Journal
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
Volume 33, Issue 12, Pages 4023-4027Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.5012/bkcs.2012.33.12.4023
Keywords
Microfluidic image cytometry; Microfluidic device; Cytotoxicity assessment; Ag nanoparticles; Nanotoxicity
Categories
Funding
- Korea Science and Engineering Foundation (KOSEF)
- Ministry of Education, Science and Technology (MEST) [2008-02968]
- National Research Foundation of Korea [2008-02968] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (mu FIC). The LC50 value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/L). These results confirm that the mu FIC approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This mu FIC method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty- toxicity relationships.
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