Journal
BRITISH JOURNAL OF HAEMATOLOGY
Volume 144, Issue 1, Pages 107-115Publisher
WILEY
DOI: 10.1111/j.1365-2141.2008.07429.x
Keywords
minimal residual disease; childhood Acute Lymphoblastic Leukaemia; real time quantitative PCR; flow cytometry; MRC UKALL 97/99
Categories
Funding
- Children's Leukaemia Research Project
- Children's Cancer Fund
- Our Lady's Children's Hospital, Dublin, Ireland
- Bone Marrow for Leukaemia Trust
- St. James's Hospital, Dublin, Ireland
- Irish Clinical Oncology Research Group (ICORG)
- Translational Disease Specific Subgroup of ICORG
Ask authors/readers for more resources
In this single centre study of childhood acute lymphoblastic leukaemia (ALL) patients treated on the Medical Research Council UKALL 97/99 protocols, it was determined that minimal residual disease (MRD) detected by real time quantitative polymerase chain reaction (RQ-PCR) and 3-colour flow cytometry (FC) displayed high levels of qualitative concordance when evaluated at multiple time-points during treatment (93.38%), and a combined use of both approaches allowed a multi time-point evaluation of MRD kinetics for 90% (53/59) of the initial cohort. At diagnosis, MRD markers with sensitivity of at least 0.01% were identified by RQ-PCR detection of fusion gene transcripts, IGH/TRG rearrangements, and FC. Using a combined RQ-PCR and FC approach, the evaluation of 367 follow-up BM samples revealed that the detection of MRD >1% at Day 15 (P = 0.04), >0.01% at the end of induction (P = 0.02), >0.01% at the end of consolidation (P = 0.01), >0.01% prior to the first delayed intensification (P = 0.01), and >0.1% prior to the second delayed intensification and continued maintenance (P = 0.001) were all associated with relapse and, based on early time-points (end of induction and consolidation) a significant log-rank trend (P = 0.0091) was noted between survival curves for patients stratified into high, intermediate and low-risk MRD groups.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available