Article
Environmental Sciences
Kavita Nair, Roda Al-Thani, Samir Jaoua
Summary: The study found that the altered genes cry10A and cyt1C in the Bacillus thuringiensis subsp. israelensis (Bti) strain QBT220, which led to the structural instability of plasmid pBtoxis, did not affect its higher insecticidal activity, indicating that these two genes are not essential for Bti's insecticidal activity. In addition, the increased synthesis of 6-endotoxins per cell in QBT220 makes it a potential strain for industrial production of bioinsecticides.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Multidisciplinary Sciences
Aurelie Babin, Jean-Luc Gatti, Marylene Poirie
Summary: Behavioural avoidance has benefits for animals facing environmental stressors, and this study shows that non-target organisms, including Drosophila species, also avoid Bt bioinsecticide. The research found that female Drosophila rapidly avoid laying eggs in the presence of Bt bioinsecticide, with potential benefits for the offspring and female's fitness.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Article
Environmental Sciences
Siyi Wu, Jianfeng Zhong, Qiqi Lei, Haoran Song, Shao-Fang Chen, Abdul Qadeer Wahla, Kalpana Bhatt, Shaohua Chen
Summary: The well-known bacterium Bacillus thuringiensis (Bt) has been extensively studied and developed as a biological insecticide. Recent research has discovered that Bt strains also have the potential to biodegrade environmental pollutants. Various xenobiotics, including inorganic pollutants, pesticides, organic tin, petroleum, polycyclic aromatic hydrocarbons, reactive dyes, and ibuprofen, have been reported to be degraded by Bt. This paper reviews the biodegradation ability of Bt for different types of pollutants, highlighting its potential as a powerful agent for environmental pollutant removal.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biology
Naureen Fatima, Abdul Rehman, DilAra Abbas Bukhari
Summary: The current investigation isolated and characterized toxic Bt. local isolates with 99% homology to Bti. prototoxin Bacillus thuringiensis, which contains the full length cry11 gene. The expression of partially purified Bt. protein under optimized conditions showed high toxicity against Aedes aegypti larvae, suggesting its potential as a biopesticide to control insect resistance.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Environmental Sciences
Brigitte Poulin, Gaetan Lefebvre, Samuel Hilaire, Laurence Despres
Summary: This study investigates the fate of Bacillus thuringiensis subsp. israelensis (Bti) in the environment. The research finds that spore density of Bti significantly increases in treated Mediterranean marshes, with an unexpected recrudescence in spring. It takes three to four years for spore density to return to normal levels after mosquito-control interruption. The importance of this study lies in demonstrating that the environmental effects of mosquito-control using Bti can surpass its short-term efficacy and should be addressed from a one-health perspective.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Chemistry, Multidisciplinary
Yujun Long, Haiyan Zhang, Guohui Liao, Minggui Chen, Xiangyan Chen, Lixin Qin, Caijun Chen, Zhuo Chen, Xiaomao Wu, Feng Zhu
Summary: Broadcasting emamectin benzoate (EB) granules into maize leaf whorls effectively controls fall armyworm (FAW). However, the distribution of EB in maize plants is unclear. In this study, EB granules were prepared and characterized using infrared spectrometry. The behavior of the granules in water and the release of EB were studied, and a method for determining EB in different parts of maize plants was established.
Article
Agronomy
Mengyuan Xia, Shahzad Munir, Yongmei Li, Ayesha Ahmed, Pengbo He, Yixin Wu, Xingyu Li, Ping Tang, Zaiqiang Wang, Pengfei He, Yuehu Wang, Yueqiu He
Summary: In this study, it was found that Bacillus subtilis YZ-1 isolated from naturally deceased Pieris rapae exhibited significant toxicity against the storage pest Tenebrio molitor. The researchers identified surfactins as the insecticidal compounds responsible for the activity.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Xuelian Li, Yanli Zhang, Yunda Zhan, Hongwei Tian, Bing Yan, Jun Cai
Summary: A new promoter P-rsi from Bacillus thuringiensis was discovered to allow abundant expression of vip3Aa, and by knocking out protease genes, the degradation of vip3Aa was greatly inhibited and its yield was significantly improved. These findings have reference value for the research and development of novel bioinsecticides.
PEST MANAGEMENT SCIENCE
(2023)
Article
Microbiology
Jeanne Romero-Severson, Thomas E. Moran, Donna G. Shrader, Francisco R. Fields, Susan Pandey-Joshi, Clayton L. Thomas, Emily C. Palmer, Joshua D. Shrout, Michael E. Pfrender, Shaun W. Lee
Summary: Bacteriocins are a diverse group of antimicrobial peptides found in various commensal and probiotic organisms. In this study, a novel strain of Bacillus safensis C3 was isolated from mung bean seeds, which exhibited antibacterial activity against human and plant pathogens. Genome sequencing revealed three biosynthetic systems in C3 that produce bacteriocin-based peptides, offering potential for therapeutic development and food safety applications.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Nay El-Khoury, Imene Bennaceur, Emilie Verplaetse, Stephane Aymerich, Didier Lereclus, Mireille Kallassy, Michel Gohar
Summary: During biofilm growth, dynamic exchanges occur between planktonic and sessile cells. The planktonic population grows until the biofilm forms, then rapidly decreases to zero, while recruited planktonic cells are found in specific areas with low sessile cell density within the biofilm, indicating spatial heterogeneity.
Article
Multidisciplinary Sciences
Jeffrey A. Fabrick, Chan C. Heu, Dannialle M. LeRoy, Ben A. DeGain, Alex J. Yelich, Gopalan C. Unnithan, Yidong Wu, Xianchun Li, Yves Carriere, Bruce E. Tabashnik
Summary: The study demonstrates that mutations introduced by gene editing in the ABCA2 gene can lead to resistance to the insecticidal protein Cry2Ab. The results suggest that HzABCA2 is a key candidate gene for monitoring Cry2Ab resistance in field populations of H. zea.
SCIENTIFIC REPORTS
(2022)
Review
Microbiology
Svetoslav Dimitrov Todorov, Iskra Vitanova Ivanova, Igor Popov, Richard Weeks, Michael Leonidas Chikindas
Summary: Representatives of the genus Bacillus are versatile microorganisms with widespread applications in traditional fermentation and modern biotechnology. They have numerous beneficial properties, serving as starter cultures for fermented foods, producers of enzymes, antibiotics, and bioactive peptides, and as probiotics for humans and animals. Despite their well-documented benefits, questions regarding their safety and spore formation in practical applications have been raised, prompting further exploration in this review.
CRITICAL REVIEWS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Hualin Liu, Jinshui Zheng, Dexin Bo, Yun Yu, Weixing Ye, Donghai Peng, Ming Sun
Summary: Bacillus thuringiensis (Bt) toxin genes have been widely utilized in genetically modified crops for pest control. The web-based tool BtToxin_Digger efficiently mines toxin genes from large-scale sequence data, enabling accurate prediction and downstream analysis and testing. This versatile tool can also be used to mine other targeting genes from genome and metagenome data with database replacement.
Article
Biotechnology & Applied Microbiology
Sungmin Jung, Chaerin Woo, Joanna Ivy Irorita Fugaban, Jorge Enrique Vazquez Bucheli, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: This study aimed to identify a bacteriocin-producing strain for controlling the food-borne pathogen Listeria monocytogenes. Five strains were isolated from kimchi with activity against the target bacterium, and ST05DL was selected for further study due to its specific inhibitory activity.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Review
Food Science & Technology
Laura M. Carroll, Rachel A. Cheng, Martin Wiedmann, Jasna Kovac
Summary: The Bacillus cereus group, also known as B. cereus sensu lato (s.l.), is a complex species containing multiple closely related lineages with varying pathogenic abilities. Taxonomic classification of these organisms is challenging due to conflicting changes proposed over the years. This review discusses the nomenclatural changes in the taxonomic space of B. cereus s.l., outlines contemporary taxonomic frameworks, and offers suggestions to avoid ambiguities in classification. Future directions and open problems in the taxonomic realm are also discussed.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Energy & Fuels
Marisol Perez-Rangel, Jose E. Barboza-Corona, German Buitron, Idania Valdez-Vazquez
BIOENERGY RESEARCH
(2020)
Article
Microbiology
America S. Gaona-Mendoza, Jose E. Barboza-Corona, Luz E. Casados-Vazquez
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2020)
Article
Energy & Fuels
Samuel Celaya-Herrera, Luz E. Casados-Vazquez, Idania Valdez-Vazquez, Francisco Barona-Gomez, Dennis K. Bideshi, Jose E. Barboza-Corona
Summary: Streptomyces sp. strain CC48 isolated from an oligotrophic environment exhibits high cellulolytic activity and potential for hydrolyzing maize and wheat substrates. Additionally, the study suggests that the Cuatro Cienegas Basin could be a potential source of efficient cellulolytic microorganisms.
BIOENERGY RESEARCH
(2021)
Review
Agriculture, Multidisciplinary
Ma de Lourdes Perez-Zavala, Juan C. Hernandez-Arzaba, Dennis K. Bideshi, Jose E. Barboza-Corona
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2020)
Article
Chemistry, Multidisciplinary
Daniel R. Flores, Luz E. Casados, Sandra F. Velasco, Ana C. Ramirez, Gilberto Velazquez
Article
Biotechnology & Applied Microbiology
America S. Gaona-Mendoza, Martha C. Bravo-Rivas, Jose E. Barboza-Corona, Julio A. Massange-Sanchez, Luz E. Casados-Vazquez
Summary: This study successfully produced thurincin H, ChiA74, and Cry proteins using Bacillus thuringiensis as a heterologous host, showing their antimicrobial and chitinolytic activity. Application of thurincin H in corn seeds also increased germination percentage and root length, suggesting potential for biocontrol and biostimulant product development.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Julio Armando Massange-Sanchez, Mario Alberto Nunez-Valle, Jose E. Barboza-Corona, Luz Edith Casados-Vazquez
Summary: The study found that the thnI gene does not provide immunity and is not required for the biosynthesis of thurincin H.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Food Science & Technology
Daniela Ruiz-De Anda, Luz E. Casados-Vazquez, Cesar Ozuna
Summary: The study confirmed the synergistic effect of combining thurincin H and power ultrasound technology on the inactivation of Listeria innocua and Escherichia coli in orange juice and milk, with a greater effect observed in orange juice samples.
Article
Biotechnology & Applied Microbiology
Tomas Ortiz-Rodriguez, Fernanda Mendoza-Acosta, Sheila A. Martinez-Zavala, Ruben Salcedo-Hernandez, Luz E. Casados-Vazquez, Dennis K. Bideshi, Jose E. Barboza-Corona
Summary: This study demonstrates that the bacteriocin thurincin H, produced by Bacillus thuringiensis, has antibacterial activity but lacks hemolytic properties. These findings are important for the potential use of thurincin H as an antibacterial peptide in medicine and food products.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biotechnology & Applied Microbiology
Marisol Perez-Rangel, Idania Valdez-Vazquez, Sheila A. Martinez-Zavala, Luz E. Casados-Vazquez, Dennis K. Bideshi, Jose E. Barboza-Corona
Summary: This study evaluated the inhibitory activity of compounds secreted by bacteria isolated from a hydrogen-producing bioreactor to understand their interactions in this community. The results showed that organic acids, non-proteinaceous compounds, and bacteriocin-like inhibitory substances (BLIS) produced by the bacteria had inhibitory effects, which could impact the dynamics of the microbial population during hydrogen generation.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Entomology
Maria Fernanda Vazquez-Ramirez, Jorge E. Ibarra, Luz Edith Casados-Vazquez, J. Eleazar Barboza-Corona, Ma Cristina Del Rincon-Castro
Summary: This study characterized the Bacillus thuringiensis strain LBIT-418 and identified its potential as a bioinsecticide. Genetic analysis revealed the presence of multiple insecticidal genes in the strain, and bioassays demonstrated its high toxicity against different pests. These findings highlight the suitability of this strain for the development of a bioinsecticide.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Veterinary Sciences
Jaquelina J. Guzman-Rodriguez, Fabiola Leon-Galvan, Jose E. Barboza-Corona, Mauricio Valencia-Posadas, Pedro D. Loeza-Lara, Monica Sanchez-Ceja, Alejandra Ochoa-Zarzosa, Joel E. Lopez-Meza, Abner J. Gutierrez-Chavez
JOURNAL OF VETERINARY SCIENCE
(2020)