Article
Neurosciences
Zhenzhou Li, Heather L. McConnell, Teresa L. Stackhouse, Martin M. Pike, Wenri Zhang, Anusha Mishra
Summary: Impairment of neurovascular coupling outside the infarct region may contribute to neurological deterioration in stroke patients, potentially linked to 20-HETE-dependent vasoconstriction. Inhibiting omega-hydroxylases may restore healthy neurovascular coupling post-stroke.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Immunology
Han-Dong Li, Ran Li, Ying Kong, Wenyan Zhang, Caiyun Qi, Dan Wang, Hongying Hao, Qiang Liu
Summary: Results from this study demonstrate that the use of a selective S1PR1 modulator, Ozanimod, significantly enhances the thrombolytic effect of a sub-thrombolytic dose of tPA, improving the therapeutic benefit for patients with acute ischemic stroke.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Surgery
Bryan Wermelink, Kirsten F. Ma, Marieke Haalboom, Mostafa El Moumni, Jean-Paul P. M. De Vries, Robert H. Geelkerken
Summary: This systematic review provides an overview of 10 tissue perfusion assessment techniques for patients with PAD. It seems too early to appoint one of them as a reference standard. Future research should focus on clinical accuracy, reliability, and validation of the techniques.
EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY
(2021)
Review
Chemistry, Inorganic & Nuclear
Vinayak Juvekar, Sang Jun Park, Juyoung Yoon, Hwan Myung Kim
Summary: Two-photon microscopy combined with two-photon fluorescent probes enables visualization of metal ion distribution in live tissues, facilitating the development of probes for metal ion detection. Recent progress between 2014 and 2020, including design strategies, history, and specific applications, was summarized in this review.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Biology
Paul Shin, Qi Pian, Hidehiro Ishikawa, Gen Hamanaka, Emiri T. Mandeville, Shuzhen Guo, Buyin Fu, Mohammed Alfadhel, Srinivasa Rao Allu, Ikbal Sencan-Egilmez, Baoqiang Li, Chongzhao Ran, Sergei A. Vinogradov, Cenk Ayata, Eng Lo, Ken Arai, Anna Devor, Sava Sakadzic, Daniel Henrion
Summary: Aging is a risk factor for cognitive impairment. Aerobic exercise benefits brain function and may promote cognitive health in older adults. This study found that aerobic exercise can improve cerebral microcirculation and cognitive function in aging individuals.
Review
Chemistry, Analytical
Hyo Won Lee, Vinayak Juvekar, Dong Joon Lee, Hwan Myung Kim
Summary: Biological events occur in specific cellular organelles to perform specialized roles in cells and collaborate with other organelles. Various methods have been used to study organelle dynamics, including two photon microscopy. This review focuses on the design strategies, photo-physical properties, and bioimaging applications of recent two-photon probes specifically localized in the endoplasmic reticulum, Golgi, lipid droplets, lysosomes, plasma membrane, and mitochondria. The summary is limited to research from 2017 onwards. © 2023 Elsevier B.V. All rights reserved.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Optics
Colin J. R. Sheppard, Marco Castello, Giorgio Tortarolo, Alessandro Zunino, Eli Slenders, Paolo Bianchini, Giuseppe Vicidomini, Alberto Diaspro
Summary: We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning, and background rejection are all improved over nonconfocal two-photon microscopy. Replacing the pinhole of confocal two-photon microscopy with a detector array increases the peak intensity of the point spread function. The outer pixels of a detector array give signals from defocused regions, and thus the processing of these, such as through subtraction, can further improve optical sectioning and background rejection.
Article
Chemistry, Analytical
Wei Yan, Yangrui Huang, Luwei Wang, Jin Li, Yong Guo, Zhigang Yang, Junle Qu
Summary: This study demonstrates a multi-color two-photon imaging method with single-wavelength excitation. The problem of spectral crosstalk is effectively solved by selecting suitable filter combinations and applying image subtraction. The results show that two-color and three-color two-photon imaging can be achieved using a single femtosecond laser.
Article
Optics
Yuezhi He, Jing Yao, Lina Liu, Yufeng Gao, Jia Yu, Shiwei Ye, Hui Li, Wei Zheng
Summary: Artificial neural networks have demonstrated proficiency in transforming low-resolution microscopic images into high-resolution images. However, the lack of training data and the need for expensive computational resources pose challenges. A self-supervised deep-learning-based volumetric imaging approach called Self-Vision is introduced, which requires no training data except for the input image itself. This lightweight network has shown resolution-enhancing power comparable to recent microscopy models, enabling large image inference with less post-processing and reducing acquisition time.
PHOTONICS RESEARCH
(2023)
Review
Chemistry, Analytical
Vinayak Juvekar, Hyo Won Lee, Dong Joon Lee, Hwan Myung Kim
Summary: Two-photon microscopy is an important tool for deep-tissue imaging, providing high-resolution fluorescence images beyond millimeter depths. Recent research has focused on developing innovative strategies and probes for quantitative analysis using two-photon fluorescence.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Luqman Khan, Rick van Lanen, Govert Hoogland, Olaf Schijns, Kim Rijkers, Dimitrios Kapsokalyvas, Marc van Zandvoort, Roel Haeren
Summary: The review highlighted the application of two-photon imaging (2PI) in epilepsy research, showcasing its ability to evaluate pathomechanisms associated with epileptic seizures, including propagation models, cellular changes, and neurovascular alterations. The findings suggest that 2PI is a valuable imaging technique in advancing our understanding of epilepsy pathophysiology.
APPLIED SCIENCES-BASEL
(2021)
Article
Optics
Sandro Heuke, Carla Silva Martins, Remi Andre, Loic Legoff, Herve Rigneault
Summary: Two-photon excited fluorescence (2PEF) microscopy is a popular nonlinear imaging method for biomedical samples. It uses multiple detectors and spectral filter sets to discriminate different fluorophores based on their emission behavior. However, 2PEF suffers from the loss of fluorescence photons outside the filter transmission range and may fail to discriminate fluorophores with similar emission profiles. Here, we propose an alternative 2PEF method that discriminates fluorophores based on their excitation spectra.
Article
Biochemistry & Molecular Biology
Rocio Fernandez-Serra, Emma Martinez-Alonso, Alberto Alcazar, Mourad Chioua, Jose Marco-Contelles, Ricardo Martinez-Murillo, Milagros Ramos, Gustavo V. Guinea, Daniel Gonzalez-Nieto
Summary: Brain stroke is a common pathology that causes disability in older adults. Previous neuroprotective treatments have not been effectively translated into clinical practice, highlighting the need for novel targets. This study demonstrates the neuroprotective properties of 2-APB and its ability to improve cerebral blood perfusion by targeting spreading depolarization events in ischemic stroke.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Kobkaew Bumroongthai, Dean P. J. Kavanagh, Paul Genever, Neena Kalia
Summary: This study demonstrates that 3D culture of specific bone marrow-derived MSC sub-populations improves their homing and protective effects on coronary blood vessels in the injured heart. Compared to conventional 2D culture, 3D culture enhances the adhesive behavior of MSCs and reduces neutrophil events in the coronary capillaries. Additionally, the combination of dual therapy with heparin further enhances the vasculoprotective efficacy. The findings highlight the importance of 3D culture and specific MSC sub-populations in improving therapeutic outcomes for reperfusion injury.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
M. Marini, A. Nardini, R. Martinez Vazquez, C. Conci, M. Bouzin, M. Collini, R. Osellame, G. Cerullo, B. S. Kariman, M. Farsari, E. Kabouraki, M. T. Raimondi, G. Chirico
Summary: Non-linear excitation microscopy has advantages over conventional confocal techniques for in-vivo imaging, but tissue penetration can still be problematic. This study develops and tests fibroblast cell culture plano-convex microlenses for non-linear imaging of biological tissue. The microlenses can be used individually or in an array, and they provide magnified fluorescence images without substantially affecting the signal-to-noise ratio. These results pave the way for optical in-vivo inspection of biological processes using implanted micro-optics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Multidisciplinary Sciences
Takahiro Tsuji, Hiroaki Ozasa, Wataru Aoki, Shunsuke Aburaya, Tomoko Yamamoto Funazo, Koh Furugaki, Yasushi Yoshimura, Masatoshi Yamazoe, Hitomi Ajimizu, Yuto Yasuda, Takashi Nomizo, Hironori Yoshida, Yuichi Sakamori, Hiroaki Wake, Mitsuyoshi Ueda, Young Hak Kim, Toyohiro Hirai
NATURE COMMUNICATIONS
(2020)
Review
Neurosciences
Daisuke Kato, Hiroaki Wake
Summary: Oligodendrocytes, which form myelin sheaths, play a key role in regulating conduction velocity and white matter structure changes during learning and training. Recent studies highlight the importance of activity-dependent changes in white matter for learning and behavior, shedding light on potential therapeutic targets for diseases with myelin impairment.
NEUROSCIENCE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Kana Ozaki, Daisuke Kato, Ako Ikegami, Akari Hashimoto, Shouta Sugio, Zhongtian Guo, Midori Shibushita, Tsuyako Tatematsu, Koichiro Haruwaka, Andrew J. Moorhouse, Hideto Yamada, Hiroaki Wake
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Takuya Okada, Daisuke Kato, Yuki Nomura, Norihiko Obata, Xiangyu Quan, Akihito Morinaga, Hajime Yano, Zhongtian Guo, Yuki Aoyama, Yoshihisa Tachibana, Andrew J. Moorhouse, Osamu Matoba, Tetsuya Takiguchi, Satoshi Mizobuchi, Hiroaki Wake
Summary: This research utilizes multiphoton in vivo imaging and holographic stimulation to explore the changes in neuronal activity and connectivity within S1 during sustained pain, revealing the formation of pain circuits. Increasing neuronal activity and synchrony artificially can reduce pain thresholds, while locally blocking N-type Ca2+ channels can decrease synchrony and allodynia associated with inflammatory pain. Targeting these S1 pain circuits may offer new ways to alleviate inflammatory pain.
Review
Neurosciences
Xiangyu Quan, Daisuke Kato, Vincent Daria, Osamu Matoba, Hiroaki Wake
Summary: Combining holographic structured illumination with optogenetics enables patterned stimulation of neurons and glial cells in a living brain, and in vivo functional imaging allows for visualization of cellular responses. Integration of these techniques can verify the link between cell function and behavior output, but there are technical limitations to optogenetic stimulation of multiple cells.
NEUROSCIENCE RESEARCH
(2022)
Editorial Material
Neurosciences
Masanori Murayama, Hiroaki Wake
NEUROSCIENCE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Anri Hirai, Shouta Sugio, Collins Nimako, Shouta M. M. Nakayama, Keisuke Kato, Keisuke Takahashi, Koji Arizono, Tetsushi Hirano, Nobuhiko Hoshi, Kazutoshi Fujioka, Kumiko Taira, Mayumi Ishizuka, Hiroaki Wake, Yoshinori Ikenaka
Summary: Neonicotinoid pesticides have harmful effects on bees and dragonflies, possibly related to neurodevelopmental disorders. In vivo Ca2+ imaging can detect abnormal activity of neuronal circuits in the brain caused by neonicotinoid application, providing further understanding of their potential harm.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Daisuke Kato, Xiangyu Quan, Yuta Tanisumi, Zhongtian Guo, Mitsuhiro Morita, Tetsuya Takiguchi, Osamu Matoba, Hiroaki Wake
Summary: Recent advances in optical bioimaging and optogenetics have enabled the visualization and manipulation of neural activity in living animals. This study presents a high spatiotemporal resolution microscope that combines optogenetics with digital holographic technology to visualize and manipulate neural activity. These protocols provide accurate spatiotemporal information on neural activity, which may be useful for understanding the pathogenesis of neuropsychiatric disorders.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Review
Neurosciences
Shouta Sugio, Daisuke Kato, Hiroaki Wake
Summary: Each oligodendrocyte forms myelin in multiple axons to regulate conduction velocity and coordinate information transfer in the central nervous system. Recent studies have challenged the traditional view of static myelin by demonstrating its plasticity throughout life, especially during the acquisition of new motor skills and the formation of memory. These plastic changes in myelin optimize the temporal pattern of neural circuit activity associated with skill learning and memory.
NEUROSCIENCE RESEARCH
(2023)
Article
Multidisciplinary Sciences
Norihiro Imai, Yuki Ohsaki, Jinglei Cheng, Jingjing Zhang, Fumitaka Mizuno, Taku Tanaka, Shinya Yokoyama, Kenta Yamamoto, Takanori Ito, Yoji Ishizu, Takashi Honda, Masatoshi Ishigami, Hiroaki Wake, Hiroki Kawashima
Summary: Lipid droplets (LDs) have been observed in the nuclei of hepatocytes, but their significance in liver disease remains unresolved. This study aimed to explore the pathophysiological features of intranuclear LDs in liver diseases.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Yuki Hattori, Daisuke Kato, Futoshi Murayama, Sota Koike, Hisa Asai, Ayato Yamasaki, Yu Naito, Ayano Kawaguchi, Hiroyuki Konishi, Marco Prinz, Takahiro Masuda, Hiroaki Wake, Takaki Miyata
Summary: By tracking cells, researchers found that CD206+ macrophages frequently enter the inner surface of the mouse cerebral wall and convert into microglia at embryonic day 12. It was also discovered that intraventricular macrophages are supplied transepithelially from the roof plate. This study demonstrates that the roof plate->ventricle->pallium route is essential for microglial colonization into the embryonic mouse brain.
Article
Cell Biology
Akari Hashimoto, Nanami Kawamura, Etsuko Tarusawa, Ikuko Takeda, Yuki Aoyama, Nobuhiko Ohno, Mio Inoue, Mai Kagamiuchi, Daisuke Kato, Mami Matsumoto, Yoshihiro Hasegawa, Junichi Nabekura, Anne Schaefer, Andrew J. Moorhouse, Takeshi Yagi, Hiroaki Wake
Summary: Cross-modal plasticity refers to the repurposing of brain regions to improve other sensory modalities. In this study, it was found that visual deprivation induced cross-modal plasticity by rewiring the microglia-mediated local circuit synapse. This resulted in enhanced excitatory responses in the secondary lateral visual cortex (V2L) during whisker stimulation and improved vibrissae sensory discrimination. The enhanced cross-modal response was achieved by the selective removal of inhibitory synapse terminals on pyramidal neurons by microglia via the matrix metalloproteinase 9 signaling pathway. These findings provide insights into how cortical circuits integrate different inputs to compensate for neuronal damage.
Review
Neurosciences
Rosa C. Paolicelli, Amanda Sierra, Beth Stevens, Marie-Eve Tremblay, Adriano Aguzzi, Bahareh Ajami, Ido Amit, Etienne Audinat, Ingo Bechmann, Mariko Bennett, Frederick Bennett, Alain Bessis, Knut Biber, Staci Bilbo, Mathew Blurton-Jones, Erik Boddeke, Dora Brites, Bert Brone, Guy C. Brown, Oleg Butovsky, Monica J. Carson, Bernardo Castellano, Marco Colonna, Sally A. Cowley, Colm Cunningham, Dimitrios Davalos, Philip L. De Jager, Bart de Strooper, Adam Denes, Bart J. L. Eggen, Ukpong Eyo, Elena Galea, Sonia Garel, Florent Ginhoux, Christopher K. Glass, Ozgun Gokce, Diego Gomez-Nicola, Berta Gonzalez, Siamon Gordon, Manuel B. Graeber, Andrew D. Greenhalgh, Pierre Gressens, Melanie Greter, David H. Gutmann, Christian Haass, Michael T. Heneka, Frank L. Heppner, Soyon Hong, David A. Hume, Steffen Jung, Helmut Kettenmann, Jonathan Kipnis, Ryuta Koyama, Greg Lemke, Marina Lynch, Ania Majewska, Marzia Malcangio, Tarja Malm, Renzo Mancuso, Takahiro Masuda, Michela Matteoli, Barry W. McColl, Veronique E. Miron, Anna Victoria Molofsky, Michelle Monje, Eva Mracsko, Agnes Nadjar, Jonas J. Neher, Urte Neniskyte, Harald Neumann, Mami Noda, Bo Peng, Francesca Peri, V. Hugh Perry, Phillip G. Popovich, Clare Pridans, Josef Priller, Marco Prinz, Davide Ragozzino, Richard M. Ransohoff, Michael W. Salter, Anne Schaefer, Dorothy P. Schafer, Michal Schwartz, Mikael Simons, Cody J. Smith, Wolfgang J. Streit, Tuan Leng Tay, Li-Huei Tsai, Alexei Verkhratsky, Rommy von Bernhardi, Hiroaki Wake, Valerie Wittamer, Susanne A. Wolf, Long-Jun Wu, Tony Wyss-Coray
Summary: Microglial research has made significant progress, but the current classification system fails to accurately describe their diversity, leading to misconceptions about their functions. To address this issue, a group of multidisciplinary experts has proposed a naming framework and recommendations to help researchers better understand and describe the different states and functions of microglia.
Article
Veterinary Sciences
Misaki Nishi, Shouta Sugio, Tetsushi Hirano, Daisuke Kato, Hiroaki Wake, Asuka Shoda, Midori Murata, Yoshinori Ikenaka, Yoshiaki Tabuchi, Youhei Mantani, Toshifumi Yokoyama, Nobuhiko Hoshi
Summary: This study found that exposure to the neonicotinoid pesticide clothianidin (CLO) leads to overactivation of neurons and disruption of neuronal homeostasis, potentially inducing neuroinflammation and affecting synaptic activity.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2022)
Article
Medicine, Research & Experimental
Yu Hirata, Kazuhiro Nomura, Daisuke Kato, Hiroaki Wake, Wataru Ogawa, Yu Hirata, Yoshihisa Tachibana, Takahiro Niikura, Kana Uchiyama, Tetsuya Hosooka, Tomoaki Fukui, Keisuke Oe, Ryosuke Kuroda, Yuji Hara, Takahiro Adachi, Koji Shibasaki
Summary: This study reveals that the upregulation of KLF15 and IL-6, as well as the decrease in intracellular Ca2+ concentration, are involved in muscle atrophy induced by immobility. This finding has been validated in both mouse models and human samples.
JOURNAL OF CLINICAL INVESTIGATION
(2022)