Article
Hematology
Tae Ito, Yuko Suzuki, Hideto Sano, Naoki Honkura, Francis J. Castellino, Tetsumei Urano
Summary: The study demonstrates the existence of three distinct forms of high-molecular-weight complexes between tPA and PAI-1. Peptide sequence analysis revealed that these complexes consist of nonacylated-enzyme inhibitor complexes, with only a fraction of the population releasing free tPA under physiological conditions.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Health Care Sciences & Services
Hee Young Cho, Han Sung Park, Eun Hee Ahn, Eun Ju Ko, Hyeon Woo Park, Young Ran Kim, Ji Hyang Kim, Woo Sik Lee, Nam Keun Kim
Summary: The study revealed that PAI-1 rs1050955 and REN 10795 rs5707 alleles may decrease the risk of RPL, while the tPA rs4646972 genotype may increase the risk of RPL.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Genetics & Heredity
Rui Tang, Xiaohong Lyu, Hong Li, Jinlyu Sun
Summary: This study investigates the relationship between genetic susceptibility and the polymorphisms of seven genes in Chinese patients with allergy disorders. The results suggest that the polymorphism of PAI-1 rs1799762 may be associated with cough performance in Chinese allergic disease patients.
FRONTIERS IN GENETICS
(2023)
Article
Pharmacology & Pharmacy
Kirstin Meiners, Prisca Hamm, Marcus Gutmann, Jan Niedens, Agnieszka Nowak-Krol, Salvador Pane, Tessa Luehmann
Summary: The study developed three polyethylene glycol (PEG)ylated tPA bioconjugates based on the recombinant human tPA drug Alteplase using site-specific conjugation strategies. These bioconjugates showed improved proteolytic activity, slower inhibition kinetics by PAI-1, and increased hydrodynamic size.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Multidisciplinary Sciences
Sonia Selbonne, Celina Madjene, Benjamin Salmon, Yacine Boulaftali, Marie-Christine Bouton, Veronique Arocas
Summary: In this study, we found that in ischemic conditions, PN-1(-/-) mice showed increased capillary density, leukocyte recruitment, and elevated levels of IL-6 and MCP-1, promoting neoangiogenesis. This suggests that PN-1 limits neovascularization in pathological conditions.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, General & Internal
Shaowei Jiang, Yang Wang, Liang Chen, Honghua Mu, Connor Meaney, Yiwen Fan, Janesh Pillay, Hairong Wang, Jincheng Zhang, Shuming Pan, Chengjin Gao
Summary: This study investigates the associations among serum levels of PAI-1, PAI-1 gene polymorphisms, immunological indicators, and clinical outcomes in septic patients. The results suggest that genetic polymorphisms of PAI-1 can affect serum levels of PAI-1, which in turn may influence mortality by affecting neutrophil activity.
CHINESE MEDICAL JOURNAL
(2023)
Article
Medicine, Research & Experimental
Florence Boissiere-Michot, Caroline Mollevi, Volker Baecker, Evelyne Crapez, William Jacot
Summary: Urokinase plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (PAI-1) have been identified as prognostic and predictive biomarkers in breast cancer, but their clinical use is limited due to the need for fresh-frozen or fresh tumor samples for quantification. This study compared the expression levels of PLAU and SERPINE1 mRNA (corresponding to uPA and PAI-1 proteins) using in situ hybridization with immunometric assay in breast tumor samples. The results showed that in situ hybridization cannot replace the immunometric assay for quantification of uPA and PAI-1 expression.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Kaito Harada, Takashi Yahata, Makoto Onizuka, Abd Aziz Ibrahim, Eri Kikkawa, Toshio Miyata, Kiyoshi Ando
Summary: The study highlights the inhibitory role of plasminogen activator inhibitor type-1 (PAI-1) in hematopoietic regeneration in bone marrow microenvironments with abundant bone marrow adipocytes (BMAs), suggesting that blocking PAI-1 activity could be a novel therapeutic approach. PAI-1 inhibitor treatment significantly increases stem cell factor concentration in obese patients, contributing to improved hematopoietic regeneration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Angela Godinez, Rashi Rajput, Nitin Chitranshi, Veer Gupta, Devaraj Basavarajappa, Samridhi Sharma, Yuyi You, Kanishka Pushpitha, Kunal Dhiman, Mehdi Mirzaei, Stuart Graham, Vivek Gupta
Summary: Neuroserpin is a crucial protein involved in neuronal development and various diseases, with mutations affecting its function and leading to pathological conditions. Understanding the alterations of neuroserpin and its cellular signaling networks is essential for studying its detrimental consequences and potential therapeutic targeting.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Cardiac & Cardiovascular Systems
Steven J. Humphreys, Claire S. Whyte, Nicola J. Mutch
Summary: SERPINs are a class of inhibitors that trap and inhibit target enzymes through dynamic conformational changes. They play crucial inhibitory roles in regulating complex physiological enzymatic cascades and are associated with various health conditions. Recently, SERPINs have been implicated in immune response modulation and thromboinflammatory diseases. Understanding the physiological roles of SERPINs and their potential as biomarkers and therapeutic targets is essential in disease progression.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Endocrinology & Metabolism
Ziran Wang, Jintu Chen, Jingjing Song, Lingjun Kong, Xuanzhi Wang, Tengda Xu, Songbai Lin, Yaling Dou
Summary: This study used a robust detection method to determine the PAI-1 4G/5G genotype and investigated its association with diabetes mellitus, hypertension, and clinical characteristics. The results showed high concordance between the RT-PCR method and sequencing method for detecting PAI-1 4G/5G polymorphism. However, no association was found between the PAI-1 4G/5G polymorphism and genetic susceptibility to diabetes mellitus, hypertension, and diabetes mellitus combined with hypertension. Nevertheless, there were differences in clinical characteristics among individuals with different genotypes, which may provide reference for disease assessment and personalized treatment.
DIABETES METABOLIC SYNDROME AND OBESITY
(2023)
Review
Endocrinology & Metabolism
Yongzhuo Yu, Wenxuan Li, Lili Xu, Yangang Wang
Summary: Cardiovascular complications are a common cause of death in type 2 diabetes patients, and Plasminogen-activator Inhibitor 1 (PAI-1) plays a crucial role in their development and progression. Insulin resistance increases PAI-1 production, leading to an environment conducive to thrombosis and severe vascular disease. PAI-1 also exhibits circadian rhythm characteristics, which may explain the higher incidence of cardiovascular events in the morning. PAI-1 is a potential drug target, but currently, no PAI-1 inhibitors have been approved for clinical use. Chronotherapy, based on rhythm, may offer improved treatment for cardiovascular complications in diabetic patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Yukiko Asano, Takayuki Iwaki, Kazuo Umemura, Naohiro Kanayama, Hiroaki Itoh
Summary: The study showed that fibrin had no effect on mouse blastocysts, but significantly suppressed trophoblast growth in human blastocysts. However, trophoblasts in human blastocysts then switched to growth promotion through increased fibrinolysis with urokinase-type plasminogen activator (uPA) activity.
HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Claire S. Whyte, Hadj Ahmed. Mostefai, Kim M. Baeten, Andrew J. Lucking, David E. Newby, Nuala A. Booth, Nicola J. Mutch
Summary: The resolution of arterial thrombi relies on the endogenous fibrinolytic system. tPA primarily localizes to the thrombus head in a free and active form. Thrombi formed under high shear conditions are more resistant to fibrinolysis due to less tPA and increased PAI-1 concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kiyotaka Okada, Naoyuki Kawao, Daisho Nakai, Rei Wakabayashi, Yoshitaka Horiuchi, Katsumi Okumoto, Shinji Kurashimo, Yoshimasa Takafuji, Osamu Matsuo, Hiroshi Kaji
Summary: This study demonstrated that glucocorticoids decrease the number of macrophages at the injured site during early bone repair after femoral bone injury partly through PAI-1 and M-CSF in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Heesoo Uhm, Wooyoung Kang, Kook Sun Ha, Changwon Kang, Sungchul Hohng
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Yejin Lee, Byungho Lim, Seon Woo Lee, Woo Rin Lee, Yong-In Kim, Minhyeok Kim, Hyoungseok Ju, Mi Young Kim, Suk-Jo Kang, Ji-Joon Song, J. Eugene Lee, Changwon Kang
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2018)
Article
Oncology
Byungho Lim, Hee-Jin Kim, Haejeong Heo, Nanhyung Huh, Su-Jin Baek, Jong-Hwan Kim, Dong-Hyuck Bae, Eun-Hye Seo, Sang-Il Lee, Kyu-Sang Song, Seon-Young Kim, Yong Sung Kim, Mirang Kim
Article
Oncology
Seon Woo Lee, Do Youn Park, Mi-Young Kim, Changwon Kang
Article
Oncology
Eun-Hye Seo, Hee-Jin Kim, Jong-Hwan Kim, Byungho Lim, Jong-Lyul Park, Seon-Young Kim, Sang-Il Lee, Hyun-Yong Jeong, Kyu-Sang Song, Yong-Sung Kim
INTERNATIONAL JOURNAL OF CANCER
(2020)
Article
Multidisciplinary Sciences
Wooyoung Kang, Kook Sun Ha, Heesoo Uhm, Kyuhyong Park, Ja Yil Lee, Sungchul Hohng, Changwon Kang
NATURE COMMUNICATIONS
(2020)
Review
Pharmacology & Pharmacy
Jihyeob Mun, Gildon Choi, Byungho Lim
DRUG DISCOVERY TODAY
(2020)
Article
Biochemistry & Molecular Biology
Wooyoung Kang, Seungha Hwang, Jin Young Kang, Changwon Kang, Sungchul Hohng
Summary: DNA-binding proteins use sliding and hopping mechanisms for one-dimensional diffusion on DNA, while post-terminational RNA polymerases employ one-dimensional diffusion for recycling. The efficiency of reinitiation on promoters mainly depends on distance rather than direction, indicating efficient orientation change during hopping diffusion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Meeting Abstract
Hematology
Byungho Lim, Dabin Yoo, Kyung-jin Cho, Daeun Choi, Myoung Eun Jung, Doo Young Jung, Jin Soo Lee, Younghwa Chun, Areum Go, Ha Young Lee, Gildon Choi
Article
Multidisciplinary Sciences
Eunho Song, Heesoo Uhm, Palinda Ruvan Munasingha, Seungha Hwang, Yeon-Soo Seo, Jin Young Kang, Changwon Kang, Sungchul Hohng
Summary: This study provides evidence that Rho-mediated transcription termination can occur via three kinetically different routes, shedding light on the mechanism of transcription termination.
NATURE COMMUNICATIONS
(2022)
Letter
Oncology
Byungho Lim, Dabin Yoo, Younghwa Chun, Areum Go, Kyung-Jin Cho, Daeun Choi, Myoung Eun Jung, Ha Young Lee, Rebecca J. Boohaker, Jin Soo Lee, DooYoung Jung, Gildon Choi
BLOOD CANCER JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Eunho Song, Seungha Hwang, Palinda Ruvan Munasingha, Yeon-Soo Seo, Jin Young Kang, Changwon Kang, Sungchul Hohng
Summary: In this study on bacterial Rho factor-dependent terminators, it was found that the three Rho-dependent termination routes operate compatibly and their efficiency depends on the termination pauses. The most abundant route involves Rho catching up with a paused RNA polymerase, leading to simultaneous release of transcript RNA and template DNA. The fastest route results in RNA-only release and recycling of RNAP, while the slowest route facilitates simultaneous releases. The termination efficiency of stand-by Rho positively correlates with pause duration, contrary to previous speculation.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Gunhyoung Lim, Seungha Hwang, Kilwon Yu, Jin Young Kang, Changwon Kang, Sungchul Hohng
Summary: In this single-molecule study, researchers discovered that actively transcribing RNA polymerase can act as an efficient sensor for DNA double-strand breaks (DSBs), resulting in the formation of R-loops. The efficiency of R-loop formation is influenced by DNA end structures and can interfere with transcription and prevent subsequent rounds of transcription. These findings provide valuable insights into the initiation of DSB repair on transcription templates across different organisms.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Rheumatology
Taehyeung Kim, Sang-Cheol Bae, Changwon Kang
ARTHRITIS RESEARCH & THERAPY
(2020)