Article
Biochemistry & Molecular Biology
Francesca Manuela Johnson de Sousa Brito, Andrew Butcher, Addolorata Pisconti, Blandine Poulet, Amanda Prior, Gemma Charlesworth, Catherine Sperinck, Michele Scotto di Mase, Ke Liu, George Bou-Gharios, Robert Jurgen van 't Hof, Anna Daroszewska
Summary: Syndecan-3 promotes new bone formation in osteoblasts by enhancing WNT signaling, and its deficiency in mice results in an early osteoporosis-like phenotype due to delayed osteoblast maturation and impaired function. Overexpressing Sdc3 in osteoblasts rescues the low bone volume phenotype and increases bone mass, making SDC3 a potential target for novel bone anabolic drug development.
Article
Biochemistry & Molecular Biology
Meng Chen, Liying Shan, Ying Gan, Lijie Tian, Jie Zhou, Endong Zhu, Hairui Yuan, Xiaoxia Li, Baoli Wang
Summary: MTSS1 plays a role in osteoblast differentiation and bone homeostasis by regulating Src-Wnt/beta-catenin signaling.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell & Tissue Engineering
Xiaowen Wu, Xiaochen Wang, Liying Shan, Jie Zhou, Xin Zhang, Endong Zhu, Hairui Yuan, Baoli Wang
Summary: The loss of NFIX gene affects endochondral ossification and mineralization in bone, with NFIX inducing osteoblast differentiation and impairing adipocyte formation by regulating HMGA1 expression. HMGA1 plays a role in osteoblast commitment and mediates NFIX function by activating canonical Wnt signaling pathways.
Article
Endocrinology & Metabolism
Bin-Feng Cheng, Xiao Feng, Yao-Xin Gao, Shao-Qin Jian, Shi-Rao Liu, Mian Wang, Yun-Fei Xie, Lei Wang, Zhi-Wei Feng, Hai-Jie Yang
Summary: NCAM regulates osteoblast differentiation through activation of the Wnt/β-catenin and PI3K-Akt signaling pathways, with its silencing inhibiting osteoblastic differentiation in various cell lines.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
David Buechel, Nami Sugiyama, Natalia Rubinstein, Meera Saxena, Ravi K. R. Kalathur, Fabiana Luond, Vida Vafaizadeh, Tomas Valenta, George Hausmann, Claudio Cantu, Konrad Basler, Gerhard Christofori
Summary: The transcriptional activity of beta-catenin plays a crucial role in the malignant progression of breast cancer, affecting tumor growth, invasion, and metastasis formation. This contrasts its adhesion function, highlighting the importance of Wnt/beta-catenin-dependent transcription in cancer progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell & Tissue Engineering
Xiaoli Shi, Yunzhu Cen, Liying Shan, Lijie Tian, Endong Zhu, Hairui Yuan, Xiaoxia Li, Ying Liu, Baoli Wang
Summary: The study revealed that NDRG1 negatively regulated cell growth and modulated the differentiation of marrow stromal progenitor cells towards adipocytes, impacting the osteoblast differentiation. NDRG1 interacted with LRP6 and inactivated the canonical Wnt/beta-catenin signaling cascade in stromal progenitor cells. In vivo transfection of Ndrg1 siRNA prevented inactivation of Wnt signaling and improved osteoblasts reduction and fat accumulation in ovariectomized mice.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Yunok Oh, Chang-Bum Ahn, M. P. C. K. Marasinghe, Jae-Young Je
Summary: Chitosan, a naturally occurring biodegradable polymer, demonstrates osteogenic effects in murine bone marrow-derived mesenchymal stem cells. Grating gallic acid onto chitosan enhances its potential for osteogenesis. Mechanistic study shows that GAC activates the Wnt signaling pathway to promote osteoblast differentiation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Irina Giralt, Gabriel Gallo-Oller, Natalia Navarro, Patricia Zarzosa, Guillem Pons, Ainara Magdaleno, Miguel F. Segura, Constantino Sabado, Raquel Hladun, Diego Arango, Jose Sanchez de Toledo, Lucas Moreno, Soledad Gallego, Josep Roma
Summary: The study demonstrates the therapeutic potential of DKK-1 pharmaceutical inhibition in rhabdomyosarcoma, resulting in beta-catenin activation and modulation of focal adhesion kinase, with positive effects on myogenic marker expression and a reduction in proliferation and invasion. In addition, the chemical inhibitor WAY-262611 was able to impair tumor cell survival in vivo, suggesting DKK-1 as a potential molecular target for novel therapeutic strategies in RMS patients, especially those with high DKK-1 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xinwei Chen, Xuzhuo Chen, Rui Chao, Yexin Wang, Yi Mao, Baoting Fan, Yaosheng Zhang, Weifeng Xu, An Qin, Shanyong Zhang
Summary: This study demonstrates the important role of Dlk2 in osteoclast development and bone homeostasis, as well as the interaction between Dlk2 and the Syap1 signaling pathway. The results indicate that Dlk2 deletion inhibits osteoclast formation and leads to a high-bone-mass phenotype, with increased bone mass observed in ovariectomized mice.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Hairui Yuan, Mengyue Li, Xue Feng, Endong Zhu, Baoli Wang
Summary: miR-142a-5p plays a critical role in osteoblast differentiation by targeting NFIA and being regulated by the Wnt/beta-catenin signaling pathway.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ashish Kumar Tripathi, Divya Rai, Priyanka Kothari, Pragati Kushwaha, Koneni Sashidhara, Ritu Trivedi
Summary: A compound, 4e, has been identified to have osteogenic potential in glucocorticoid induced osteoporosis (GIOP). Compound 4e preserved osteoblast differentiation and increased the expression of osteogenic genes, resulting in the preservation of bone microarchitecture and cortical thickness. This study is important for the treatment of glucocorticoid-induced osteoporosis.
Article
Developmental Biology
Sujie Hu, Sisi Chen, Haozu Zeng, Xinyi Ruan, Xinyi Lin, Rexhina Vlashi, Chenhe Zhou, Haidong Wang, Guiqian Chen
Summary: This study identified Ap-213 as a transcriptional factor with higher expression in frontal bone compared to parietal bone. It regulates osteoblast proliferation and differentiation through the Wnt/13-catenin signaling pathway. Ap-213 plays an important role in regulating regional osteogenic potential in frontal and parietal bone.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Morgane Le Rolle, Filippo Massa, Pam Siggers, Laurent Turchi, Agnes Loubat, Bon-Kyoung Koo, Hans Clevers, Andy Greenfield, Andreas Schedl, Marie-Christine Chaboissier, Anne-Amandine Chassot
Summary: This study shows that germ cell-intrinsic beta-catenin activity in mouse embryonic ovaries is essential for maintaining pluripotency and its repression is crucial for differentiation and meiosis entry. The interaction of beta-catenin with the pluripotent-associated factor POU5F1 in the nucleus is associated with germ cell pluripotency, and its exit from the nucleus correlates with germ cell differentiation. These findings highlight the role of beta-catenin as a central gatekeeper in ovarian differentiation and gametogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
I. Saranya, R. L. Akshaya, N. Selvamurugan
Summary: Bone remodeling involves the coordination of ossification and bone resorption activities by osteoblasts and osteoclasts, regulated by various growth factors and hormones. Dysregulation in the Wnt signaling pathway can lead to bone disorders, with non-coding RNAs playing a critical role in modulating the Wnt cascade for bone homeostasis. Understanding the regulatory role of ncRNAs could provide new therapeutic strategies for bone-related disorders.
Article
Biodiversity Conservation
Marit E. Kragt, Eric Hay, Gabriela Scheufele, Jeff Bennett, Michael Renton
JOURNAL OF APPLIED ECOLOGY
(2020)
Article
Cell Biology
Lauren E. Surface, Damon T. Burrow, Jinmei Li, Jiwoong Park, Sandeep Kumar, Cheng Lyu, Niki Song, Zhou Yu, Abbhirami Rajagopal, Yangjin Bae, Brendan H. Lee, Steven Mumm, Charles C. Gu, Jonathan C. Baker, Mahshid Mohseni, Melissa Sum, Margaret Huskey, Shenghui Duan, Vinieth N. Bijanki, Roberto Civitelli, Michael J. Gardner, Chris M. McAndrew, William M. Ricci, Christina A. Gurnett, Kathryn Diemer, Fei Wan, Christina L. Costantino, Kristen M. Shannon, Noopur Raje, Thomas B. Dodson, Daniel A. Haber, Jan E. Carette, Malini Varadarajan, Thijn R. Brummelkamp, Kivanc Birsoy, David M. Sabatini, Gabe Haller, Timothy R. Peterson
SCIENCE TRANSLATIONAL MEDICINE
(2020)
Article
Orthopedics
M. Maumus, D. Noel, H. K. Ea, D. Moulin, M. Ruiz, E. Hay, X. Houard, D. Cleret, M. Cohen-Solal, C. Jacques, J-Y Jouzeau, M-H Lafage-Proust, P. Reboul, J. Sellam, C. Vinatier, F. Rannou, C. Jorgensen, J. Guicheux, F. Berenbaum
OSTEOARTHRITIS AND CARTILAGE
(2020)
Article
Orthopedics
P. Seifer, E. Hay, L. Fleischhauer, J. Heilig, W. Bloch, S. Sonntag, D. Shmerling, H. Clausen-Schaumann, A. Aszodi, A. Niehoff, M. Cohen-Solal, M. Paulsson, R. Wagener, F. Zaucke
Summary: The study found that the matrilin-3 T298M mutation affects endochondral ossification, resulting in larger cartilage collagen fibril diameters, which increases the compressive stiffness of articular cartilage and aggravates osteoarthritis development. This mutation predisposes mice to post-traumatic osteoarthritis and provides a valid model for investigating pathogenic mechanisms involved in osteoarthritis development.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Biochemistry & Molecular Biology
Morgane Bourmaud, Mylene Zarka, Romain Le Cozannet, Pascale Fanca-Berthon, Eric Hay, Martine Cohen-Solal
Summary: The study found that Rubus idaeus extracts have a protective effect on chondrocyte metabolism and cartilage loss, reducing chondrocyte hypertrophy and cartilage breakdown. The extract also prevented the loss of proteoglycan and inhibited the expression of the NITEGE neoepitope. In an inflammatory environment, Rubus idaeus extracts can also reduce the expression of proinflammatory factors.
Article
Medicine, Research & Experimental
Mylene Zarka, Francois Etienne, Morgane Bourmaud, Denis Szondi, Jean-Marc Schwartz, Kristine Kampmann, Christophe Helary, Francois Rannou, Eric Hay, Martine Cohen-Solal
Summary: The authors investigated the role of Yes associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) in osteocyte mechanotransduction. Using a 3D osteocyte compression model, they demonstrated that YAP/TAZ signaling is activated, essential for osteocyte mechano-responsiveness, and regulates gene expression profiles through RNAseq analysis and control of chemokine expression.
LABORATORY INVESTIGATION
(2021)
Article
Orthopedics
C. Cherifi, A. Latourte, S. Vettorazzi, J. Tuckermann, S. Provot, H. -K. Ea, A. Ledoux, J. Casas, O. Cuvillier, P. Richette, A. Ostertag, E. Hay, M. Cohen-Solal
Summary: The osteoclast secretome promotes chondrocyte catabolism by increasing expression of Mmp3 and Mmp13 and activating the JNK signaling pathway, leading to matrix degradation. Inhibition of osteoclast-mediated chondrocyte catabolism with JTE013 protects against OA, indicating that osteoclastic S1P contributes to cartilage damage. S1P is identified as a potential therapeutic target in OA.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Review
Oncology
Guillaume Anthony Odri, Joelle Tchicaya-Bouanga, Diane Ji Yun Yoon, Dominique Modrowski
Summary: Osteosarcomas are heterogeneous bone tumors with complex genetic and chromosomic alterations. The metastatic capacities of osteosarcoma cells can be predicted by gene and microRNA signatures. Current therapeutic strategies have limited efficacy for metastatic osteosarcoma patients. Recent studies suggest a hierarchical organization of tumor cell clones and the potential involvement of cancer stem cells in metastatic progression.
Review
Cell Biology
Mylene Zarka, Eric Hay, Martine Cohen-Solal
Summary: YAP and TAZ are key regulators in organ growth and bone biology, responding to mechanical and cytoskeletal cues to control cell fate. They play significant roles in bone and cartilage homeostasis and their precise functions have been investigated in mouse and cellular models.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Joelle Tchicaya-Bouanga, Yu-Jen Hung, Jean-Marc Schwartz, Diane Ji Yun Yoon, Emilie Chotard, Clarice Marty, Guillaume Anthony Odri, Gonzague de Pinieux, Martine Cohen-Solal, Dominique Modrowski
Summary: This study reveals the involvement of calpain-6 in promoting tumor growth and metastasis in sarcoma stem cells. Calpain-6 modulates the expression of Hippo pathway genes and stabilizes YAP, as well as regulates the vesicular trafficking of beta-catenin degradation complexes. The expression of calpain-6 is associated with genes related to the G2M phase of the cell cycle and up-regulated genes controlling mitosis in sarcoma stem cells and tissues. In mouse models, YAP inhibition prevents the formation of primary tumors and metastases, but has no effect on already established tumors and can even accelerate lung metastasis associated with large bone tumors.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Yohan Jouan, Zohra Bouchemla, Benoit Bardeche-Trystram, Joanna Sana, Caroline Andrique, Hang-Korng Ea, Pascal Richette, Augustin Latourte, Martine Cohen-Solal, Eric Hay
Summary: This study reveals a new pathway involving the Lin28a-Let7 axis that promotes chondrocyte metabolism in injured cartilage. It contributes to the understanding of cartilage physiology and the pathogenesis of osteoarthritis.
Meeting Abstract
Cell Biology
Sherine Moustaghfir, Marielle Pasdeloup, Eric Hay, Hang-Korng Ea, Martine Cohen-Solal, Roland. Schule, Frederic Mallein-Gerin, Jerome E. Lafont
WOUND REPAIR AND REGENERATION
(2022)
Meeting Abstract
Orthopedics
S. Moustaghfir, M. Pasdeloup, E. Hay, H. -K. Ea, M. Cohen-Solal, R. Schuele, F. Mallein-Gerin, J. Lafont
OSTEOARTHRITIS AND CARTILAGE
(2022)
Meeting Abstract
Orthopedics
S. Brochard, J. Aury-Landas, V. Agin, B. Bernay, J. Pontin, C. Seillier, O. Toutirais, M. Cohen-Solal, E. Hay, K. Boumediene, C. Bauge
OSTEOARTHRITIS AND CARTILAGE
(2022)
Meeting Abstract
Orthopedics
S. Moustaghfir, M. Pasdeloup, E. Hay, H. -K. Ea, M. Cohen-Solal, R. Schuele, E. Perrier-Groult, F. Mallein-Gerin, J. E. Lafont
OSTEOARTHRITIS AND CARTILAGE
(2021)