Journal
CANADIAN JOURNAL OF PLANT PATHOLOGY
Volume 36, Issue 2, Pages 141-150Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07060661.2014.899275
Keywords
16SrXXII phytoplasma; 'Candidatus Phytoplasma'; Cape St. Paul wilt disease; coconut; RFLP
Categories
Funding
- Cote d'Ivoire FIRCA (Fonds Interprofessionnel pour la Recherche et le Conseil Agricoles)/Cocotier CNRA - COC [588]
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Coconut palms from the Grand-Lahou region of Cote d'Ivoire exhibiting lethal yellowing-like symptoms and infected by a phytoplasma were further tested for the characterization of the phytoplasma pathogen based on the 16S rRNA gene analysis. Nested PCR with phytoplasma universal and lethal yellowing-specific primers resulted in amplification of products of expected sizes from seven out of 17 symptomatic trees tested, while non-infected symptomless plants yielded no DNA amplification. The 16S rDNA sequence of the phytoplasma detected from Grand-Lahou in coconut trees (KC999037) affected with the Cote d'Ivoire Lethal Yellowing disease showed a 99% sequence identity with that of the Ghanaian lethal yellowing phytoplasma (Cape St. Paul Wilt strain) of group 16SrXXII. The 16S rDNA sequence-based virtual and actual RFLP and phylogenetic analyses revealed that the phytoplasma associated with Cote d'Ivoire Lethal Yellowing in Grand Lahou is as a member of a new subgroup within group 16SrXXII that was designated as 16SrXXII-B. The present results support previous suspicions of disease spread to Cote d'Ivoire from neighbouring Ghana, posing a threat to the survival of the coconut trees in the Grand-Lahou region and for the Ivorian coconut industry.
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