Sensitive and simple detection of trace hydrogen peroxide based on a resonance light scattering assay

Under weak acidic conditions, with haemoglobin (Hb) as a mimetic peroxidase, the oxidation reaction between H2O2 and Kl was cataEysed by haemogEobin to form 12, which combined with excessive I- to form I-3(-). I-3(-) then reacted with gold nanoparticles (AuNPs) to produce Aul(2)(-), which decreased the resonance Eight scattering intensity of the gold nanoparticles at 575 nm. The resonance Eight scattering (RLS) intensity linearly decreased as the hydrogen peroxide concentration increased. On this basis, a new method for the resonance Eight scattering determination of hydrogen peroxide has been developed, and the optimum reaction conditions, influential factors and applications were investigated. Under the optimal reaction conditions, hydrogen peroxide concentrations of 3.25 x 10(-7)-2.60 x 10(-5) mol L-1 were EinearEy correEated with the reduction in the resonance Eight scattering intensity (Delta l) with a correEation coefficient of 0.9992 and a detection Eimit of 2.48 x 10(-7) moE L-1 The new method has been applied to the analysis of hydrogen peroxide in milk samples with satisfactory results.