Journal
BRITISH JOURNAL OF HAEMATOLOGY
Volume 142, Issue 5, Pages 831-835Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2141.2008.07256.x
Keywords
haematopoietic stem cells; cell movement; stromal cell-derived factor-1 alpha; protein kinase C-a alpha; cultivation
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Addition of the inflammatory cytokine interleukin (IL)-6 to the culture medium of human cord blood haematopoietic stem and progenitor cells (HSPCs) has been shown to lead to an altered stromal cell-derived factor-1 alpha-dependent migratory phenotype. This study investigated whether this effect was attributed to a differential engagement of protein kinase C (PKC) isotypes. The migratory activity of both Flt3-ligand and Flt3-ligand/IL-6 cultured cord blood HSPCs was PKC-alpha dependent on day 1, but PKC-alpha independent after 5 d of cultivation. PKC-alpha expression was not down-regulated in cells cultured for 5 d indicating a switch of signalling molecules directing cell migration.
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