Article
Chemistry, Multidisciplinary
Yao Lu, Giulia Allegri, Jurriaan Huskens
Summary: Recruitment of receptors at membrane interfaces is essential in biological recognition and uptake processes. A model system based on the supported lipid bilayer (SLB) is demonstrated to mimic the recruitment process induced by weakly multivalent interactions. The recruitment of receptors induced by the binding of His(2)-functionalized vesicles on NiNTA-terminated SLBs is investigated to identify the ligand densities necessary for vesicle binding and receptor recruitment.
Review
Physiology
Cynthia N. Okoye, Pamela J. E. Rowling, Laura S. Itzhaki, Catherine Lindon
Summary: E3s are a structurally diverse group that target multiple substrates through specific protein-protein interactions. These interactions rely on short linear motifs in the disordered region of the substrate. Understanding the role of multiple degrons in E3-substrate interactions is crucial for targeted protein degradation. The Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ligase, serves as an important paradigm for E3-substrate docking.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Alexandra Tsouka, Kassandra Hoetzel, Marco Mende, Jasmin Heidepriem, Grigori Paris, Stephan Eickelmann, Peter H. Seeberger, Bernd Lepenies, Felix F. Loeffler
Summary: Multivalent ligand-protein interactions play a crucial role in enhancing the affinity and specificity of weak single glycan-protein interactions. Laser-based technology allows for in situ chemical synthesis of peptide scaffolds on functionalized glass slides, resulting in spatially defined multivalent glycopeptides. The study showed that surface functionalization, wettability, accessibility, and flexibility are essential factors in these interactions, and different types of surface functionalization and spacers may be required for optimal binding between lectins and glycans.
FRONTIERS IN CHEMISTRY
(2021)
Article
Clinical Neurology
Anneke Kuipers, Marta Balasko, Erika Petervari, Andreas Koller, Susanne M. Brunner, Gert N. Moll, Barbara Kofler
Summary: This study describes the development of a novel ligand, M89b, with specificity for GAL(2)R and increased stability by modifying a galanin-related peptide. M89b significantly reduced food intake in rats and showed promising potential for clinical application.
Article
Biochemistry & Molecular Biology
Kwabena Owusu Dankwah, Jonathon E. Mohl, Khodeza Begum, Ming-Ying Leung
Summary: This study computationally predicted the binding of a single ligand to GPCRs from different families and uncovered similar binding pockets that contribute to ligand interactions. These findings can be applied to improve protein function inference, drug repurposing, drug toxicity prediction, and the acceleration of new drug development.
Article
Medicine, Research & Experimental
Hui Liang, Ling Yun Tang, Hao Yang Ge, Ming Mei Chen, Shun Yuan Lu, Hong Xin Zhang, Chun Ling Shen, Yan Shen, Jian Fei, Gang Wang
Summary: The study identified ADGRL1 as a potential receptor for TAFA2, which directly binds to TAFA2 through its lectin-like domain. Overexpression of ADGRL1 induced apoptosis, but not ADGRL1 Delta Lec, and this apoptosis could be suppressed by recombinant TAFA2. These results suggest TAFA2 and its receptor ADGRL1 as potential therapeutic targets for neurological disorders.
Article
Biochemistry & Molecular Biology
Pamela J. E. Rowling, Ben L. Murton, Zhen Du, Laura S. Itzhaki
Summary: The interaction between beta-catenin and APC plays a crucial role in regulating the Wnt signaling pathway. APC acts as a scaffold, bringing together different components of the beta-catenin destruction complex to promote beta-catenin degradation. Mutations in APC can lead to elevated beta-catenin levels and abnormal Wnt signaling, which is associated with various types of cancer.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alessandro Palmioli, Luca Moretti, Carlo Alberto Vezzoni, Laura Legnani, Paola Sperandeo, Laura Baldini, Francesco Sansone, Cristina Airoldi, Alessandro Casnati
Summary: We synthesized a new class of multivalent glycoconjugates for the treatment of UTIs caused by UPEC. These compounds showed increased inhibitory potency against FimH-mediated adhesion processes compared to previous dendrimers, as demonstrated by yeast agglutination assay. Furthermore, the direct molecular interaction between the compounds and FimH protein was confirmed by on-cell NMR experiments.
BIOORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Lorenzo Di Rienzo, Luca De Flaviis, Giancarlo Ruocco, Viola Folli, Edoardo Milanetti
Summary: This study proposes a new computational approach based on Zernike polynomials for identifying binding sites of specific ligands on GPCRs. By analyzing the shape complementarity of local molecular surfaces, the method can distinguish binding pockets from randomly sampled regions on the receptor surface. The method achieves an Area Under ROC curve of 0.77 in experiments, demonstrating its ability for rapid and efficient screening of potential drugs.
JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN
(2022)
Article
Biophysics
Jayeon Song, Hansol Kim, Chang Yeol Lee, Junhyeok Yoon, Won Sang Yoo, Hyun Gyu Park
Summary: The novel method named Aptamer-assisted Protein-induced Fluorescence Enhancement (AptPIFE) successfully identifies the interaction between thyroid hormone and thyroid hormone receptor with high sensitivity and excellent specificity, proving practical and robust applicability by screening TR antagonists and quantifying thyroid hormone in clinical samples.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Materials Science, Multidisciplinary
Liang-Jun Pan, Jia-Wei Tu, Ling-Ling Yang, Zhi-Quan Tian, Zhi-Ling Zhang
Summary: This study demonstrates a novel strategy to prepare Ag2S quantum dots with high photoluminescence quantum yield (PLQY) through chloride-mediated growth and passivation. By partially exchanging ligands and passivating with chloride ions, the surface trap states in Ag2S QDs are effectively reduced, leading to a significant enhancement of photoluminescence intensity.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Chemistry, Physical
Timothy G. Mack, Juliana Spinelli, Mark P. Andrews, Patanjali Kambhampati
Summary: This study reports mode-specific resonance Raman enhancements of ligands bound to the surface of colloidal CdSe nanocrystals. The extent of enhancement is directly related to the molecular symmetry of the ligands, and further discussion is made regarding Franck-Condon and Herzberg-Teller contributions and associated selection rules. Resonance Raman can be used to distinguish between possible surface binding motifs of bidentate ligands under continuous wave excitation, demonstrating its usefulness as a characterization tool for adsorbed molecular species on semiconductor NC surfaces.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Pharmacology & Pharmacy
Sarina Gadgaard, Wijnand J. C. van der Velden, Sine P. Schiellerup, Jenna Elizabeth Hunt, Maria B. N. Gabe, Johanne Agerlin Windelov, Geke Aline Boer, Hannelouise Kissow, Cathrine Orskov, Jens J. Holst, Bolette Hartmann, Mette M. Rosenkilde
Summary: By studying the structure and functional characteristics of two radioligands, we found that subepithelial myofibroblasts are the main site of GLP-2 receptor expression.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hideo Ogiso, Ryoji Suno, Takuya Kobayashi, Masashi Kawami, Mikihisa Takano, Masaru Ogasawara
Summary: Molecular interaction analysis is crucial for studying biomolecular functions and developing new drugs. This study successfully used the SEC-MS method to detect the interaction between low-molecular-weight compounds and membrane proteins without the need for immobilization.
Article
Chemistry, Medicinal
Stefania Monteleone, Dmitri G. Fedorov, Andrea Townsend-Nicholson, Michelle Southey, Michael Bodkin, Alexander Heifetz
Summary: This article discusses the importance of protein-protein interactions and explores the use of the FMO method to identify molecular interactions at the protein-protein interface. The study shows that critical PPIs can be categorized into different types of interactions, providing important clues for further development of PPI modulators.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Chemistry, Physical
Michaela Roedel, Joerg Tessmar, Juergen Groll, Uwe Gbureck
Article
Polymer Science
Julia Liebscher, Joerg Tessmar, Juergen Groll
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2020)
Article
Engineering, Biomedical
I. A. D. Mancini, S. Schmidt, H. Brommer, B. Pouran, S. Schaefer, J. Tessmar, A. Mensinga, M. H. P. van Rijen, J. Groll, T. Blunk, R. Levato, J. Malda, P. R. van Weeren
Article
Polymer Science
Ali Nadernezhad, Leonard Forster, Francesca Netti, Lihi Adler-Abramovich, Joerg Tessmar, Juergen Groll
Article
Engineering, Biomedical
Julia Hauptstein, Thomas Boeck, Michael Bartolf-Kopp, Leonard Forster, Philipp Stahlhut, Ali Nadernezhad, Gina Blahetek, Alma Zernecke-Madsen, Rainer Detsch, Tomasz Jungst, Juergen Groll, Joerg Tessmar, Torsten Blunk
ADVANCED HEALTHCARE MATERIALS
(2020)
Article
Biochemistry & Molecular Biology
Stefanie Schmidt, Florencia Abinzano, Anneloes Mensinga, Joerg Tessmar, Juergen Groll, Jos Malda, Riccardo Levato, Torsten Blunk
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Rafael Schmid, Sonja K. Schmidt, Jonas Hazur, Rainer Detsch, Evelyn Maurer, Aldo R. Boccaccini, Julia Hauptstein, Jorg Tessmar, Torsten Blunk, Stefan Schrufer, Dirk W. Schubert, Raymund E. Horch, Anja K. Bosserhoff, Andreas Arkudas, Annika Kengelbach-Weigand
Article
Cell Biology
Hannes Horder, Mar Guaza Lasheras, Nadine Grummel, Ali Nadernezhad, Johannes Herbig, Sueleyman Erguen, Joerg Tessmar, Juergen Groll, Ben Fabry, Petra Bauer-Kreisel, Torsten Blunk
Summary: Biofabrication, including 3D printing technologies, is a powerful approach for designing disease models, such as in cancer research. A 3D-printed breast cancer model was developed to investigate cancer cell-adipocyte interaction, demonstrating adipogenic differentiation within printed spheroids. Co-culture of breast cancer cells with adipose tissue constructs showed reduced lipid content and ECM remodeling, highlighting the importance of cell-cell and cell-matrix interplay within tumor-stroma microenvironment.
Article
Biochemistry & Molecular Biology
Julia Hauptstein, Leonard Forster, Ali Nadernezhad, Juergen Groll, Joerg Tessmar, Torsten Blunk
Summary: In this study, a hyaluronic acid-based bioink was developed that allowed for the covalent tethering of TGF-beta 1, a growth factor important for cartilage regeneration. The functionality of the tethered TGF-beta 1 was successfully demonstrated in promoting chondrogenesis and maintaining its activity after 3D printing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Julia Hauptstein, Leonard Forster, Ali Nadernezhad, Hannes Horder, Philipp Stahlhut, Juergen Groll, Torsten Blunk, Joerg Tessmar
Summary: A new bioink platform using thiolated hyaluronic acid (HA-SH) enables effective 3D printing and chondrogenesis. By utilizing a dual-stage crosslinking approach with HA-SH of varying molecular weights, the bioink not only maintains printability but also significantly enhances the quality and stiffness of cultured constructs.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jessica S. Brand, Leonard Forster, Thomas Boeck, Philipp Stahlhut, Joerg Tessmar, Juergen Groll, Krystyna Albrecht
Summary: This article introduces a hydrogel prepared from pig gastric mucin (PGM), which has good biocompatibility and functional groups for chemical modifications. Compared to bovine submaxillary gland mucin, this hydrogel is more cost-efficient and easily accessible.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Chemistry, Medicinal
Raphael Mietzner, Ramona Pawlak, Ernst R. Tamm, Achim Goepferich, Rudolf Fuchshofer, Miriam Breunig
Summary: The loss of Schlemm's canal cell function may be the main cause of primary open-angle glaucoma, and Angpt-1 mimetic nanoparticles could potentially serve as a therapeutic option for restoring this function.
Article
Polymer Science
Emine Karakaya, Faina Bider, Andreas Frank, Joerg Tessmar, Lisa Schoebel, Leonard Forster, Stefan Schruefer, Hans-Werner Schmidt, Dirk Wolfram Schubert, Andreas Blaeser, Aldo R. Boccaccini, Rainer Detsch
Summary: In the context of bioprinting, targeted printing of cells at specific positions is a novel approach that offers new possibilities for life science engineering. However, achieving high cell survival rates on gel substrates remains a challenge. This study evaluates the use of alginate-dialdehyde-polyethylene glycol (ADA-PEG) inks for drop on demand (DoD) printing, analyzing their biochemical properties, printing process, cell distribution, and shear forces. The findings highlight the importance of PEG modifications and chain lengths in achieving stable hydrogels and biocompatibility, as well as the need to determine the optimal cell concentration for targeted cell printing.
Article
Biochemistry & Molecular Biology
Junwen Shan, Thomas Boeck, Thorsten Keller, Leonard Forster, Torsten Blunk, Juergen Groll, Joerg Tessmar
Summary: Hyaluronic acid-based hydrogels are commonly used as cell carriers in regenerative medicine, allowing for control over network formation through chemical modifications. This study explored a novel synthesis approach involving oxidation of HA to generate dialdehydes for further modifications or crosslinking via Schiff base chemistry.
Article
Engineering, Biomedical
Jonas Hazur, Rainer Detsch, Emine Karakaya, Joachim Kaschta, Joerg Tessmar, Dominik Schneidereit, Oliver Friedrich, Dirk W. Schubert, Aldo R. Boccaccini