Article
Engineering, Environmental
Siwei Gu, Emma Mary Boase, Christopher Q. Lan
Summary: This study found that manipulating the concentration of dissolved inorganic carbon (DIC) in the cultivation medium can significantly enhance the Pb(II) adsorption capacity of green alga by increasing the charges on the cell surface. The presence of functional groups containing C=O, such as carboxyl, plays a major role in this adsorption enhancement process.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Analytical
Cynthia M. Galicia-Medina, Matias Vazquez-Pinon, Gibran S. Aleman-Nava, Roberto C. Gallo-Villanueva, Sergio O. Martinez-Chapa, Marc J. Madou, Sergio Camacho-Leon, Jonathan S. Garcia-Perez, Diego A. Esquivel-Hernandez, Roberto Parra-Saldivar, Victor H. Perez-Gonzalez
Summary: The study conducted a screening of Neochloris oleoabundans microalgae with different cytoplasmic lipid content using DEP technology, showing significant differences in dielectrophoretic behavior between N+ and N- cells at low frequencies. Additionally, finite element analysis was used to determine trapping zones across electrode gaps.
Article
Biotechnology & Applied Microbiology
Denisse Tatiana Molina-Aulestia, Carlos Ricardo Soccol, Antonio Irineudo Magalhaes Junior, Oranys Isaac Gonzalez-Marin, Caroline Gusmao de Souza, Adriane Bianchi Pedroni Medeiros, Eduardo Klein, Julio Cesar de Carvalho
Summary: Microalgae are emerging as sustainable sources for bioproducts and bioenergy, but face challenges with biological contamination. This study evaluated the toxicity of natural terpenes towards Neochloris oleoabundans, showing low toxicity levels with selected compounds, suggesting their potential as environmentally friendly alternatives for managing microalgal cultures.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Paeka Klaitong, Akaraphol Watcharawipas, Sirirat Fa-aroonsawat, Wipa Chungjatupornchai
Summary: This study investigated the regulation of diacylglycerol acyltransferase 2 gene (NeoDGAT2) in microalgae by analyzing the regulatory sequence RDG, revealing the potential role of MYB transcription factor in controlling TAG biosynthesis. The interaction between MYB transcription factor and MYB-bs may play a crucial role in regulating -N induced expression of NeoDGAT2 and affecting TAG accumulation, making MYB transcription factors a potential target for engineering to increase TAG content for economic viability.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Engineering, Chemical
Siwei Gu, Yifan Su, Christopher Q. Lan
Summary: Green alga Neochloris oleoabundans cultivated in medium of varied phosphate concentration showed effective biosorption capacity for Cu(II) removal from aqueous solutions. The highest biosorption capacity of 180.19 mg-Cu/g-biomass was achieved with biomass grown in medium containing 300 mg/L K2HPO4. The Cu(II) biosorption was fast with equilibrium established within 10 min of contact time, and Cu(II)-induced cell aggregation could facilitate biomass recovery.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2022)
Article
Plant Sciences
Costanza Baldisserotto, Valentina Gentili, Roberta Rizzo, Chiara Di Donna, Luna Ardondi, Annalisa Maietti, Simonetta Pancaldi
Summary: Microalgae, specifically Neochloris oleoabundans, cultivated under autotrophic and mixotrophic conditions, were found to produce whole aqueous extracts with antiviral properties against HCoV-229E. The extracts contained bioactive compounds such as lipids, exo-polysaccharides, phenolics, proteins, and pigments. The study suggests that the inhibitory effect of the extracts is mainly attributed to the combination of proteins, pigments, and phenolics in autotrophic cultures, and proteins, acidic exo-polysaccharides, and lipids in mixotrophic cultures.
Article
Biotechnology & Applied Microbiology
Denisse Tatiana Molina-Aulestia, Julio Cesar de Carvalho, Gilberto Vinicius de Melo Pereira, Alexander da Silva Vale, Dao Pedro de Carvalho, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: Biological contamination is a major problem in microalgae production, affecting quality and productivity. Using terpenes for contamination control in microalgae cultures is a potential solution.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Magda Vargas-Perez, Azucena Gonzalez-Horta, Hiram Mendoza-Hernandez, Myriam Elias-Santos, Karim Acuna-Askar, Luis Jesus Galan-Wong, Hugo Alberto Luna-Olvera
Summary: This study tested a new method using melittin peptide to induce cell disruption in N. oleoabundans, resulting in increased lipid recovery. Scanning electron microscopy and confocal images showed that melittin caused extensive cell damage by degrading the cell wall and releasing intracellular material.
BIOTECHNOLOGY LETTERS
(2023)
Article
Polymer Science
Ismail A. Walbi, Mohammad Zaki Ahmad, Javed Ahmad, Mohammed S. Algahtani, Amer S. Alali, Samar A. Alsudir, Alhassan H. Aodah, Hassan A. Albarqi
Summary: In this study, Curcumin-loaded lecithin-chitosan nanoparticles were developed and optimized using a statistical design. The optimized formulation showed small particle size, good stability, high entrapment efficiency, and controlled release, making it suitable for high loading and controlled release of lipophilic drugs.
Article
Medicine, Research & Experimental
Mukta Agrawal, Shailendra Saraf, Madhulika Pradhan, Ravish J. Patel, Gautam Singhvi, Ajazuddin, Amit Alexander
Summary: This study successfully optimized the formulation of curcumin-loaded nanolipid carrier with good stability and higher drug entrapment, which is in an acceptable size range for brain delivery.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Ahmed Saud Abdulhameed, Ali H. Jawad, Elmira Kashi, Khairul Adzfa Radzun, Zeid A. ALOthman, Lee D. Wilson
Summary: In this study, microalgae (MAG) was used as an environmentally friendly and efficient adsorbent for the removal of crystal violet (CV) and methylene blue (MB) dyes from aqueous solutions. The adsorption process was optimized using a Box-Behnken design (BBD), and the kinetics and isotherm analyses revealed that the adsorption followed the pseudo-second-order and Langmuir models. MAG exhibited a maximum adsorption capacity of 243.0 mg/g for CV and 297.1 mg/g for MB. The thermodynamic parameters indicated that the adsorption of CV and MB dyes onto MAG was spontaneous and endothermic. The adsorption mechanism involved electrostatic forces, n-?? stacking, and H-bonding. Overall, MAG shows promise as an effective adsorbent for removing dyestuffs from industrial effluent.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Pharmacology & Pharmacy
Joao Vieira, Jessica Castelo, Marta Martins, Nuno Saraiva, Catarina Rosado, Catarina Pereira-Leite
Summary: Transfersomes are a nanotechnology-based approach to enhance the delivery of bioactive compounds to the skin. However, their properties still need improvement for better application in the pharmaceutical industry and development of topical medicines. This study optimized transfersomes for cutaneous applications by incorporating mixed edge activators with opposing hydrophilic-lipophilic balance (HLB) using a Box-Behnken factorial design (BBD) strategy.
Article
Engineering, Environmental
Shweta Tripathi, Tapasranjan Behera, Krishna Mohan Poluri
Summary: This study investigates the efficiency of microalgae-based remediation in removing cadmium from water bodies. The results demonstrate that Coccomyxa sp. IITRSTKM4 can effectively remove cadmium and survive cadmium stress by regulating its survival mechanisms. Furthermore, the study also highlights the potential of Coccomyxa sp. in producing biofuels.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Pharmacology & Pharmacy
Fahad Mohammed AlMulhim, Anroop B. Nair, Bandar Aldhubiab, Hiral Shah, Jigar Shah, Vivek Mewada, Nagaraja Sreeharsha, Shery Jacob
Summary: This study investigates the feasibility of developing a buccal film impregnated with paliperidone-loaded nanostructured lipid carriers (NLCs) and demonstrates its potential as an alternative therapy for the treatment of schizophrenia, with improved bioavailability. The optimized NLC dispersions showed desired characteristics and the developed film exhibited excellent properties. The pharmacokinetics profile in rabbits supports the efficacy of the buccal therapy, showing higher AUC(0-12) and relative bioavailability compared to the control.
Article
Biotechnology & Applied Microbiology
Hongying Zhou, Zitong Xu, Liyuan Zhou, Zisheng Zhang, Ju Wang, Christopher Q. Lan
Summary: A horizontal thin-layer algal reactor was used to culture oil-rich green alga, Neochloris oleoabundans, with a high cell density. CO2-enriched air and a hollow fiber membrane sparger were used for aeration, allowing for pH control within a narrow range. The optimal nitrate concentration was 345 mg-N/L, resulting in high cell density and biomass concentrations. Intermittent mixing was found to save energy and reduce water evaporation, while also producing similar cell growth profile to continuous mixing. The findings suggest that it is technically feasible to cultivate photoautotrophic microalgae with high density using intermittent mixing and no culture circulation, leading to the development of cost-effective algal farming systems.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Johnson E. Efome, Dipak Rana, Takeshi Matsuura, Christopher Q. Lan
ACS APPLIED MATERIALS & INTERFACES
(2018)
Article
Nanoscience & Nanotechnology
Fan Yang, Johnson E. Efome, Dipak Rana, Takeshi Matsuura, Christopher Lan
ACS APPLIED MATERIALS & INTERFACES
(2018)
Review
Biotechnology & Applied Microbiology
Chinchin Wang, Christopher Q. Lan
BIOTECHNOLOGY ADVANCES
(2018)
Article
Engineering, Environmental
Johnson E. Efome, Dipak Rana, Takeshi Matsuura, Christopher Q. Lan
CHEMICAL ENGINEERING JOURNAL
(2018)
Article
Biophysics
Zeqing Bao, Christopher Q. Lan
COLLOIDS AND SURFACES B-BIOINTERFACES
(2018)
Article
Environmental Sciences
Zeqing Bao, Jiahui Cao, Guangbo Kang, Christopher Q. Lan
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2019)
Article
Engineering, Chemical
Hooman Chamani, Takeshi Matsuura, Dipak Rana, Christopher Q. Lan
JOURNAL OF MEMBRANE SCIENCE
(2019)
Article
Engineering, Chemical
Zhelun Li, Dipak Rana, Takeshi Matsuura, Christopher Q. Lan
SEPARATION AND PURIFICATION TECHNOLOGY
(2019)
Article
Environmental Sciences
Johnson E. Efome, Dipak Rana, Takeshi Matsuura, Christopher Q. Lan
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Engineering, Chemical
Rufan Zhou, Dipak Rana, Takeshi Matsuura, Christopher Q. Lan
SEPARATION AND PURIFICATION TECHNOLOGY
(2019)
Review
Green & Sustainable Science & Technology
Yifan Yang, Gary Cui, Christopher Q. Lan
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2019)
Review
Biophysics
Zeqing Bao, Christopher Q. Lan
COLLOIDS AND SURFACES B-BIOINTERFACES
(2019)
Article
Engineering, Chemical
Hooman Chamani, Pelin Yazgan-Birgi, Takeshi Matsuura, Dipak Rana, Mohamed I. Hassan Ali, Hassan A. Arafat, Christopher Q. Lan
Review
Environmental Sciences
Licheng Peng, Dongdong Fu, Huaiyuan Qi, Christopher Q. Lan, Huamei Yu, Chengjun Ge
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Endocrinology & Metabolism
Jessica Lee, Zachary Pappalardo, Deeksha Gambhir Chopra, Thomas G. Hennings, Ian Vaughn, Christopher Lan, Justin J. Choe, Kenny Ang, Steven Chen, Michelle Arkin, Michael T. McManus, Michael S. German, Gregory M. Ku
