Journal
BRITISH JOURNAL OF HAEMATOLOGY
Volume 148, Issue 3, Pages 441-444Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2141.2009.07986.x
Keywords
multipotent adult progenitor cells; B104; cell culture; cell growth; proliferation
Categories
Funding
- Patrick Berthoud Clinical Research
- Burden Trust
- Ministerio de Sanidad [PI050168]
- ISCIII-RETIC [RD06/0014]
- UTE
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P>This report describes the isolation of rodent multipotent adult progenitor cells (MAPCs) and proliferation of these cells in both standard medium and medium without exogenous serum or growth factors conditioned by the rat cell line B104. MAPCs have exacting requirements for their proliferation in vitro but once established proliferate rapidly at low seeding density, requiring almost daily passage and media exchange. Previously published methods for growth of MAPCs in vitro all used media supplemented with serum and growth factors, which adds considerable expense.
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