Journal
BRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY
Volume 52, Issue 2, Pages 134-139Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.bjoms.2013.10.004
Keywords
Peripheral blood CD34+cells; Mesenchymal stem cells; Co-culture; Bone regeneration; Rabbit calvarial critical-size defect; Cell sheet transplantation
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Funding
- National Natural Science Foundation of China [31070873, 81170938]
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The reconstruction of large bony defects remains a clinical challenge, and angiogenesis and neovascularisation are being given more attention in bone tissue engineering. In this study we cocultured peripheral blood CD34+ cells (PB-CD34+ cells), an endothelial progenitor cell/haematopoietic stem cell-enriched population, with bone marrow-derived mesenchymal stem cells (MSC) to investigate their potential for bony regeneration. Cocultured cells showed better osteogenic differentiation than MSC alone in vitro. The cocultured cells and MSC sheets were also composited with hydroxyapatite and implanted in calvarial critical-size defects in rabbits. The rabbits were killed before microcomputed tomographic (MicroCT) and histological analysis. The results showed that cocultured cell composites had promoted bony regeneration more efficiently by 8 weeks after implantation. Our results indicate that the coculture of PB-CD34+ cells and MSC increases bony regeneration in calvarial critical-size defects in rabbits, and provide a new promising therapeutic strategy to aid skeletal healing. (C) 2013 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
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