Journal
BRITISH JOURNAL OF NUTRITION
Volume 108, Issue 12, Pages 2148-2157Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114512000396
Keywords
Salmo salar L.; Myogenesis; Refeeding and fasting; Nutrient availability; Insulin-like growth factor system
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Funding
- Foundation for Science and Technology (FCT)
- Biotechnology and Biological Research Council [BB/D015391/1]
- BBSRC [BB/D015391/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D015391/1] Funding Source: researchfish
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We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon (Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12 degrees C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: -12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1-6 h after feeding and emptied within 48-96 h. IGF-I, MyoD1c, MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1.
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