hUCMSC-sEVs in DR, showing a reduction in inflammation in rat retinas and predicting miR-18b as a key factor.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Huahua Tu, Wei Wang, Yanqing Feng, Linfei Zhang, Huadong Zhou, Caitao Cheng, Lei Ji, Qinghe Cai, Yong Feng
Summary: Beta-patchoulene (beta-PAE) is a compound extracted from patchouli with analgesic, anti-inflammatory, and antioxidant effects. This study found that beta-PAE can inhibit the growth, proliferation, migration, and epithelial-mesenchymal transition of hypoxia-induced hepatocellular carcinoma (HCC) cells, and promote apoptosis.
Article
Biochemistry & Molecular Biology
Alessia Paganelli, Francesca Diomede, Guya Diletta Marconi, Jacopo Pizzicannella, Thangavelu Soundara Rajan, Oriana Trubiani, Roberto Paganelli
Summary: Galectin-3 (GAL-3) inhibits the expression of TLR4, NF-κB p65, MyD88, and NALP3 in LPS-stimulated hGMSCs, and this inhibitory effect can be reversed by GAL-3 BP or GAL-3 antibody.
Review
Oncology
Barbora Kvokackova, Jan Remsik, Mohit Kumar Jolly, Karel Soucek
Summary: Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) play critical roles in the development of TNBC, shaping aggressive and heterogeneous biological features, but may also contribute to the progression of metastatic disease.
Article
Multidisciplinary Sciences
Wenhua Shao, Natsumi Fujiwara, Yasuhiro Mouri, Satoru Kisoda, Kayo Yoshida, Kaya Yoshida, Hiromichi Yumoto, Kazumi Ozaki, Naozumi Ishimaru, Yasusei Kudo
Summary: Research shows that Fusobacterium nucleatum can induce the conversion of epithelial phenotype to p-EMT phenotype in oral squamous cell carcinoma cells by altering gene expression, promoting metastasis.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Qiannan Zhao, Fen Jiang, Hui Zhuang, Yanfeng Chu, Fang Zhang, Chenghong Wang
Summary: miR-124-3p inhibits HCC proliferation and epithelial-mesenchymal transition (EMT) by targeting ARRDC1.
Article
Oncology
Emily Latacz, Pieter-Jan Van Dam, Christian Vanhove, Laura Llado, Benedicte Descamps, Nuria Ruiz, Ines Joye, Dirk Grunhagen, Steven Van Laere, Piet Dirix, David G. Mollevi, Cornelis Verhoef, Luc Dirix, Peter Vermeulen
Summary: The histopathological growth patterns (HGPs) of liver metastases can predict patient outcomes and resistance to systemic treatment, but their full potential has not been realized. Medical imaging studies show promise in identifying HGPs, but further research is needed to confirm their accuracy and utility in treatment planning.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Oncology
Carole Mercier, Michael Claessens, Andy Buys MSc, Sergii Gryshkevych, Charlotte Billiet, Ines Joye, Steven Van Laere, Peter Vermeulen, Paul Meijnders, Fredrik Lofman, Philip Poortmans, Luc Dirix, Dirk Verellen, Piet Dirix
Summary: The study aimed to evaluate the efficacy of SABR for non-spine bone and lymph node metastases and investigate the impact of different dose fractionations. Results showed that all three treatment schedules were feasible and effective with significantly low toxicity rates and high local control rates.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Oncology
Steffi Oeyen, Vincent Liegeois, Bram De Laere, Andy Buys, Michiel Strijbos, Piet Dirix, Paul Meijnders, Peter Vermeulen, Steven Van Laere, Luc Dirix
Summary: Automated CTC enumeration is reliable for prognosis assessment in mCRPC patients, and studying CTC phenotypic diversity adds value to patient prognostication beyond enumeration. High CTC phenotypic diversity before treatment is associated with shorter progression-free survival and overall survival, highlighting the importance of considering this factor in patient care.
PROSTATE CANCER AND PROSTATIC DISEASES
(2021)
Article
Cardiac & Cardiovascular Systems
Isabel Witvrouwen, Andreas B. Gevaert, Nadine Possemiers, Paul J. Beckers, Anne Vorlat, Hein Heidbuchel, Steven J. Van Laere, Amaryllis H. Van Craenenbroeck, Emeline M. Van Craenenbroeck
Summary: Exercise training is an effective therapy for patients with heart failure with reduced ejection fraction, however, around 55% of patients do not improve their VO(2)peak. This study identified a panel of seven miRNAs that are highly correlated with VO(2)peak trainability, which may help predict training response and guide individualized exercise prescription for patients.
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2021)
Article
Oncology
Charlotte Rypens, Francois Bertucci, Pascal Finetti, Fredika Robertson, Sandra Fernandez, Naoto Ueno, Wendy A. Woodward, Kenneth Van Golen, Peter Vermeulen, Luc Dirix, Patrice Viens, Daniel Birnbaum, Gayathri R. Devi, Massimo Cristofanilli, Steven Van Laere
Summary: In this study, the transcriptome of a series of inflammatory breast cancer (IBC) preclinical models was evaluated and compared to patient samples. It was found that the IBC preclinical models are predominantly estrogen receptor-negative and of the basal-like subtype, which is similar to the molecular characteristics observed in patients. The study also revealed important roles for cell proliferation, MYC transcriptional activity, and TNF alpha/NF kappa B in IBC biology.
Article
Medicine, Research & Experimental
Raymond Barnhill, Steven van Laere, Peter Vermeulen, Sergio Roman-Roman, Sophie Gardrat, Samar Alsafadi, Malcy Tarin, Gabriel Champenois, Andre Nicolas, Alexandre Matet, Nathalie Cassoux, Vincent Servois, Manuel Rodrigues, Richard Scolyer, Alexander Lazar, Emanuela Romano, Sophie Piperno-Neumann, Pascale Mariani, Claire Lugassy
Summary: This study investigates the replacement and desmoplastic histopathological growth patterns (HGP) in uveal melanoma liver metastases (MUM). The presence of L1CAM and a laminin vascular network is associated with the aggressive replacement HGP phenotype. The adverse effect of replacement HGP on overall survival and metastasis-free survival is significant, suggesting its importance in understanding tumor spread mechanisms.
LABORATORY INVESTIGATION
(2022)
Article
Oncology
Muthana Al Abo, Larisa Gearhart-Serna, Steven Van Laere, Jennifer A. Freedman, Steven R. Patierno, Eun-Sil Shelley Hwang, Savitri Krishnamurthy, Kevin P. Williams, Gayathri R. Devi
Summary: The study identified differential expression of ASR genes in aggressive breast cancer variants among different breast cancer subtypes, which were associated with the survival of African American and White breast cancer patients.
Article
Oncology
Francois Richard, Maxim De Schepper, Marion Maetens, Sophia Leduc, Edoardo Isnaldi, Tatjana Geukens, Karen Van Baelen, Ha-Linh Nguyen, Peter Vermeulen, Steven Van Laere, Francois Bertucci, Naoto Ueno, Luc Dirix, Giuseppe Floris, Elia Biganzoli, Christine Desmedt
Summary: In this study, the genomic alterations in primary and metastatic tumor samples from patients with metastatic inflammatory breast cancer (IBC) and non-IBC were compared. Higher frequency of AURKA amplification was observed in IBC, which was associated with increased AURKA mRNA expression and worse prognosis. These findings should be further investigated considering the existence of AURKA inhibitors.
Article
Oncology
Christophe Deben, Edgar Cardenas de la Hoz, Maxim Le Compte, Paul Van Schil, Jeroen M. H. Hendriks, Patrick Lauwers, Suresh Krishan Yogeswaran, Filip Lardon, Patrick Pauwels, Steven Van Laere, Annemie Bogaerts, Evelien Smits, Steve Vanlanduit, Abraham Lin
Summary: A high-throughput and automated live-cell image analysis software called OrBITS was developed for kinetic monitoring of organoids. The software combines computer vision with convolutional network machine learning and was validated against standard assays. Using OrBITS, the drug screening of lung and pancreatic cancer treatments provided further insights into the drugs' mechanism of action.
Article
Multidisciplinary Sciences
Xiaoping Wang, Takashi Semba, Ganiraju C. Manyam, Jing Wang, Shan Shao, Francois Bertucci, Pascal Finetti, Savitri Krishnamurthy, Lan Thi Hanh Phi, Troy Pearson, Steven J. Van Laere, Jared K. Burks, Evan N. Cohen, James M. Reuben, Fei Yang, Hu Min, Nicholas Navin, Van Ngu Trinh, Toshiaki Iwase, Harsh Batra, Yichao Shen, Xiang Zhang, Debu Tripathy, Naoto T. Ueno
Summary: This study reveals that EGFR-targeted therapy can remodel the tumor microenvironment of inflammatory breast cancer, leading to increased cytotoxic T cells and reduced immunosuppressive cells, thereby improving treatment efficacy. Additionally, induction of immunoactivation by an EGFR antibody enhances the antitumor effect of immune checkpoint inhibitors.
Article
Cell Biology
Guerman Molostvov, Mariam Gachechiladze, Abeer M. Shaaban, Steven Hayward, Isaac Dean, Irundika H. K. Dias, Nahla Badr, Irini Danial, Fiyaz Mohammed, Vera Novitskaya, Liliia Paniushkina, Valerie Speirs, Andrew Hanby, Irina Nazarenko, David R. Withers, Steven van Laere, Heather M. Long, Fedor Berditchevski
Summary: The immune microenvironment in breast cancer is regulated by cancer cell-derived extracellular vesicles, which control the recruitment and accumulation of B lymphocytes. The Liver X receptor (LXR)-dependent transcriptional network and the tetraspanin 6 (Tspan6) play key roles in this process by regulating the accumulation of oxysterol ligands in extracellular vesicles.
Article
Oncology
Pascale Mariani, Nouritza Torossian, Steven van Laere, Peter Vermeulen, Leanne de Koning, Sergio Roman-Roman, Olivier Lantz, Manuel Rodrigues, Marc-Henri Stern, Sophie Gardrat, Laetitia Lesage, Gabriel Champenois, Andre Nicolas, Alexandre Matet, Nathalie Cassoux, Vincent Servois, Emanuela Romano, Sophie Piperno-Neumann, Claire Lugassy, Raymond Barnhill
Summary: This study investigated the immune cell infiltrates in uveal melanoma liver metastases (UMLM) and found that CD68+ and CD163+ tumor-infiltrating macrophages, CD20+ peritumoral lymphocytes, and growth patterns were important prognostic factors.
BRITISH JOURNAL OF CANCER
(2023)
Article
Oncology
Xuemei Xie, Gaurav B. Chauhan, Ramakrishna Edupuganti, Takahiro Kogawa, Jihyun Park, Moises Tacam, Alex W. Tan, Mohd Mughees, Fnu Vidhu, Diane D. Liu, Juliana M. Taliaferro, Mary Kathryn Pitner, Luke S. Browning, Ju-Hyeon Lee, Yu Shen, Jian Wang, Naoto T. Ueno, Savitri Krishnamurthy, Gabriel N. Hortobagyi, Debu Tripathy, Steven J. Van Laere, Geoffrey Bartholomeusz, Kevin N. Dalby, Chandra Bartholomeusz
Summary: Triple-negative breast cancer (TNBC) is characterized by high relapse and metastasis rates, as well as a high proportion of cancer stem-like cells (CSC) with self-renewal and tumor initiation capacity. In this study, researchers found that MELK, a protein kinase known to promote CSC maintenance, is highly expressed in TNBC tumors and associated with worse overall survival and distant metastasis-free survival. Knockdown or inhibition of MELK reduces invasiveness, reverses epithelial-to-mesenchymal transition, and limits CSC self-renewal and maintenance in TNBC cells, leading to suppressed metastasis and improved overall survival in mouse models.
CANCER RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Charlotte Billiet, Wim Vingerhoed, Steven Van Laere, Ines Joye, Carole Mercier, Piet Dirix, Daan Nevens, Peter Vermeulen, Paul Meijnders, Dirk Verellen
Summary: CBCT guided and surface-guided spinal SABR showed very high precision in patient monitoring and treatment delivery, with lower intrafraction motion observed in patients treated with arms above their head and a body mask. Additionally, the acquisition of an extra CBCT was correlated with higher rotational motion during treatment, indicating the importance of monitoring during the procedure.
PHYSICS & IMAGING IN RADIATION ONCOLOGY
(2022)
Article
Oncology
Francois Bertucci, Laurys Boudin, Pascal Finetti, Christophe Van Berckelaer, Peter Van Dam, Luc Dirix, Patrice Viens, Anthony Goncalves, Naoto T. Ueno, Steven Van Laere, Daniel Birnbaum, Emilie Mamessier
Summary: The study revealed the heterogeneous immune profiles of inflammatory breast cancer, which showed similarity with melanoma TME. Inflammatory breast cancers displayed higher immune activity compared to non-inflammatory breast cancers, with most actionable immune genes being overexpressed in IBCs and associated with improved pathological response to chemotherapy.