Article
Multidisciplinary Sciences
M. K. Prasannakumar, P. Buela Parivallal, Devanna Pramesh, H. B. Mahesh, Edwin Raj
Summary: The study developed a microdevice platform using LAMP technology to detect Magnaporthe oryzae and Sarocladium oryzae in rice seeds, showing high sensitivity and potential for on-field diagnostic kits to rapidly diagnose plant pathogens.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Qing Fan, Zhixun Xie, You Wei, Yanfang Zhang, Zhiqin Xie, Liji Xie, Jiaoling Huang, Tingting Zeng, Sheng Wang, Sisi Luo, Meng Li
Summary: Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique that offers convenience, specificity, and sensitivity. However, previous LAMP detection methods have been limited to identifying a single target due to the interpretation methods. In this study, a modified LAMP method was developed that allows for the simultaneous detection of multiple target sequences.
Article
Multidisciplinary Sciences
Jiaojiao Gong, Guanghui Zhang, Wangguo Wang, Liping Liang, Qianyun Li, Menghao Liu, Liang Xue, Guanghui Tang
Summary: The study proposed a non-genotyping method for detecting 13 types of high-risk HPV in a single reaction by combining isothermal recombinase polymerase amplification (RPA) with CRISPR-Cas12a technology. The result could be achieved in 35 minutes with high sensitivity (500 copies per reaction), representing significant advances for the application of the RPA-Cas12a system and holding great potential to address key challenges in HPV diagnostics.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Haoqing Zhang, Zhiqiang Yan, Xinlu Wang, Martina Ganova, Honglong Chang, Sona Lassakova, Marie Korabecna, Pavel Neuzil
Summary: This study introduced a single-channel qPCR duplexing method using EvaGreen intercalating dye and FAM probe, offering a cost-effective alternative to probe-based qPCR. The method demonstrated the ability to determine detectable concentration ratios of targets and eliminate fluorescence overspilling, while confirming PCR product specificity through melting curve analysis. The approach was validated on a commercial thermal cycler, increasing its utility for researchers in need of a practical and economical molecular diagnostics tool.
Article
Chemistry, Multidisciplinary
Xiaojing Wang, Skye Shepherd, Nantao Li, Congnyu Che, Tingjie Song, Yanyu Xiong, Isabella Rose Palm, Bin Zhao, Manish Kohli, Utkan Demirci, Yi Lu, Brian T. T. Cunningham
Summary: In this study, an efficient target recycling amplification process (TRAP) using photonic resonator absorption microscopy was developed for the digital detection of exosomal microRNAs (miRNAs). The TRAP approach demonstrated high sensitivity, robust selectivity, and a broad dynamic range, making it ideal for quantifying miRNA biomarkers in low concentrations or sample volume. Compared to traditional qRT-PCR, TRAP showed similar accuracy in profiling exosomal miRNAs derived from cancer cells, but with significantly enhanced detection limits for miRNA-375 and miRNA-21.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Dayong Li, Chengjie Duan, Wenting Cheng, Youjing Gong, Yanheng Yao, Xiaoping Wang, Zhongyun Wang, Yang Xiang
Summary: A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation from the target sequence, demonstrating its superior analytical performance.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Qiang Liu, Mei Liu, Yan Jin, Baoxin Li
Summary: Fluorescence biosensing based on CRISPR/Cas12a system with ratiometric probe as the reporter exhibits higher sensitivity compared to traditional TaqMan probe, and it can distinguish and avoid false positive signals.
Article
Spectroscopy
Yu Peng, Mingming Xu, Yushuang Guo, Hualin Yang, Yu Zhou
Summary: A novel signal amplification biosensor based on asymmetric PCR was used to detect Cd2+. The asymmetric PCR obtained a higher level of fluorescent signal than symmetric PCR. The biosensor exhibited a low detection limit, wide linear range, and satisfactory selectivity and recovery rate.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Yujun Xing, Jie Liang, Fei Dong, Jirong Wu, Jianrong Shi, Jianhong Xu, Jinke Wang
Summary: We developed a novel loop-mediated isothermal amplification (LAMP) method using DNA captured on polyacrylamide microparticles (PAMMPs) as templates (PAMMPs@DNA-LAMP) for rapid qualitative detection of genetically modified organisms (GMOs). The PAMMPs@DNA-LAMP method allowed for rapid DNA extraction (5-10 min) and amplification (around 30 min) at a constant temperature of 63°C. The DNA captured by PAMMPs could be effectively detected by both conventional and quantitative PCR (qPCR) and LAMP.
Article
Chemistry, Analytical
Chao Zhang, Tingting Zheng, Hua Wang, Wei Chen, Xiaoye Huang, Jianqi Liang, Liping Qiu, Da Han, Weihong Tan
Summary: This study developed a rapid detection method for SARS-CoV-2, with the entire sample-to-result workflow taking less than 40 minutes. The method achieved 100% accuracy compared to PCR-based assays, offering a potential universal platform for rapid and point-of-care detection of emerging infectious diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Kittisak Buddhachat, Suphaporn Paenkaew, Nattaporn Sripairoj, Yash Munnalal Gupta, Waranee Pradit, Siriwadee Chomdej
Summary: The combination of DNA barcodes and CRISPR-cas system provides a fast and accurate method for species authentication, offering high specificity, sensitivity, speed, and simplicity for plant species identification.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Yaqian An, Zhiqi Yu, Di Liu, Lirong Han, Xian Zhang, Xuelian Xin, Cuiping Li
Summary: A sensitive and label-free detection strategy for M.SssI MTase activity was proposed using a HpaII-assisted and linear amplification-enhanced exponential amplification strategy. The method showed good selectivity against other types of MTases and can be extended to the analysis of other DNA MTases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuanyang Yao, Shuhao Zhao, Yan Liang, Fei Hu, Niancai Peng
Summary: This study improved a one-stage deep learning algorithm called YOLOv5, applied it to the detection of droplets in dPCR images, and achieved a higher detection rate for small targets. A new loss function was used to speed up the training process, and a network pruning method was employed to enable deployment on mobile devices while maintaining performance. The improved model accurately identified droplets with an error rate of 0.65% and showed fast detection speed, high accuracy, and suitability for mobile devices or cloud platforms.
Article
Multidisciplinary Sciences
Yusuke Kimura, Masashi Ikeuchi
Summary: In this study, a non-electrically controlled saliva testing method was developed to detect viral infections by amplifying viral DNA expression in saliva. This method has high specificity and a low detection limit for viral concentration, allowing for early detection of viral infections before the onset of symptoms.
SCIENTIFIC REPORTS
(2023)
Review
Chemistry, Multidisciplinary
Ofer I. Wilner, Doron Yesodi, Yossi Weizmann
Summary: The COVID-19 pandemic has highlighted the urgent need for quick, easy-to-use, reliable, and affordable diagnostic tests and devices at various locations. This paper introduces PCR and isothermal amplification reactions, reviews commercially available point-of-care nucleic acid diagnostic devices, and discusses efforts in developing reliable, portable, cost-effective, and automatic point-of-care nucleic acid diagnostic devices. Its purpose is to bridge the gap between academia and industry and share important knowledge on this topic.
