Journal
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Volume 82, Issue 3, Pages 280-284Publisher
SPRINGER
DOI: 10.1007/s00128-008-9579-2
Keywords
Fish; Endocrine disruptors; Gonads; Hormone
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Funding
- USB RIFCH [MSM6007665809]
- Czech Science Foundation [525/06/P234]
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The effects of 17 beta-estradiol (E(2)) and testosterone (T) singly and in combination were tested on juvenile chub (Leuciscus cephalus L.). Vitellogenin (VTG) and 11-ketotestosterone (11-KT) were determined by ELISA in whole body homogenates and the gonads were examined histologically. Testosterone and estradiol, in combination, significantly increased whole body VTG (p < 0.01), but not 11-KT, compared to controls and the T treated groups. The only intersex observed (1/80) was in the combined treatment group. We suggest that VTG measured in whole body homogenates could be used to determine the effects of exogenous steroids in juvenile chub.
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