Article
Chemistry, Applied
Lijuan Liang, Yimeng Sun, Chang Liu, Xianshun Zeng, Jianlong Zhao
Summary: A novel probe PTZ-2Cy has been developed for selective detection of ClO- through oxidation and cleavage of C??C bonds, showing low detection limit and excellent selectivity. The probe is capable of imaging HClO in L929 cells and holds promise for detecting hypochlorite ions in biological samples.
Article
Spectroscopy
Taorui Yang, Ke An, Shishen Zhang, Yifeng Han
Summary: In this work, a novel fluorescent probe BBy-T based on photoinduced electron transfer (PeT) was developed for the rapid, sensitive, and selective detection of hypochlorous acid (HClO) in an aqueous solution. The probe exhibited a distinct fluorescence turn-on response to HClO with a remarkable Stokes shift, immediate response, and low detection limit. Bioimaging results demonstrated that the probe could be used for real-time fluorescence imaging of living HeLa cells and zebrafish.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Spectroscopy
Yang Yang, Zhenli Guo, Jinting Ye, Chao-Ying Gao, Jinglin Liu, Limei Duan
Summary: Two sulfonate substituted rhodamine hydrophilic fluorescent probes RbS1 and RbS2 were designed and synthesized for specific detection of Fe3+. Both probes exhibited significant fluorescence enhancement response to Fe3+ and showed a good linear relationship with the concentration of Fe3+. RbS2 demonstrated better water solubility and fluorescence imaging ability in living fish compared to RbS1.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Veronika E. Reut, Stanislav O. Kozlov, Igor Kudryavtsev, Natalya A. Grudinina, Valeria A. Kostevich, Nikolay P. Gorbunov, Daria Grigorieva, Julia A. Kalvinkovskaya, Sergey B. Bushuk, Elena Yu Varfolomeeva, Natalia D. Fedorova, Irina Gorudko, Oleg M. Panasenko, Vadim B. Vasilyev, Alexey Sokolov
Summary: This paper introduces a fluorescent probe called Celestine Blue B (CB), which can be used for HOCl detection in living cells and for assaying the chlorinating activity of MPO. The probe shows remarkable selectivity and sensitivity, and has a rapid response to HOCl. It can also detect endogenous HOCl and reactive halogenated species using various techniques.
Article
Spectroscopy
Tiantian Jiang, Weiwei Bian, Jianfei Kan, Yanyan Sun, Ning Ding, Wenjing Li, Jin Zhou
Summary: A new probe RB-CR for detecting Cr3+ was constructed based on the conjugation between rhodamine B and phenylthiourea, showing selectively, sensitively and rapidly response to Cr3+ complexation. The fluorescence imaging study suggested its potential application as a surveillance probe in living cells with low cytotoxicity, providing feasibility for exploring the Cr3+ recognition process.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Organic
Xuefeng Wang, Zhenyuan Cheng, Juanjuan Wu, Fan Yang, Zhaozhi Zhang, Fang Tang, Wan Sun, Aixiang Ding
Summary: In this study, a PTZ fluorescent probe based on phenothiazine was successfully synthesized for specific detection of HClO. PTZ exhibited excellent selectivity, biocompatibility, rapid response, pH stability, and low limits of detection (5.2 nM). Furthermore, PTZ could detect both exogenous and endogenous HClO rapidly in living cells and zebrafish, showing its broad potential applications in life sciences.
Article
Chemistry, Analytical
Weiqing Xu, Farid Ahmed, Hai Xiong
Summary: A rhodamine hydrazide conjugating uridine moiety (RBH-U) was synthesized and developed as a fluorescence probe for selective detection of Fe3+ ions in an aqueous solution. The probe showed a 9-fold enhancement in fluorescence intensity upon the addition of Fe3+ ions, and exhibited remarkable specificity for Fe3+ with a detection limit of 0.34 mu M. Additionally, RBH-U can be used as a switch-off sensor for the recognition of Cu2+ ions and as a mitochondria-targeted fluorescent probe.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Yingying Li, Jie Zhang, Zengqiang Liang, Ran Yang, Lingbo Qu, Zhaohui Li, Yuanqiang Sun
Summary: In this paper, a new rhodamine derivate was synthesized and used as a phosgene probe with highly rapid, special and sensitive response towards phosgene. A fluorescent detection pen with gas-liquid reaction model was proposed for the first time to achieve in situ visualization of phosgene. The detection film could be formed in situ by simply writing on desired surface and respond toward phosgene gas within 30 s with an ultra-low detection limit (2 ppm) with the naked eye. Such a fluorescence pen detection mode provided an excellent platform for real-time detection of phosgene and other gas analytes.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Lidan Zhang, Chuang Wang, Yunhan Jiang, Huidong Li, Huizhen Wang, Haiyue Long, Pingxian Liu, Xiaoqiu Xu, Tao Yang
Summary: In this study, a new fluorescent probe IMQU-8 was developed for the selective and sensitive detection of Pd2+ under physiological conditions. The sensing mechanism of IMQU-8 was revealed, and its applicability for Pd2+ imaging and detoxification in living cells was demonstrated.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Qiuchen Liu, Chang Liu, Song He, Liancheng Zhao, Xianshun Zeng, Jin Zhou, Jin Gong
Summary: In this study, a novel fluorescent probe RHN was designed and synthesized for the sensing and imaging of HClO in mitochondria in living cells. RHN showed high selectivity, sensitivity, and rapid response, with detection limits as low as 22 nM. The cell imaging experiments demonstrated that RHN has the ability to detect and image HClO in mitochondria, which is significant for exploring the specific role of HClO in the immune system and diseases.
Article
Chemistry, Applied
Yu Min Wang, Jiali Li, Jian Hua Chen, Bo Liu, Yanyan Zeng, Ja-Yun Lim, Ya Ting Liu, Jun Feng Zhang, Xiang Hua Wu, Wen Xiu Ren
Summary: A dual-responsive fluorescent probe FDOCl-N-Na was synthesized for detecting two reactive oxygen species (ROSs) H2O2 and HClO. FDOCl-N-Na exhibited distinct responses towards H2O2 and HClO, showing green and red fluorescence respectively. It could simultaneously monitor the cellular changes of H2O2 and HClO with high selectivity and sensitivity over a wide pH range. Cell imaging experiments demonstrated its ability to differentiate cancer cells from normal cells based on the abnormal expression of H2O2 and HClO. Moreover, FDOCl-N-Na was also employed for tracking the fluctuation of H2O2 and HClO in Caenorhabditis elegans.
Article
Spectroscopy
Wen Chen, Xiaoping Lin, Xin Yin, Xuanyuan Wang, Dan Xie, Wenqing Tang, Cong Dai, Rongying Zeng, Mengqin Liu
Summary: In this study, an aggregation-induced emission (AIE) fluorescent probe has been designed for the highly sensitive and selective detection of Hg2+. The probe demonstrates outstanding detectivity and hypersensitivity for Hg2+ in aqueous solutions, and shows potential applications in environmental, food, and biological systems.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Wenting Zhang, Chunwei Yu, Mei Yang, Shaobai Wen, Jun Zhang
Summary: A small organic molecule P was synthesized and characterized as a dual-modal probe for Hg2+, exhibiting both fluorometric and colorimetric sensing capabilities. The probe showed high selectivity and sensitivity towards Hg2+ in ethanol-water solution, making it suitable for imaging Hg2+ in living cells with low cytotoxicity and good biocompatibility.
Article
Spectroscopy
Boxin Zhou, Beibei Wang, Mengqi Bai, Mingdong Dong, Xu Tang
Summary: Cysteine plays an important role in the human body, and abnormal concentration can lead to various diseases. Detecting cysteine in vivo with high selectivity and sensitivity is therefore significant. In this study, a small organic fluorescent probe ZHJ-X was designed and synthesized based on cyanobiphenyl, which specifically recognized cysteine. The probe ZHJ-X demonstrated specific selectivity, high sensitivity, short response time, good anti-interference ability, and a low detection limit of 3.8 x 10-6 M. It was successfully applied for visualizing cysteine in living cells and holds potential for cell imaging and detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Physical
Weiliang Deng, Qifan Wang, Shirui Xue, Peng Wang
Summary: In this study, a new dual-signals probe Rb-HMD with colorimetric and fluorescent was designed and synthesized. It exhibited highly selective recognition of trivalent ions (Fe3+, Al3+ and Cr3+) by fluorescence enhancement and colorimetric observation in the presence of other competing metal ions. The probe has good application prospects for high sensitive detection of Fe3+, Al3+ and Cr3+ in real samples and biological systems.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)