Article
Biology
Oi Wah Liew, Samantha S. M. Ling, Shera Lilyanna, Yue Zhou, Peipei Wang, Jenny P. C. Chong, Yan Xia Ng, Angeline E. S. Lim, Eliot R. Y. Leong, Qifeng Lin, Teck Kwang Lim, Qinsong Lin, Enoch M. W. Ng, Tuck Wah Ng, A. Mark Richards
Summary: Liew et al. present an efficient monoclonal antibody (mAb) production method, successfully developing mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for antibody panel generation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Siming Zhu, Yi Tang, Youxiang Diao
Summary: This study prepares monoclonal antibodies against Tembusu virus (TMUV) and identifies their epitopes. The antibodies show specific binding and can be used for various detection methods. This research contributes to the understanding of TMUV and lays the foundation for further studies.
Article
Microbiology
Matthew Slarve, Zeferino Reyna, Elizabeth Burk, Juan Ruiz-Delgado, Rachel Li, Jun Yan, Brian Luna, Brad Spellberg
Summary: Researchers have identified the antibody MAb5, which demonstrates broad binding against both U.S. and international isolates of A. baumannii and shows protective efficacy in vivo.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Andreza Alves Belo, Dayane L. Naves de Souza, Marcella Nunes De Melo-Braga, Leticia Lopes de Souza, Denis A. Molina Molina, Patricia D. Vaz de Melo, Martin R. Larsen, Clara Guerra-Duarte, Carlos Chavez-Olortegui
Summary: This study successfully generated a neutralizing antibody against Bothrops snake venom in Brazil, specifically targeting its coagulant activity. The use of monoclonal antibodies for immunoaffinity chromatography proved to be an effective technique for purifying bioactive toxins from the venom.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Elaine Thai, Rajagopal Murugan, Spela Binter, Clare Burn Aschner, Katherine Prieto, Audrey Kassardjian, Anna S. Obraztsova, Ryu Won Kang, Yevel Flores-Garcia, Shamika Mathis-Torres, Kan Li, Gillian Q. Horn, Richard H. C. Huntwork, Judith M. Bolscher, Marloes H. C. de Bruijni, Robert Sauerwein, S. Moses Dennison, Georgia D. Tomaras, Fidel Zavala, Paul Kellam, Hedda Wardemann, Jean-Philippe Julien
Summary: IGHV3-33-encoded antibodies play a crucial role in the human immune response against Plasmodium falciparum. This study investigated the molecular basis of antibody cross-reactivity and its relationship with efficacy. The findings provide insights into the development of antibody-based interventions against malaria.
Article
Hematology
Xia Wang, Biao Liu, Mengqiao Xu, Yizhi Jiang, Jundong Zhou, Jun Yang, Haidi Gu, Changgeng Ruan, Jinchang Wu, Yiming Zhao
Summary: The study revealed that high expression of PDPN in tumors is associated with an increased risk of VTE, inducing thrombosis through platelet activation. The anti-PDPN antibody SZ-168 can inhibit PDPN-induced platelet activation.
THROMBOSIS RESEARCH
(2021)
Article
Microbiology
Anna Malyshkina, Wibke Bayer, Philip Podschwadt, Lucas Otto, Zehra Karakoese, Kathrin Sutter, Kirsten Bruderek, Baoxiao Wang, Kerry J. Lavender, Mario L. Santiago, Pia Madeleine Leipe, Carina Elsner, Stefan Esser, Sven Brandau, Matthias Gunzer, Ulf Dittmer
Summary: Antiretroviral therapy transformed HIV into a chronic disease, but eliminating the virus remains challenging. Inducing cytotoxic Tfh through immunotherapy may be a novel approach to reduce viral load and delay virus rebound.
Article
Biochemistry & Molecular Biology
Guangwei Han, Haotian Yang, Huiling Xu, Suya Zheng, Yingxiang Li, Zehui Liu, Fang He
Summary: Novel antiviral peptides derived from pCD163 were developed, which showed inhibitory effects on PRRSV of different lineages and modulated the NF-kappa B pathway. These findings deepen the understanding of the interaction between PRRSV and pCD163 receptor, and provide alternative universal antiviral strategies against PRRSV.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Anouk Smet, Joao Paulo Portela Catani, Tine Ysenbaert, Amanda Goncalves, Harry Kleanthous, Thorsten U. Vogel, Xavier Saelens, Emma R. Job
Summary: This study demonstrates that NA-specific antibodies can interfere with the replication of A(H1N1)pdm09 in human airway epithelial cells, blocking viral entry and egress. Furthermore, human sera with neuraminidase inhibition activity against A(H1N1)pdm09 N1 can also reduce H6N1 virus infection in HAE cells.
Article
Veterinary Sciences
Kexin Zhu, Dong Yu, Jiahui An, Yufeng Li
Summary: In this study, a monoclonal antibody (mAb) was discovered that can be used to prevent and control G. parasuis infection in vivo and in vitro, suggesting that Fe (3+) ABC transporter substrate-binding protein is an immunodominant antigen and a promising candidate for subunit vaccine development.
VETERINARY RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Fan Yang, Sijing Yan, Linwei Zhu, Frederick X. C. Wang, Fumin Liu, Linfang Cheng, Hangping Yao, Nanping Wu, Rufeng Lu, Haibo Wu
Summary: This study generated a panel of neutralizing antibodies against A(H1N1) pdm09 influenza virus. Eleven antibodies demonstrated therapeutic protection in a mouse model of A(H1N1) pdm09 infection, and a bispecific antibody combining the specificities of two antibodies also showed effectiveness in preventing and treating A(H1N1) pdm09 infection.
ANTIVIRAL RESEARCH
(2022)
Article
Medicine, Research & Experimental
Kuan-Ying A. Huang, Daming Zhou, Tiong Kit Tan, Charles Chen, Helen M. E. Duyvesteyn, Yuguang Zhao, Helen M. Ginn, Ling Qin, Pramila Rijal, Lisa Schimanski, Robert Donat, Adam Harding, Javier Gilbert-Jaramillo, William James, Julia A. Tree, Karen Buttigieg, Miles Carroll, Sue Charlton, Chia-En Lien, Meei-Yun Lin, Cheng-Pin Chen, Shu-Hsing Cheng, Xiaorui Chen, Tzou-Yien Lin, Elizabeth E. Fry, Jingshan Ren, Che Ma, Alain R. Townsend, David Stuart
Summary: The study reveals that representative antibodies targeting non-overlapping epitopes are effective against wild type virus and emerging variants. The neutralization is associated with the inhibition of viral RBD binding to ACE2. Structural analysis shows that these antibodies have unique features while sharing some similarities with previously reported neutralizing monoclonal antibodies.
Article
Plant Sciences
Kaewta Rattanapisit, Tharita Kitisripanya, Atthaphon Konyanee, Worapol Sae-Foo, Apisit Burapapiruin, Waraporn Putalun, Seiichi Sakamoto, Waranyoo Phoolcharoen, Gorawit Yusakul
Summary: The plant expression platform is a new source for producing IgG targeting small low-molecular-weight molecules. The study demonstrated that plant-produced functional IgG maintained the binding activity and specificity of IgG derived from mammalian cells, showing potential as an efficient and cost-effective platform for antibody production in immunoassays targeting low-molecular-weight compounds.
PLANT CELL REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Pardis Mokhtary, Zeinab Pourhashem, Akram Abouei Mehrizi, Claudia Sala, Rino Rappuoli
Summary: Monoclonal antibodies are a revolutionary class of medications that can bind specific targets and receptors to combat various diseases. New technologies, such as gene delivery, have been developed to improve their efficacy and delivery routes, enabling them to be used in the treatment of viral infections and outbreaks.
Article
Immunology
Philippe J-L Y. Gevenois, Pieter De Pauw, Steve Schoonooghe, Cedric Delporte, Thami Sebti, Karim Amighi, Serge Muyldermans, Nathalie Wauthoz
Summary: IL-13, a pleiotropic cytokine mainly secreted by Th2 cells, has been extensively studied for its role in conditions involving allergy, inflammation, and fibrosis. While traditional anti-IL-13 mAbs have not shown significant improvement in human trials, nanobodies derived from camelid H chain-only Abs have shown promise in targeted therapy with improved affinity and biological activity.
JOURNAL OF IMMUNOLOGY
(2021)
