Article
Agriculture, Multidisciplinary
Alexander A. Tkachenko, Maria S. Gancheva, Varvara E. Tvorogova, Lavrenty G. Danilov, Alexander V. Predeus, Irina E. Dodueva, Ludmila L. Lutova
Summary: Using RNA-seq, gene expression changes during early stages of crown gall development in radish were analyzed, revealing different pathways upregulated or downregulated in spontaneous tumorous radish compared to non-tumorous lines.
ANNALS OF APPLIED BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Haifa Ben Gharsa, Meriam Bouri, Amira Mougou Hamdane, Christina Schuster, Andreas Leclerque, Ali Rhouma
Summary: The study identifies a potential biological agent, B. velezensis MBY2, that can effectively suppress pathogenic Agrobacterium in plants, significantly reducing pathogen populations in maize and tomato plants. Through experiments, the strain demonstrates antagonistic effects in vitro and in vivo, effectively reducing gall development induced by the pathogen.
Article
Plant Sciences
Jie Bai, Tuo Yao, Xiaojun Lan, Yanshan Yang, Zhanjun Wang, Xinyou Wang
Summary: The Chinese dwarf cherry crown gall disease was identified in a nursery base in Ningxia Hui Autonomous Region, China, with a pathogen identified as A. tumefaciens/ biovar 1. The pathogenicity assay showed crown gall symptoms on tomato and sunflower stems after inoculation.
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Agronomy
Rong Xiao, Xiao-Peng Mu, Jian-Cheng Zhang, Shuai Zhang, Chun-Fen Zhang, Shu Deng, Jun-Jie Du, Peng-Fei Wang
Summary: This study aimed to isolate and identify the pathogens causing crown gall disease in Prunus humilis, as well as study their physiological, phylogenetic, opine type, and biological control characteristics. The major pathogenic strains were identified as Rhizobium rhizogenes and Agrobacterium tumefaciens. Some strains possessed genes related to the traditional opines. However, the current biological control strains showed limited efficacy against the pathogenic strains derived from P. humilis.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Serah Lim, Se Chul Chun, Jin-Won Kim
Summary: This study evaluated the resistance of 58 Korean cultivars and six foreign cultivars to crown gall disease. It found that 24 cultivars, including 22 Korean cultivars and 2 foreign cultivars, showed resistance to the disease. The study also discovered a strong correlation between the gall formation rate and the initial gall formation period.
PLANT PATHOLOGY JOURNAL
(2023)
Article
Microbiology
Lin Chen, Xinghong Wang, Yunpeng Liu
Summary: This study identified macrolactins as antagonistic compounds of Bacillus velezensis CLA178 against Agrobacterium tumefaciens C58, showing significant inhibitory effects on C58 cell division and transcription of genes involved in attachment to plants. The disruption of macrolactin production resulted in weaker biocontrol activity of C58, highlighting the importance of macrolactins in the biocontrol mechanisms of Bacillus velezensis CLA178.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jinmeng Shang, Xia Wang, Meng Zhang, Rongshui Wang, Chengjia Zhang, Haiyan Huang, Shuning Wang
Summary: This study reveals that the Rid-NC protein belonging to the Rid6 subfamily enhances the degradation of nicotine by Agrobacterium tumefaciens S33 through detoxification of a toxic imine intermediate, providing new insights into the metabolic process of N-heterocyclic aromatic compounds in microorganisms. The findings also show that proteins from the Rid family, including Rid6-subfamily proteins, have deaminase activity and can function in the metabolism of N-heterocyclic aromatic alkaloids, in addition to amino acid metabolism. The study contributes to a better understanding of the function of the Rid family of proteins.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Plant Sciences
J. E. Holmes, H. Sanghera, Zamir K. Punja
Summary: Crown gall, caused by Agrobacterium tumefaciens, was found for the first time on cannabis plants in licensed production facilities in British Columbia, Canada. The incidence of the disease was extremely low, estimated at 0.01% of total plants.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2023)
Review
Plant Sciences
Madhu Tiwari, Arun Kumar Mishra, Debasis Chakrabarty
Summary: Plants respond to Agrobacterium infection by mounting a three-layered immune response, which determines their susceptibility or resistance to the pathogen. Understanding the molecular dialogues between plants and Agrobacterium may provide important insights for the treatment of crown gall disease.
Article
Plant Sciences
Nan Xu, Qiyuan Yang, Xiaojing Yang, Mingqi Wang, Minliang Guo
Summary: This study utilized a genome-scale metabolic model to investigate the metabolic characteristics of Agrobacterium tumefaciens and found differences in metabolic activity in different ecological niches. By integrating transcriptome data under tumor conditions, significant changes in metabolic pathways were identified.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Fiqih Ramadhan, Yuzer Alfiko, Sigit Purwantomo, Andhika Faisal Mubarok, Widyah Budinarta, Antonius Suwanto, Sri Budiarti
Summary: Research has found that the overgrowth of Agrobacterium tumefaciens after plant transformation can be controlled using bacteriophage. Two lytic bacteriophages, Phi K2 and Phi K4, were effective in reducing the overgrowth of A. tumefaciens. Both phages showed different characteristics and growth kinetics, but had the same optimum temperature and pH. The combination of both bacteriophages with antibiotics completely eradicated A. tumefaciens.
Article
Microbiology
Si-Chong Wang, Ai-Ping Chen, Shu-Jen Chou, Chih-Horng Kuo, Erh-Min Lai
Summary: The T6SS is used by proteobacteria to secrete effector proteins and influence bacterial competition or pathogenesis. In this study, the researchers demonstrated that the T6SS affects disease occurrence and the composition of the microbial community in crown galls.
Review
Biochemistry & Molecular Biology
Dhanashree Vijayra Bomle, Asha Kiran, Jeevitha Kodihalli Kumar, Lavanya Senapathyhalli Nagaraj, Chamanahalli Kyathegowda Pradeep, Mohammad Azam Ansari, Saad Alghamdi, Ahmed Kabrah, Hamza Assaggaf, Anas S. Dablool, Mahadevamurthy Murali, Kestur Nagaraj Amruthesh, Arakere Chunchegowda Udayashankar, Siddapura Ramachandrappa Niranjana
Summary: Soil salinity stress is a major obstacle to food production worldwide, with strategies such as plant breeding and genetic engineering improving plant resistance to salinity stress to meet future food demand. The use of PGPR with ACC deaminase activity has shown promise in mitigating the impact of saline conditions and improving seed germination and seedling vigor. The variation of antioxidants and osmolytes in ACC deaminase-producing PGPR treated plants is also examined.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Kuang-Teng Wang, Ming-Chang Hong, Yu-Sheng Wu, Tsung-Meng Wu
Summary: Duckweed is one of the smallest flowering plants in the world and is used for livestock as biofactors and feedstuff additives due to its high reproduction rate and biomass. This study successfully established a micropropagation technique and Agrobacterium-mediated transformation in duckweed.
Article
Plant Sciences
Huimin Ren, Yan Xu, Xiaohong Zhao, Yan Zhang, Jamshaid Hussain, Fuqiang Cui, Guoning Qi, Shenkui Liu
Summary: Casuarina equisetifolia is widely used for agroforestry plantations for various purposes. This study established an efficient regeneration system using stem segment explants, optimizing callus induction and regeneration processes. Genetic transformation protocol using Agrobacterium tumefaciens was also optimized, leading to improved transformation efficiency to obtain transgenic plants in about 4 months. The findings will pave the way for further genetic manipulation and functional genomics of C. equisetifolia.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Briallen Lobb, Rhiannon Hodgson, Michael D. J. Lynch, Michael J. Mansfield, Jiujun Cheng, Trevor C. Charles, Josh D. Neufeld, Paul M. Craig, Andrew C. Doxey
Editorial Material
Biochemistry & Molecular Biology
M. J. Ryan, M. Schloter, G. Berg, T. Kostic, L. L. Kinkel, K. Eversole, J. A. Macklin, B. Schelkle, M. Kazou, I. Sarand, B. K. Singh, D. Fischer, E. Maguin, I. Ferrocino, N. Lima, R. S. McClure, T. C. Charles, R. S. C. de Souza, G. S. Kiran, H. L. Krug, J. Taffner, H. Roume, J. Selvin, D. Smith, D. Rybakova, A. Sessitsch
Summary: The microbiome research field is rapidly advancing and requires a well-established biobanking infrastructure for preserving microbiome samples and their functional potential. There is an urgent need for assessing how biobanks can support research in this rapidly evolving field.
TRENDS IN MICROBIOLOGY
(2021)
Letter
Microbiology
Gabriele Berg, Daria Rybakova, Doreen Fischer, Tomislav Cernava, Marie-Christine Champomier Verges, Trevor Charles, Xiaoyulong Chen, Luca Cocolin, Kellye Eversole, Gema Herrero Corral, Maria Kazou, Linda Kinkel, Lene Lange, Nelson Lima, Alexander Loy, James A. Macklin, Emmanuelle Maguin, Tim Mauchline, Ryan McClure, Birgit Mitter, Matthew Ryan, Inga Sarand, Hauke Smidt, Bettina Schelkle, Hugo Roume, G. Seghal Kiran, Joseph Selvin, Rafael Soares Correa de Souza, Leo van Overbeek, Brajesh K. Singh, Michael Wagner, Aaron Walsh, Angela Sessitsch, Michael Schloter
Correction
Biochemistry & Molecular Biology
M. J. Ryan, M. Schloter, G. Berg, T. Kostic, L. L. Kinkel, K. Eversole, J. A. Macklin, B. Schelkle, M. Kazou, I. Sarand, B. K. Singh, D. Fischer, E. Maguin, I. Ferrocino, N. Lima, R. S. McClure, T. C. Charles, R. S. C. de Souza, G. S. Kiran, H. L. Krug, J. Taffner, H. Roume, J. Selvin, D. Smith, D. Rybakova, A. Sessitsch
TRENDS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Gem Thomas-Barry, Chaney C. G. St Martin, Michael D. J. Lynch, Adesh Ramsubhag, Judy Rouse-Miller, Trevor C. Charles
Summary: This study investigated the changes in rhizobacteriome community structure in hyper-seasonal edaphic savannas, showing that soil and season were key determinants influencing the community structure. Plant biomass, root traits, and rhizobacterial community compositions were found to be influenced by seasonal changes and savanna-specific drivers.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Stuart J. Smyth, Trevor C. Charles
Summary: Digital sequence information (DSI), defined as nonphysical genetic resources like genetic sequence data, lacks a clear definition and is not consistent among countries. Restricting the free sharing of DSI may conflict with core principles in fields like microbiology and molecular genetics, potentially impacting international research collaborations.
TRENDS IN BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Tam T. Tran, Trevor C. Charles
Summary: Promoter engineering can enhance bio-product production, but the activity of a promoter may vary in different gene contexts. Inserted genes can affect promoter activity, leading to changes at the transcriptional level, which influence downstream applications.
Article
Biodiversity Conservation
Javiera Soto, Trevor C. Charles, Michael D. J. Lynch, Giovanni Larama, Hector Herrera, Cesar Arriagada
Summary: A novel strain of Brevundimonas sp. capable of growing under high doses of arsenic was isolated from metal(loid) contaminated soil in central Chile. Real-time PCR analysis revealed down-regulation of the arsB gene involved in arsenic efflux, while arsR, arsH, and ACR3 genes showed no significant differences in expression levels with the addition of arsenic.
Editorial Material
Microbiology
Vanessa A. Varaljay, Trevor C. Charles, Rolf Daniel
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xueya Zhang, Shuang Liang, Qingnan Wu, Trevor C. Charles, Rui He, Jiakai Wu, Yuhui Zhao, Zhiyi Zhao, Hezhong Wang
Summary: This study investigated the mode of action of nanochitin whisker (NC) against Fusarium pseudograminearum (Fpg), an important pathogenic fungus causing wheat crown rot disease. The results showed that NC significantly reduced hyphal growth and spore germination rate of Fpg. It exerted its effects by disrupting cell wall structure, altering membrane integrity, decreasing enzyme activity related to energy-supply, and reducing mycotoxin production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Environmental Sciences
Aleksander Cholewinski, Eugenia Dadzie, Cassandra Sherlock, William A. Anderson, Trevor C. Charles, Komal Habib, Steven B. Young, Boxin Zhao
Summary: The resilience and low cost of plastics have led to their widespread use, but also contribute to their abundance and persistence as waste. Plastic pollution is a major concern for global ecosystems and their health. Material flow analysis, previously used in other industries, has been applied to plastics to understand how waste enters the environment. However, research on degradation strategies for microplastics has been limited and material flow analysis lacks data. The variety of plastics, their additives, and contaminants present significant challenges in degrading microplastic particles.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Rashmi Chandra, Aranksha Thakor, Tizazu H. Mekonnen, Trevor C. Charles, Hyung-Sool Lee
Summary: The production of polyhydroxyalkanoates (PHAs) with high carboxylate concentration accumulated from solid-state fermentation (SSF) of food waste (FW) was tested using Pseudomonas putida strain KT2440. The mixed-culture SSF of FW supplied in a high carboxylate concentration led to a high PHA production of 0.56 g PHA/g CDM under nutrients control. The dominant PHA building block produced was 3-hydroxybutyrate, followed by 3-hydroxy-2-methylvalerate and 3-hydroxyhexanoate, indicating that acetate, butyrate, and propionate were the main precursors to PHA.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Microbiology
Meghan Donovan, Calvin S. Mackey, Michael D. J. Lynch, Grayson N. Platt, Amber N. Brown, Brian K. Washburn, Darryl J. Trickey, J. Thomas Curtis, Yan Liu, Trevor C. Charles, Zuoxin Wang, Kathryn M. Jones
Summary: Research on the role of gut microbiota in behavior has advanced significantly. The probiotic L. reuteri can affect social and stress-related behaviors, but the mechanisms behind this are still unclear. Using prairie voles, a socially-monogamous species, the study found that treatment with live L. reuteri led to decreased social affiliation compared to heat-killed L. reuteri in females but not males. There were also differences in neurochemical markers, gut microbiome composition, and behaviors between the sexes and treatments.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Angela Sessitsch, Steve Wakelin, Michael Schloter, Emmanuelle Maguin, Tomislav Cernava, Marie-Christine Champomier-Verges, Trevor C. Charles, Paul D. Cotter, Ilario Ferrocino, Aicha Kriaa, Pedro Lebre, Don Cowan, Lene Lange, Seghal Kiran, Lidia Markiewicz, Annelein Meisner, Marta Olivares, Inga Sarand, Bettina Schelkle, Joseph Selvin, Hauke Smidt, Leo van Overbeek, Gabriele Berg, Luca Cocolin, Yolanda Sanz, Wilson Lemos Fernandes, S. J. Liu, Matthew Ryan, Brajesh Singh, Tanja Kostic
Summary: Microbiomes play crucial roles in ecosystem functioning, supporting planetary health through nutrient cycling, climate regulation, and water filtration. They are closely associated with complex multicellular organisms and contribute to the health of their hosts. However, there is still limited understanding of microbiome transfer and connectivity.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Review
Biotechnology & Applied Microbiology
Nicole LeBlanc, Trevor C. Charles
Summary: Bacterial cells are commonly used for the production of value-added products. However, their own metabolism, genetic instability, and the toxicity of the product often hinder efficient production. Genome reductions have been performed to create strains that can serve as chassis for downstream applications. Despite improvements in cellular characteristics, the construction of these cells efficiently and rapidly remains a major challenge.
FRONTIERS IN GENOME EDITING
(2022)
