Journal
CANADIAN JOURNAL OF MICROBIOLOGY
Volume 56, Issue 2, Pages 162-167Publisher
NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS
DOI: 10.1139/W09-125
Keywords
bacteriocin; thuricin S; Bacillus thuringiensis; pore-forming activity
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Funding
- Agence universitaire de la Francophonie (AUF)
- United Nations Educational, Scientific, and Culture Organization (UNESCO)
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Different methods were used to elucidate the mode of action of thuricin S. a new class IId bacteriocin produced by Bacillus thuringiensis subsp. entomocidus HD198. According to cell viability tests, thuricin S was shown to exert a bactericidal effect on the sensitive cells of Bacillus thuringiensis subsp. darmastadiensis 10T. The use of the fluorescent probe 3,3'-dipropylthiadicarbocyanine iodide as an indicator proved that thuricin S interacts with the cytoplasmic membrane to dissipate the transmembrane potential. It was also demonstrated that thuricin S acts as a pore-forming bacteriocin, since it allows the nonpermeable stain propidium iodide to enter the cells. The loss of membrane integrity and the morphological changes in sensitive cells were visualized by scanning electron microscopy.
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