Article
Biochemistry & Molecular Biology
Iraj Hashemzadeh Segherloo, Jorg Freyhof, Patrick Berrebi, Anne-Laure Ferchaud, Matthias Geiger, Jerome Laroche, Boris A. Levin, Eric Normandeau, Louis Bernatchez
Summary: Defining the taxonomic status of recently radiated groups poses challenges due to low phylogenetic signal, with the study on the brown trout species-complex revealing natural hybridization playing a key role in the origin of some putative species. The study suggests a multi-species taxonomy for brown trouts and the need for an integrative approach combining genomic, morphological, and ecological data for a comprehensive understanding of species complexes.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2021)
Article
Multidisciplinary Sciences
Natalia Kurhaluk, Halyna Tkachenko
Summary: This study investigated the effects of different development stages, survival forms, and sex on the functional stability of sea trout muscle tissue. It found sex-related relationships in antioxidant balance and muscle tissue degradation in the adult stage, as well as modifications in lysosomal functioning due to long-term environmental stress.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Marius Pedersen, Ahmed Mohammed
Summary: The study presents an automatic method for individual brown trout identification using deep convolutional architecture, achieving a precision of 94.6% and a recall of 74.3%.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Carmine Merola, Angelo Bisegna, Giovanni Angelozzi, Annamaria Conte, Maria Cesarina Abete, Caterina Stella, Sabina Pederiva, Caterina Faggio, Nicoletta Riganelli, Monia Perugini
Summary: This research investigated the concentrations of heavy metals and levels of vitellogenin in wild brown trout from two different rivers in the Abruzzi region of Italy. The results showed that the water quality in both rivers is suitable for brown trout life, and there were no significant differences in metal concentrations and VTG levels between the trout from the two rivers.
APPLIED SCIENCES-BASEL
(2021)
Article
Fisheries
Shuaijie Sun, Zhitong Wang, Dongdong Yuan, Mengke Ni, Huifen Xu, Wanliang Wang, Chi Zhang, Meiqun Chen, Lamu ZhaXi, Jianshe Zhou, Ming Li
Summary: This study used RT-qPCR technology to investigate the expression of eight candidate genes in different tissues, developmental stages, and under bacterial stress in Salmo trutta. The stability of these genes was ranked using comprehensive analysis software. The results showed that the expression of the hprt1 gene was relatively stable under different conditions.
AQUACULTURE REPORTS
(2022)
Article
Veterinary Sciences
Agata Jozefiak, Mateusz Rawski, Bartosz Kieronczyk, Damian Jozefiak, Jan Mazurkiewicz
Summary: This study evaluated the effects of two diets containing mealworm and superworm on the gut microbiome of sea trout fingerlings. The results showed that both insect meals did not have a significant impact on the growth and survival rate of sea trout. However, there were noticeable microbiome differences at lower taxonomic levels, particularly with the superworm meal.
BMC VETERINARY RESEARCH
(2023)
Article
Fisheries
Gasper Renko, Ida Djurdjevic, Simona Susnik Bajec
Summary: A novel protocol for the isolation of chromatophores from brown trout skin was developed based on previous research on zebrafish, enabling specific gene expression evaluation. The method is simple, fast, and ethical, allowing for monitoring at multiple time points, and should be effective for the majority of salmonid species due to similarities in skin morphology.
Review
Chemistry, Multidisciplinary
Edo D'Agaro, PierPaolo Gibertoni, Fabio Marroni, Maria Messina, Emilio Tibaldi, Stefano Esposito
Summary: Salmonid fish, particularly brown trout, are widely used as ecological and research models in the conservation genetics and genomics field. The Salmo trutta complex, which consists of multiple geographic forms, is found in Europe. Seven major evolutionary lineages have been identified based on mitochondrial DNA analysis. Populations in Italy and surrounding areas show high genetic diversity, and some lineages are protected due to their threatened status. Recent studies using traditional and next-generation sequencing techniques have revealed genetic differences between populations in different regions.
APPLIED SCIENCES-BASEL
(2022)
Article
Multidisciplinary Sciences
Rafal Bernas, Anna Was-Barcz, Mariann Arnyasi, Piotr Debowski, Grzegorz Radtke, Anita Pocwierz-Kotus, Patrick Berrebi
Summary: Selection, genetic drift, and gene flow affect genetic variation within populations and genetic differences among populations. In brown trout populations, disrupted river-segment connectivity is the main factor affecting population fragmentation. The study found a complex pattern of gene flow between resident and migratory brown trout, with isolated resident specimens sharing spawning grounds with sea trout downstream of impassable barriers.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Anna Monnolo, Maria Teresa Clausi, Filomena Del Piano, Mario Santoro, Maria Lorena Fiorentino, Lorella Barca, Giovanna Fusco, Barbara Degli Uberti, Luigia Ferrante, Raffaelina Mercogliano, Maria Carmela Ferrante
Summary: This study analyzed wild brown trout samples from a protected area in Southern Italy and found variations in the concentration of gastro-intestinal helminths and organochlorine pollutants among the samples. The results suggest that organochlorine pollutants may affect parasite survival and contribute to the understanding of the interaction among environment, parasites, and host.
Article
Fisheries
Lilianna Hoffmann, Mateusz Rawski, Ewa Pruszynska-Oszmalek, Pawel Kolodziejski, Jan Mazurkiewicz
Summary: The study showed that diets containing insect meal from the larval stage have a positive impact on the growth and survival rate of fish compared to control diets and diets containing insect meal from the imago stage.
AQUACULTURE REPORTS
(2021)
Article
Marine & Freshwater Biology
Zivile Jurgelene, Danguole Montvydiene, Saulius Stakenas, Justas Poviliunas, Simonas Rackauskas, Ricardas Taraskevicius, Vesta Skrodenyte-Arbaciauskiene, Nijole Kazlauskiene
Summary: This study investigates the effects of different brands of alizarin dye on fish health and finds significant differences between brands. The results demonstrate that the toxicity of the dye varies depending on its concentration and the developmental stage of the fish.
AQUATIC TOXICOLOGY
(2022)
Article
Microbiology
Katharina Keiz, Sebastian Ulrich, Jasmin Wenderlein, Patrick Keferloher, Anna Wiesinger, Klaus Neuhaus, Ilias Lagkouvardos, Helmut Wedekind, Reinhard K. Straubinger
Summary: The bacterial community of brown trout from unfertilized egg to 95 days post fertilization (dpf) was studied using 16S rRNA gene amplicon sequencing. Significant changes in bacterial richness, microbial diversity, and taxonomic composition were observed during early developmental stages until 67 dpf, while the bacterial community seemed to stabilize in the fry stages from 67 to 95 dpf.
Article
Environmental Sciences
Dhamotharan Kannimuthu, HyeongJin Roh, H. Craig Morton, Ma Michelle D. Penaranda, Anne Vossgard, Tom Hansen, Per Gunnar Fjelldal, Egil Karlsbakk, Ingrid Fiksdal, Maria K. Dahle, Hakon Berg-Rolness, Stig Maehle, Ghebretnsae Dawit Berhe, Joachim Nordbo, Sonal Patel, Abdullah Madhun, Soren Grove, Bjorn Olav Kvamme
Summary: PRV-1 can infect all developmental stages of Atlantic salmon and brown trout, but brown trout have a lower infection prevalence. All life stages of Atlantic salmon develop heart lesions characteristic of HSMI, while brown trout only exhibit mild infections without pathological changes in target organs. These results strongly suggest that brown trout are less susceptible to PRV-1 infection compared to Atlantic salmon, confirming the species-specific susceptibility and disease development for PRV-1 infection.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Katarzyna Dziewulska, Lucyna Kirczuk, Robert Czerniawski, Monika Kowalska-Goralska
Summary: The study demonstrates that selenium supplementation of fertilized eggs can improve breeding outcomes in fish in selenium-poor areas. Adequate selenium intake benefits the health and growth of organisms, while overdose can be harmful. Selenium concentration should be adjusted based on the chemical compound, fish species, and selenium content in the yolk.
Article
Environmental Sciences
Ariana Perez-Pereira, Claudia Ribeiro, Filomena Teles, Ricardo Goncalves, Virginia M. F. Goncalves, Jose Augusto Pereira, Joao Soares Carrola, Carlos Pires, Maria Elizabeth Tiritan
Summary: This study separated the enantiomers of ketamine and norketamine and investigated their toxicity in different aquatic organisms. The results showed that at high concentrations, the toxicity of ketamine was enantioselective for both organisms.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2022)
Article
Immunology
Jessica Almeida, Claudia A. S. Araujo, Bartira De Aguiar Roza, Marina Martins Siqueira, Eduardo Rocha
Summary: This study aimed to identify and assess the main risks of the organ donation-transplantation process in Brazil, with most risks related to insufficient human and material resources, lack of staff training and commitment, and poor infrastructure or logistical aspects. The findings provide a basis for future studies to delve deeper into each of these risks and address the critical weaknesses in the process.
TRANSPLANTATION PROCEEDINGS
(2021)
Article
Environmental Sciences
Ondina Ribeiro, Luis Felix, Claudia Ribeiro, Bruno Castro, Maria Elizabeth Tiritan, Sandra Mariza Monteiro, Joao Soares Carrola
Summary: This study investigated the toxicity effects and possible enantioselective effects of venlafaxine on daphnia and zebrafish. The results showed that venlafaxine is not expected to induce harmful effects at environmental levels, but one enantiomer, (R)-VEN, increased malformations in zebrafish larvae.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2022)
Review
Environmental Sciences
Maria Joao Rocha, Eduardo Rocha
Summary: This review summarizes the concentrations of 12 synthetic progestins (PGs) in wastewater treatment plants and other matrices. PGs have been found to affect the reproductive fitness and development of biota, particularly in fish. However, research on the environmental concentrations and effects of PGs is still limited.
Article
Environmental Sciences
Maria Joao Rocha, Frederico Silva, Eduardo Rocha
Summary: The concentrations of seventeen endocrine disruptor compounds were investigated in the Douro River estuary, showing the widespread presence of these substances. High levels of oestrogens, phytoestrogens, and industrial pollutants were found, indicating ongoing risk of endocrine disruption for the local aquatic wildlife. The physicochemical data suggest direct sewage discharges, which may pose risks to humans through bathing and consumption of local fish.
Article
Environmental Sciences
Fernanda Malhao, Ana Catarina Macedo, Alice Abreu Ramos, Eduardo Rocha
Summary: This study aimed to generate multicellular aggregates using ultra-low attachment plates and characterize them through morphometry, qualitative cytology, and quantitative immunocytochemistry analysis. The different cell lines showed structural differences in their generated MCAs and had varying results in the ICC analysis.
Article
Agriculture, Dairy & Animal Science
Sukanlaya Tantiwisawaruji, Maria J. Rocha, Ana Silva, Miguel A. Pardal, Uthaiwan Kovitvadhi, Eduardo Rocha
Summary: This study conducted a quantitative microscopic examination of the ganglia of adult peppery furrow shell. The results showed that females have a larger glia-to-neuron numerical ratio and greater ganglionic volume compared to undifferentiated adults and males. The research also revealed differences in ganglion types and their total volumes and volume ratios. These findings provide valuable insight into the neurophysiology of bivalves.
Article
Marine & Freshwater Biology
Ines L. Pereira, Celia Lopes, Eduardo Rocha, Tania V. Madureira
Summary: This study optimized a 3D model of brown trout hepatocyte spheroids and used it to investigate the effects of androgens on lipidic target genes. The results suggest that DHT modulates distinct lipidic pathways in brown trout and demonstrate that this 3D model is a valuable tool for physiological and toxicological mechanistic studies.
AQUATIC TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rodrigo F. Alves, Eduardo Rocha, Tania Madureira
Summary: In vitro fish cell cultures are alternative models for in vivo toxicological studies. Two-dimensional cultures have limitations in longevity and maintaining in vivo cellular functions, and three-dimensional systems are proposed to better mimic in vivo effects. Fish hepatocyte spheroids, derived from liver cell lines or primary cultures, are the most used 3D model in fish. They closely resemble in vivo counterparts and have higher metabolic capacity compared to monolayer cultures.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ana Rita Costa, Virginia M. F. Goncalves, Bruno B. Castro, Joao Soares Carrola, Ivan Langa, Ariana Pereira, Ana Rita Carvalho, Maria Elizabeth Tiritan, Claudia Ribeiro
Summary: This study investigated the ecotoxicological effects of MDMA and its enantiomers in Daphnia magna. The results showed that sub-chronic exposure to MDMA racemate and its enantiomers can interfere with the morphophysiology and swimming behavior of D. magna. The (R)-enantiomer was found to be less toxic than the (S)-enantiomer.
Article
Environmental Sciences
Claudia Ribeiro, Cristiano Gomes, Ariana Perez-Pereira, Joao Soares Carrola, Maria Elizabeth Tiritan, Ivan Langa, Cristina Couto, Bruno B. Castro
Summary: This study aimed to investigate the enantioselective toxicity of amphetamine to the freshwater invertebrate Daphnia magna. The results showed that amphetamine had enantioselective effects on Daphnia, including impacts on growth, heart rate, swimming behavior, and reproduction. This research demonstrates the potential environmental risk of this psychoactive drug and the importance of accurate risk assessment for medium- to long-term exposure.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2023)
Article
Environmental Sciences
Maria Joao Rocha, Eduardo Rocha
Summary: The distribution patterns and amounts of 56 priority pesticides in the Ave basin were evaluated. The results showed high pesticide concentrations, which have caused significant disturbance to the ecosystem. The pesticide levels exceeded the maximum established by European legislation, posing a risk to aquatic organisms.
MARINE POLLUTION BULLETIN
(2023)
Article
Environmental Sciences
Maria Joao Rocha, Eduardo Rocha
Summary: The Sado basin, an area of intensive agricultural activity, lacks data on water levels of priority pesticides such as fungicides, herbicides, and insecticides. Water samples collected from nine sites along the Sado River Estuary were analyzed, revealing that more than 87% of the pesticides were quantified, with 42% and 72% exceeding the maximum limits established by European Directives 98/83/EC and 2013/39/EU. Fungicides, herbicides, and insecticides had average annual amounts of approximately 3.2 μg/L, 1.0 μg/L, and 12.8 μg/L, respectively. Risk assessment identified invertebrates as the most vulnerable trophic level, implicating chlorpyriphos and cyfluthrin as primary contributors based on acute in vivo assays using Daphnia magna. These findings, coupled with the high phosphate concentrations, indicate environmental and potential human health risks in the Sado waters.
Article
Agriculture, Dairy & Animal Science
Rodrigo F. Alves, Celia Lopes, Eduardo Rocha, Tania V. Madureira
Summary: Fish hepatocyte spheroids, a three-dimensional structure with similar morphofunctional properties to the liver, have shown potential as an alternative model for studying fish hepatic adaptive and toxicological responses to xenobiotics. In this study, brown trout hepatocyte spheroids were characterized at different time points up to 25 days in culture, and they exhibited stable morphology and function from the 12th to the 20th day. This model can be used for exposure tests to different xenobiotics, particularly estrogenic compounds.
Article
Environmental Sciences
Ondina Ribeiro, Claudia Ribeiro, Luis Felix, Isabel Gaivao, Joao Soares Carrola
Summary: This study evaluated the potential effects of 3-MMC on zebrafish embryonic development, behavior, and DNA integrity. The results showed that at low concentrations, 3-MMC did not have expected harmful effects on critical development stages of fish.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)