Article
Cell Biology
Jia Wang, Yue Sun, Yiping Liu, Jize Yu, Xiaolin Sun, Lin Wang, Yanmin Zhou
Summary: This study investigated the existence of MSCs in Schneiderian membrane and the effects of PRF on these cells. The results showed that MSCs existed in Schneiderian membrane and PRF stimulation promoted cell proliferation, migration, and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation could accelerate bone healing and increase the quantity and quality of new bone.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Kostina, Arseniy Lobov, Daria Semenova, Artem Kiselev, Polina Klausen, Anna Malashicheva
Summary: Despite significant progress in bone tissue regeneration, the early mechanisms of osteogenic differentiation remain poorly understood. Various cells can undergo osteogenic transformation, with Notch signaling playing a key role in this process. Research demonstrates differences in the ability of different cell types to undergo Notch-dependent osteogenic differentiation.
Article
Plant Sciences
Avinash Sanap, Kalpana Joshi, Tejas Shah, Girish Tillu, Ramesh Bhonde
Summary: The study showed that Piper longum L. fruit extract significantly increased proliferation of WJMSCs and enhanced Ca2+ accumulation and matrix mineralization, along with promoting alkaline phosphatase (ALP) activity and the expression of key gene mRNAs. This suggests that the fruit extract can enhance osteogenic differentiation of WJMSCs, potentially serving as an anti-osteoporotic agent.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Pharmacology & Pharmacy
Jinli Zhang, Zhihe Liu, Yang Luo, Xiaojian Li, Guowei Huang, Huan Chen, Aiguo Li, Shengnan Qin
Summary: Flavonoids have the potential to regulate the osteogenic differentiation of mesenchymal stem cells (MSCs), making them a promising therapeutic application in bone disorders and bone tissue engineered constructs.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yanjun Lin, Min Zhang, Lin Zhou, Xuxi Chen, Jiang Chen, Dong Wu
Summary: The study found that rapamycin (RAPA) can promote osteogenic differentiation of maxillary sinus membrane stem cells (MSMSCs), indicating a possible relationship between osteogenic differentiation and autophagy. MSMSCs showed active proliferation and multi-differentiation capacity, displaying a phenotype of mesenchymal stem cells. Alkaline phosphatase (ALP) activity and calcium nodules formation in groups treated with four different concentrations of RAPA at different time points showed significant differences. In vivo, 100 nM and 10 nM RAPA-treated groups exhibited more bone formation.
Article
Biochemistry & Molecular Biology
Chanchao Lorthongpanich, Thanapon Charoenwongpaiboon, Prapasri Supakun, Methus Klaewkla, Pakpoom Kheolamai, Surapol Issaragrisil
Summary: The study showed that fisetin can inhibit proliferation, migration, and osteogenic differentiation of human MSCs and SaOs-2 cells by reducing YAP activity, leading to downregulation of osteogenic genes and upregulation of fibroblast genes. Molecular docking and simulations suggest that fisetin may disrupt protein-protein interactions by occupying the TEAD pocket, indicating its potential role in regulating human osteogenesis.
Article
Chemistry, Physical
Jing Liu, Yaya Cheng, Haoyu Wang, Dingyi Yang, Cunshun Liu, Weixin Dou, Xue Jiang, Hongzhang Deng, Rusen Yang
Summary: Researchers developed a composite piezoelectric fibrous membrane with titanium dioxide nanoparticles and PVDF for cellular osteogenic differentiation. The membrane significantly promoted the expression of osteocalcin gene and cell adhesion and proliferation through electromechanical stimulation. The proposed mechanism provides guidance for designing electroactive materials to promote osteogenic differentiation and bone regeneration.
Article
Biochemistry & Molecular Biology
Jiang Liu, Xin-Tong Yao, Xiao-Lei Feng, Xi-Zhuang Bai
Summary: In recent years, there has been increasing attention on bone loss related diseases, such as osteoporosis and osteonecrosis of the femoral head, which exhibit symptoms of insufficient bone mass. Mesenchymal stem cells (MSCs) hold potential as a new solution for bone disease, as they can be induced to differentiate into osteoblasts. This study investigates the mechanism by which BMP2 drives MSCs to differentiate into osteoblasts through ACKR3/p38/MAPK signaling. The findings suggest that ACKR3 could be a novel therapeutic target for bone-associated diseases and bone-tissue engineering.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Toxicology
AtLee T. D. Watson, Aldo Carmona Baez, Dereje Jima, David Reif, Jun Ding, Reade Roberts, Seth W. Kullman
Summary: This study aims to investigate the regulatory mechanisms of osteoblast differentiation through global transcriptomic analysis of human bone-derived mesenchymal stem cells (hBMSCs) exposed to TCDD. The results show that TCDD exposure attenuates osteoblast differentiation of hBMSCs and leads to altered gene expression.
TOXICOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
